Rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof

A protein and disease resistance technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as loss of variety resistance
CN109369790AActive Publication Date: 2019-02-22INST OF CROP SCI CHINESE ACAD OF AGRI SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2019-02-22

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Abstract

The invention discloses rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof. The rice bacterial blight resistance related proteins OsBBR1 are following (A1), (A2) or (A3), wherein (A1) the proteins have the amino acid sequence of the 240-438 bits in the third sequence, (A2) the proteins are obtained in the process that the amino acid sequence shownin the 240-438 bits of the third sequence in a sequence table is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, and have the same function, and (A3)the fusion proteins are obtained by connecting labels to the N ends or / and the C ends of the (A1) or the (A2). Experiments show that plants transfecting OsBBR1 genes can significantly improve diseaseresistance of plants, the disease resistance of the plants missing the OsBBR1 protein function after gene editing is lowered, which indicates that the OsBBR1 proteins and the coding genes thereof canbe used for improving the disease resistance of the plants and have great significance for cultivating the disease-resistant plants.
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Description

technical field

[0001] The invention relates to rice bacterial blight resistance-related protein OsBBR1 and its coding gene and application in the field of biotechnology. Background technique

[0002] Rice bacterial blight is an important bacterial disease in rice cultivation all over the world, and it is seriously harmful in the rice regions of South China and Southeast Asia. In normal years, rice production can be reduced by about 10%, and in severe cases, it can be reduced by 50%-60%. Breeding disease-resistant varieties with resistance genes is currently the most economical and effective measure to control rice bacterial blight. So far, 42 rice bacterial blight resistance genes have been reported at home and abroad (http: / / www.shigen.nig.ac. jp / rice / oryzabase / gene / list). However, the resistance genes derived from wild rice are difficult to use; some resistance genes only have adult plant resistance; most resistance genes have a narrow resistance spectrum. Among the id...

Claims

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