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Rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof

A protein and disease resistance technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as loss of variety resistance

Active Publication Date: 2019-02-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rice bacterial blight has complex diversity and high variability. Production practice shows that after large-scale promotion and planting of disease-resistant varieties carrying a single main effect gene, potentially toxic races will rise to dominant races or new pathogen mutations will emerge. Toxic small species, which can easily lead to loss of variety resistance

Method used

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  • Rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof
  • Rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof
  • Rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1, OsBBR1 can regulate rice bacterial blight resistance

[0107] This example provides a protein named OsBBR1 derived from the rice variety Wuyujing 20, its sequence is the 240-438th position of sequence 3 in the sequence table, and the CDS sequence of the OsBBR1 gene in the rice variety Wuyujing 20 is Sequence 2 in the sequence listing, and the genome sequence is Sequence 1 in the sequence listing.

[0108] 1. Construction of recombinant vector

[0109] 1. OsBBR1 gene expression vector pMDC43-BBR1

[0110] The CDS sequence of OsBBR1 was constructed on the expression vector pMDC43 through the Gateway system to obtain the expression vector pMDC43-BBR1 containing the OsBBR1 gene. The operation steps are as follows:

[0111](1) Extract the total RNA of the rice variety Wuyujing 20, reverse transcribe to obtain cDNA, use the cDNA as a template, use the forward primer OsBBR1-CDS-F:5'-ATGCGAGCTTCTCTCTCCCCACA-3' and the reverse primer OsBBR1-CDS -R: 5'-ATCCAGAAGCCA...

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Abstract

The invention discloses rice bacterial blight resistance related proteins OsBBR1 and coding genes and application thereof. The rice bacterial blight resistance related proteins OsBBR1 are following (A1), (A2) or (A3), wherein (A1) the proteins have the amino acid sequence of the 240-438 bits in the third sequence, (A2) the proteins are obtained in the process that the amino acid sequence shownin the 240-438 bits of the third sequence in a sequence table is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, and have the same function, and (A3)the fusion proteins are obtained by connecting labels to the N ends or / and the C ends of the (A1) or the (A2). Experiments show that plants transfecting OsBBR1 genes can significantly improve diseaseresistance of plants, the disease resistance of the plants missing the OsBBR1 protein function after gene editing is lowered, which indicates that the OsBBR1 proteins and the coding genes thereof canbe used for improving the disease resistance of the plants and have great significance for cultivating the disease-resistant plants.

Description

technical field [0001] The invention relates to rice bacterial blight resistance-related protein OsBBR1 and its coding gene and application in the field of biotechnology. Background technique [0002] Rice bacterial blight is an important bacterial disease in rice cultivation all over the world, and it is seriously harmful in the rice regions of South China and Southeast Asia. In normal years, rice production can be reduced by about 10%, and in severe cases, it can be reduced by 50%-60%. Breeding disease-resistant varieties with resistance genes is currently the most economical and effective measure to control rice bacterial blight. So far, 42 rice bacterial blight resistance genes have been reported at home and abroad (http: / / www.shigen.nig.ac. jp / rice / oryzabase / gene / list). However, the resistance genes derived from wild rice are difficult to use; some resistance genes only have adult plant resistance; most resistance genes have a narrow resistance spectrum. Among the id...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8282
Inventor 周永力卢家玲张帆黎志康
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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