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Spirulina polysaccharide and application thereof

A technology of spirulina polysaccharide and spirulina, applied in the fields of glycoengineering and glycobiology, can solve problems such as side effects and drug resistance, and achieve the effects of no toxic side effects, high efficiency and low price

Active Publication Date: 2016-06-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Tumor is a major problem facing human health in modern society. Chemical drugs have made important progress in inhibiting tumors, but there are still many shortcomings, such as drug resistance, side effects, etc.

Method used

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  • Spirulina polysaccharide and application thereof
  • Spirulina polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The extraction of embodiment 1 spirulina polysaccharide

[0031] 1. Take 10g of high-sugar spirulina algae powder, use water as the extraction solution, stir and dissolve until the spirulina is fully swollen (solid-to-liquid ratio 1:30), and homogenize under high pressure at room temperature, with a pressure of 80MPa, for 3 consecutive times.

[0032] 2. The high-pressure homogeneous solution was extracted with 80°C hot water extraction method for water-soluble polysaccharides from Spirulina. The extraction time was 4 hours. After the extraction, it was centrifuged at 6000 rpm for 20 minutes at high speed to remove the sediment and collect the supernatant.

[0033] 3. Use a flocculant to remove protein. The flocculant is polysilicate ferric sulfate and ZTC1+1 (component A and component B, produced by Beijing Zhongke Shengyuan Biotechnology Co., Ltd.), which are prepared separately 24 hours before use. 1% solution is added successively during flocculation, the amount of ...

Embodiment 2

[0036] The extraction of embodiment 2 spirulina polysaccharides

[0037] 1. Take 10g of high-sugar spirulina algae powder, use water as the extractant, stir and dissolve until the spirulina is fully swollen (solid-to-liquid ratio 1:40), homogenize under high pressure at room temperature, with a pressure of 80MPa, for 3 consecutive times.

[0038] 2. The high-pressure homogeneous solution was extracted with 90°C hot water extraction method for water-soluble polysaccharides from Spirulina. The extraction time was 4 hours. After extraction, it was centrifuged at 6000 rpm for 20 minutes at high speed to remove sediment and collect the supernatant.

[0039] 3. Use a flocculant to remove protein, the flocculant is iron polysilicate, the amount of the flocculant added is 2% of the volume of the polysaccharide solution, and the flocculation time is 2h.

[0040] 4. After flocculation, use high-speed centrifugation to remove the sediment. After collecting the supernatant, rotate and eva...

Embodiment 3

[0042] Example 3 Spirulina polysaccharide-induced tobacco resistance to tobacco mosaic virus test

[0043] (1) Spray treatment: the spirulina polysaccharide prepared in Example 1 is diluted with water respectively to obtain a solution with a concentration of 0.1 to 0.5 mg / mL, and then the whole plant is sprayed on the six to eight-leaf stage Tobacco spp. Three replicates, each with three plants; at the same time, the same concentration of laminarin was used as a control, and the blank group was sprayed with water.

[0044] (2) Preparation of virus inoculum: The source of the virus is the common strain preserved in our laboratory, the leaves of common tobacco infected with tobacco mosaic virus were removed from the main vein, and mixed with PBS (pH8.0, 0.01mol / L) at 1g / L Mix 20mL, add 0.015g corundum per 20mL of PBS, grind the leaves thoroughly, and obtain the inoculum.

[0045] (3) Inoculation: after 72 hours of spraying treatment of tobacco plants withered spot by the method...

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Abstract

The invention relates to glucose-rich spirulina polysaccharide and an application thereof in a plant green pesticide and an anti-tumor drug. High-sugar spirulina obtained through nutritional regulation is used as a raw material and is subjected to hot water extraction, protein removal with a flocculant, concentration, precipitation and freeze drying to obtain the water-soluble high-sugar spirulina polysaccharide having the molecular weight of 10-200 kDa; the spirulina polysaccharide comprises the monosaccharides mainly comprising rhamnose, fucose, arabinose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, and is characterized in that the glucose content is more than 50% in the monosaccharide composition. The spirulina polysaccharide can obviously induce plant disease resistance, has obvious anti-tumor effect on tumor, and can be used as the biological pesticide and the anti-tumor drug.

Description

technical field [0001] The invention relates to the research on polysaccharides and their biological activities, and studies in detail the biological activities and applications of glucose-rich spirulina polysaccharides in high-sugar spirulina regulated by nutrition, and belongs to the fields of glycoengineering and glycobiology. Background technique [0002] With the rapid development of agriculture, plant diseases are becoming more and more serious, which greatly affects the yield of agricultural plants. In order to control plant diseases, chemical fungicides are mainly used and resistant varieties are cultivated, but chemical fungicides can not only cause environmental pollution, but also endanger human health, and new resistant varieties also have long breeding time, high cost, and easy resistance. The development is slow due to reasons such as degeneration, so safe, environmentally friendly and fast methods for controlling plant diseases are imminent. [0003] Existing...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P1/00A01P3/00A61K36/02A61P35/00A23L1/09
Inventor 刘启顺尹恒孙永欣陈玮王文霞
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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