Streptomyces griseoplanus, application thereof and microbial agent

A technology of Streptomyces griseus and microbial inoculum, which is applied in the field of microorganisms, can solve the problems of limiting the application of phosphate-solubilizing actinomycetes and few studies on phosphate-solubilizing actinomycetes, etc., and achieves the effect of good phosphorus-solubilizing properties.

Active Publication Date: 2017-05-17
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Actinomycetes have attracted much attention because of their multiple functions such as tolerance to barrenness, strong survival in extreme environments, production of various active substances, and disease resistance and growth promotion. , limiting the application of phosphate-solubilizing actinomycetes

Method used

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  • Streptomyces griseoplanus, application thereof and microbial agent
  • Streptomyces griseoplanus, application thereof and microbial agent
  • Streptomyces griseoplanus, application thereof and microbial agent

Examples

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Effect test

Embodiment 1

[0050] The screening of embodiment 1 phosphate-dissolving actinomycetes

[0051] Weigh 10g of fresh corn rhizosphere soil sample collected from Gongzhuling and add it to 100mL of sterile water, prepare the soil dilution by plate dilution method, spread it on the NBRIP medium, cultivate it at 28°C for 48h, and pick the colony with good growth state purification. The purified strains were stored at 4°C and -80°C for future use. The screening results of phosphate-soluble actinomycetes were as follows: figure 1 shown. Named as PSA1 strain.

Embodiment 2

[0052] Embodiment 2 Phosphorus-dissolving performance measurement

[0053] The maize rhizosphere actinomycetes after the screening and purification of Example 1 were inoculated in the liquid Gaoshi No. 1 medium and carried out expanded culture for 7 days, and the thalline was collected by centrifugation at 8000rpm for 5min, and centrifuged at 8000rpm for 5min after resuspending the thallus in deionized water. The operation was repeated 3 times, and the components of Gaoshi No. 1 medium on the surface of the bacteria were washed, and 0.5 g of the bacteria (wet weight) was weighed aseptically and inoculated into 100 mL of NBRIP liquid medium (NBRIP medium: glucose 10 g, calcium phosphate 5g, magnesium chloride 5g, magnesium sulfate 0.25g, ammonium sulfate 0.1g, potassium chloride 0.2g, distilled water 1000mL, pH 7.0 ~ 8.0), 28 ℃ shaker culture 20d, using molybdenum antimony anti-colorimetric method to determine the fermentation of different culture time The content of available ...

Embodiment 3

[0055] The identification of embodiment 3 corn rhizosphere phosphorus-dissolving actinomycetes

[0056] 1. Identification of culture characteristics and physiological and biochemical characteristics of phosphate-solubilizing actinomycetes in maize rhizosphere

[0057] Adopt inserting method to observe embodiment 1 maize rhizosphere phosphorus-solubilizing actinomycetes mycelia morphology, and with reference to the method such as Fan Lixia, adopt International Streptomyces Program culture medium (ISP), the phosphorus-solubilizing actinomycetes bacterial strain is inoculated into yeast respectively Cream malt extract agar medium (ISP2), oat flour agar medium (ISP3), inorganic salt starch agar medium (ISP4), glycerol asparagine agar medium (ISP5), peptone yeast extract iron salt agar medium (ISP6 ), tyrosine agar medium (ISP7), Chapei medium and PDA medium, cultivated at 28°C for 5-10 days, observed and recorded the culture characteristics of the strains on 7 different media. Th...

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Abstract

The invention relates to the technical field of microbiology, and in particular to streptomyces griseoplanus, application thereof and a microbial agent. The preservation number of the streptomyces griseoplanus is CGMCC (China General Microbiological Culture Collection Center) No.13249. A research discovers that the streptomyces griseoplanus has better phosphorus dissolving characteristic and stronger siderophore synthesis ability, and the activity of multiple kinds of pathogenic fungi causing plant diseases can be inhibited. The streptomyces griseoplanus disclosed by the invention has plant disease resistance and growth promoting potential, and high-quality materials are provided for researching and developing environment-friendly type phosphorus dissolving plant growth promoting actinomyces fungi.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Streptomyces griseosa and its application and microbial agent. Background technique [0002] The increasing depletion of phosphate rock resources has become a worldwide problem restricting the sustainable development of agriculture. Phosphorus is an essential element for plant growth. In production practice, in order to increase crop yield, a large amount of soluble phosphorus fertilizers are applied to the soil, but most of them are fixed by the soil and exist in the form of insoluble inorganic phosphorus, which cannot be absorbed and utilized by plants. Therefore, the activation of soil invalid phosphorus has become an important problem urgently to be solved in resource science and fertilizer science. [0003] Phosphate-solubilizing bacteria can dissolve insoluble phosphate and activate the phosphorus state. It can not only "mobilize" farmland soil phosphorus pool resource...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C05G3/00C05F11/08C12R1/465
CPCA01N63/00C05F11/08C05G3/00C12N1/20C12N1/205C12R2001/465
Inventor 王呈玉赵成爱崔俊涛刘淑霞卢振兰窦森谢修鸿王玉军李莉马秀兰焉莉
Owner JILIN AGRICULTURAL UNIV
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