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Resistance-related protein and encoding gene thereof, and application of resistance-related protein and encoding gene for regulating and controlling plant disease resistance

A technology for transgenic plants and disease resistance, applied in the application field of regulating plant disease resistance, and can solve problems such as not being effectively controlled

Active Publication Date: 2015-04-29
PHEROBIO TECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country has paid attention to the research and prevention of Verticillium wilt since 1972, but it has not been effectively controlled so far

Method used

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  • Resistance-related protein and encoding gene thereof, and application of resistance-related protein and encoding gene for regulating and controlling plant disease resistance
  • Resistance-related protein and encoding gene thereof, and application of resistance-related protein and encoding gene for regulating and controlling plant disease resistance
  • Resistance-related protein and encoding gene thereof, and application of resistance-related protein and encoding gene for regulating and controlling plant disease resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1, disease resistance-associated protein VdAL improves the disease resistance of cotton

[0096] 1. Construction of transgenic VdAL cotton

[0097] 1. Construction of recombinant vectors and recombinant bacteria

[0098] The DNA molecule represented by the 1st-792nd nucleotides of sequence 2 in the sequence listing is artificially synthesized, which is the disease resistance related protein VdAL gene. The sequence between the Sal I and Kpn I recognition sites of the vector pSPT01 is replaced with the DNA molecule shown in the 1-792 nucleotides of sequence 2 in the sequence table (i.e. the disease resistance-related protein VdAL gene), keeping the other parts of pSPT01 The sequence remained unchanged, and a recombinant vector was obtained, which was named pSPT01-VdAL. The recombinant vector pSPT01-VdAL expresses the disease resistance-related protein VdAL shown in Sequence 1 in the Sequence Listing.

[0099] Among them, Sequence 2 consists of 864 nucleotide...

Embodiment 2

[0135] Example 2, the disease resistance-related protein VdAL improves the disease resistance of Arabidopsis

[0136] 1. Construction of transgenic Arabidopsis thaliana

[0137] 1. Construction of recombinant vectors and recombinant bacteria

[0138] The DNA molecule represented by the 1st-792nd nucleotides of sequence 2 in the sequence listing is artificially synthesized, which is the disease resistance related protein VdAL gene. The sequence between the Pst I and Kpn I recognition sites of the vector pCAMBIA1300-Super is replaced with the DNA molecule of the 1-792th nucleotide shown in sequence 2 in the sequence listing (i.e. the disease resistance-related protein VdAL gene), pCAMBIA1300- The other sequences of Super remained unchanged, and a recombinant vector was obtained, which was named pCAMBIA1300-Super-VdAL. The recombinant vector pCAMBIA1300-Super-VdAL expresses the protein shown in Sequence 1 in the Sequence Listing.

[0139] Among them, Sequence 2 consists of 864...

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Abstract

The invention discloses a resistance-related protein and an encoding gene thereof, and applications of the resistance-related protein and the encoding gene for regulating and controlling of plant disease resistance. The protein disclosed by the invention is protein a) or b) or c) or d): a) protein of 1-264th amino acids of which an amino acid sequence is a sequence 1; b) resistance-related protein which is obtained by substituting and / or missing and / or adding one or more amino acid residues to the amino acid sequence shown in the 1-264th amino acids in the sequence 1 in a sequence table; c) protein of which the amino acid sequence is the sequence 1; and d) resistance-related protein which is obtained by substituting and / or missing and / or adding one or more amino acid residues to the amino acid sequence of the sequence 1 in the sequence table. An experiment proves that the resistance-related protein and the encoding gene disclosed by the invention are used for improving the disease resistance of plants.

Description

technical field [0001] The invention relates to disease resistance-related proteins and their coding genes in the field of biotechnology and their application in regulating plant disease resistance. Background technique [0002] Cotton is an important economic crop in my country, and cotton textiles play a pivotal role in my country's export trade. Verticillium wilt is the most important disease of cotton, and the annual incidence area exceeds 3-4 million hm 2 , accounts for more than 70% of the entire cotton field area, generally reduces production by 20%-30%, and severely diseased fields reduce production by 60%-70% or even no harvest. The annual loss caused by Verticillium wilt reaches 1 million tons, and the fiber quality of cotton is severely reduced after the onset of the disease . At present, the upland cotton varieties (Gossypium hirsutum) planted in large areas in my country are difficult to achieve a high level of resistance to Verticillium wilt. Although Gossypi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N15/82A01H5/00C12N15/11A01N63/50
CPCC07K14/415C12N15/8205C12N15/8282A01N63/50C07K14/37C12N5/10C12N15/63A01N37/46
Inventor 齐俊生巩志忠洪旭辉王广兴张定朋
Owner PHEROBIO TECHNOLOGY CO LTD
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