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Small molecule compound for enhancing plant stress resistance

A plant stress resistance and compound technology, applied in the fields of biotechnology and botany, can solve the problems of inability to enhance plant seedling and mature drought tolerance, low application value, and low physiological activity

Inactive Publication Date: 2014-12-03
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Park, S.Y.et al.Science324, 1068-71 (2009) mentioned that the small molecule compound pyrabactin has a function similar to ABA, but its physiological activity is higher than that of ABA It is an order of magnitude lower, and can only bind to a few PYL receptors. It mainly acts as an ABA analogue in the seed germination stage, and cannot enhance the drought tolerance of plants in the seedling and mature stages, and has low application value in agricultural production.

Method used

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  • Small molecule compound for enhancing plant stress resistance
  • Small molecule compound for enhancing plant stress resistance
  • Small molecule compound for enhancing plant stress resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0128] Embodiment 1, small molecule compound AM1

[0129] 1. Structure

[0130] The small molecular compound AM1 (ABA mimic1) contained in the present invention has a different chemical structure from ABA, and consists of a xylene connected to a sulfonyl group and a quinoline derivative connected to an amino group, and has no optical activity, such as figure 1 a.

[0131] 2. In vitro biochemical experiments, PP2C protein phosphatase activity experiments

[0132] In vitro biochemical experiments show that AM1, as a broad-spectrum and highly efficient PYL receptor agonist, can bind to multiple PYL receptors, promote the latter's binding and inhibit the PP2C protein phosphatase activity.

[0133] The PP2C protein phosphatase activity experiment using SnRK2.6 phosphorylated polypeptide as the substrate showed that in the presence of AM1, multiple PYL receptors can bind to PP2C protein phosphatase (HAB1), thereby inhibiting the phosphorylation of SnRK2.6 by HAB1. De...

Embodiment 2

[0141] Example 2, the structure of small molecule compound AM1, PYL receptor protein and HAB

[0142] The inventors have detected the crystal structure of the PYL2-AM1-HAB1 complex. From the schematic diagram of the three-dimensional structure of the complex, the oxygen atom on the quinoline derivative of AM1 can pass through the free water molecule and the tryptophan at the 385th position of HAB1 (W385 ) to form a hydrogen bond, thereby further bringing HAB1 and PYL2 ( image 3 a) From the local schematic diagram of the two-dimensional structure of the complex, AM1 exists in the pocket structure of PYL2, and is connected to multiple active sites of PYL2 by hydrogen bonds through the oxygen atom on the sulfonyl group ( image 3 b). Therefore, AM1 binds in the pocket structure of PYL2, and binds to the key amino acid active sites of PYL2 and HAB1 through a mechanism similar to ABA, thereby further promoting and stabilizing the binding of PYL2 and HAB1. This result expla...

Embodiment 3

[0143] Example 3, AM1 can cause gene expression similar to ABA

[0144] The present inventors analyzed the effect of adding AM1 on gene expression in plants. Gene expression analysis showed that the addition of AM1 caused gene expression similar to that of ABA ( Figure 4 ). After 50 μM AM1 treatment, the expression levels of 6 known genes related to environmental stress induced by abscisic acid in 10-day-old wild-type Arabidopsis (Col-0) seedling plants were significantly increased, which was close to that after 50 μM ABA treatment. The expression level of the corresponding gene in the PYL receptor triple mutant strain (pyr1; pyl1; pyl4) has a limited increase. This result indicates that the induction of environmental stress-related genes by AM1 is through the ABA signal mediated by the PYL receptor. through the ( Figure 4 a).

[0145] The results of RNA sequencing (RNA-seq) showed that compared with the DMSO control group, the expression levels of about 4500 gen...

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Abstract

The invention relates to a small molecular compound for enhancing plant stress resistance, and firstly reveals that small molecular compound AM1 has similar physiological activity with ABA, can significantly enhance plant tolerance to abiotic stress (commonly including cold, drought, salinity, osmotic pressure, heat and the like) widely existing in environment, and has higher application value.

Description

technical field [0001] The invention belongs to the fields of biotechnology and botany; more specifically, the invention relates to a small molecular compound for enhancing plant stress resistance. Background technique [0002] Abscisic acid (ABA) is a key factor to balance the metabolism of plant endogenous hormones and related growth active substances. It has the ability to promote plants to absorb water and fertilizer in balance and coordinate metabolism in the body. It can effectively regulate the root / shoot and vegetative growth of plants. It plays an important role in improving the quality and yield of crops. By applying ABA, the application amount of chemical pesticides can be reduced, and it has important physiological activity and application value in many aspects such as improving the quality of agricultural products. In addition, exogenous ABA can cause the rapid closure of leaf stomata and inhibit transpiration, which can be used to preserve flowers or prevent w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/42A01P21/00
Inventor 曹民杰刘雪
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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