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Phytophthora sojae inhibitor and preparation method thereof

A technology of soybean Phytophthora and inhibitors, applied in the field of soybean Phytophthora inhibitors and its preparation, can solve the problems of killing beneficial microorganisms, plant phytotoxicity, human and animal poisoning, etc., and achieve improved resistance to Phytophthora soybean infection and safety High, low energy consumption effect

Active Publication Date: 2019-12-31
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Improper use of pesticides can cause phytotoxicity to plants, cause human and animal poisoning, kill beneficial microorganisms, lead to drug resistance of pathogens, and high residues of pesticides can also cause environmental pollution

Method used

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  • Phytophthora sojae inhibitor and preparation method thereof
  • Phytophthora sojae inhibitor and preparation method thereof
  • Phytophthora sojae inhibitor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A soybean Phytophthora inhibitor, characterized in that it includes propyl gallate and decyl alcohol, and decyl alcohol is used as a cosolvent for propyl gallate, and its dosage ratio is: 0.1mg-0.3mg of decyl alcohol: 1-2mmol Propyl gallate.

[0028] Such as Figure 1-7 As shown, the mechanism of Propyl gallate inhibiting Phytophthora sojae was excavated by proteomics: by adding gallic acid to the Phytophthora sojae treated with untreated Phytophthora sojae and propyl gallate (adding gallic acid in liquid V8 so that the final concentration was 0.5mmol / L) after 3-5 days of culture, the mycelium was collected, and liquid nitrogen was added to fully grind to powder. Each group of samples was added with 4 times the volume of powdered phenol extraction buffer (containing 1% protease inhibitor), and ultrasonically lysed. Add an equal volume of Tris-balanced phenol, centrifuge at 5500g for 10 min at 4°C, take the supernatant and add 5 times the volume of 0.1M ammonium acetat...

Embodiment 2

[0042] The propyl gallate is prepared by gallic acid and propanol under the catalysis of tannase, comprising the following preparation steps:

[0043] (1) Gallic acid extraction process: adopt supercritical carbon dioxide extraction method, weigh about 1.3-1.8 kg of blueberry leaves or green tea powder with a particle size of 2-5 mm, add it to 17-22% at a temperature of 2-8 Place in pure water at 5°C for 50-70 minutes, in supercritical carbon dioxide extraction, the extraction pressure of supercritical carbon dioxide is 23-27MPa, and the extraction time is 32-50 minutes, then gallic acid can be extracted;

[0044] (2) Tannase preparation process: Aspergillus niger was cultured in liquid potato medium for 3-5 days, the fermentation broth was collected by filtration, precipitated by ammonium sulfate precipitation, redissolved to extract crude protein, and ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 10kDa Filter out the ammonium sulfate and c...

Embodiment 3

[0056] Selection of solvents and preparation of inhibitory solutions: taking the inhibition rate of Phytophthora soybean as an index, from ethanol, propanol, butanol, pentanol, hexanol, acetone, dimethyl sulfoxide, ethyl acetate, petroleum ether and decanol, etc. Among the organic solvents, decanol is preferably used as a solubilizing agent for propyl gallate, and the dosage ratio is: 0.1mg-0.3mg of decyl alcohol: 1-2mmol of propyl gallate, after completely dissolving, add 60-70 500mL each of hot water at ℃ and water at room temperature can be used for the prevention and control of Phytophthora soybean. As shown in table 2

[0057]

[0058] Table 2. Solvent type and dosage optimization experiment table

[0059] Propyl gallate can also stimulate the defense response of plants and increase the expression of resistance genes to resist the infection of Phytophthora soybean. Reverse transcribe cDNA according to takara reagent and extract mRNA and proceed according to qRT-PCR s...

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Abstract

The invention provides a Phytophthora sojae inhibitor and a preparation method thereof, and relates to the field of plant disease biological prevention and control. According to the invention, the Phytophthora sojae inhibitor comprises propyl gallate, wherein propyl gallate as a food additive is mainly used for the oxidation resistance of food, the value EC50% of propyl gallate to Phytophthora sojae is only 0.458 mmol / L, ie., when the inhibition rate is 50%, the concentration of propyl gallate is only 0.458 mmol / L, so that the safety is high; the inhibition effects on Phytophthora sojae harm mainly comprise that propyl gallate can inhibit the growth, the action mechanism and the target site of Phytophthora sojae, wherein the proteomics technology reveals that the Phytophthora sojae inhibition mechanism is closely related to the great down-regulation of the protein expression in fat transport and metabolism, energy metabolism and carbohydrate metabolism and other key pathways; and the inhibition effects on Phytophthora sojae harm mainly comprise that propyl gallate can stimulate the defense reactions of plants so as to improve the expression quantity of resistance genes, such that the infection of Phytophthora sojae can be resisted.

Description

technical field [0001] The invention relates to the field of plant disease biological control, in particular to a soybean Phytophthora inhibitor and a preparation method thereof. Background technique [0002] Soybean blight caused by Phytophthora sojae is one of the devastating diseases in soybean production, and it is a class of phytosanitary dangerous pathogenic fungi announced by my country. The pathogen is mainly transmitted by soil. The pathogenic fungus of Phytophthora soybean is a typical soil resident fungus. It mainly overwinters in the soil and the host residue in the soil with highly resistant oospores and survives for a long time. When the temperature and humidity in the field are suitable, the oospores break dormancy and germinate to produce sporangia. When there is water accumulation after rain, the sporangia quickly release a large number of zoospores, and the zoospores are splashed by the rainwater to the surface of the host soil. Or by the host Attracti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/40C07C67/48C07C69/84C12P7/62A01P3/00
CPCA01N37/40C07C67/48C12P7/62C07C69/84Y02P20/54
Inventor 刘冬潘月敏周多奇吴彦吕新霞
Owner ANHUI AGRICULTURAL UNIVERSITY
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