Bacterial outer membrane vesicle carrier as well as preparation method and application thereof

A technology for outer membrane vesicles and bacteria, applied in the fields of biochemical equipment and methods, bacteria, chemical instruments and methods, etc., can solve the problem of reduced antigen uptake, and achieve the effect of promoting cross-presentation and inducing specific immune responses.
CN112773901AActive Publication Date: 2021-05-11THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Publication Date
2021-05-11

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Abstract

The invention provides a bacterial outer membrane vesicle carrier as well as a preparation method and application thereof. The invention provides a vaccine universal vector OMV-SpyC-SPAb which is constructed on the basis of a B structural domain SPAb of staphylococcus aureus protein A, is based on bacterial outer membrane vesicles and can target dendritic cells, the B structural domain SPAb of the staphylococcus aureus protein A is displayed on the bacterial outer membrane vesicles and can be rapidly combined with an antibody (such as an Anti-DEC205 antibody) of the targeted dendritic cells, and a novel OMV-OVA-DEC vaccine capable of targeting the dendritic cells and obviously enhancing antigen intake is prepared by using the SpyCatcher / SpyTag molecular glue to display the antigen on the surface of the bacterial outer membrane vesicles, so that the cross presentation of the dendritic cells on the antigen can be promoted, and the specific immune response can be induced, thereby achieving the anti-tumor purpose and having a wide application prospect.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a bacterial outer membrane vesicle carrier and its preparation method and application. Background technique

[0002] As a kind of tumor immunotherapy, tumor vaccine has a history of more than one hundred years, and has shown potential advantages and good application prospects in tumor immunotherapy. However, the immunogenicity of single antigen therapy is poor, which often leads to unsatisfactory anti-tumor immune effect. In order to solve this problem, the use of vaccine carriers can efficiently deliver antigens and adjuvants, and then enhance the antigen-specific adaptive immune response by improving the uptake, processing and presentation efficiency of antigens by dendritic cells (DC) . At present, liposomes, nanoparticles, nanomicelles, hydrogels, and biologically derived materials based on biological materials are used as vaccine carriers, which can improve the delivery effici...

Claims

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