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Polyelectrolyte hybrid hollow silica nanoparticles and its preparation method and application

A technology of silica and nanoparticles, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of difficult antigen cross-presentation, weak lysosome escape ability, antigen Unable to sustain release and other issues, to achieve the effect of enhancing antiviral or antitumor effect, increasing drug loading, and good monodispersity

Active Publication Date: 2020-09-08
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention solves the problem of poor monodispersity, low drug loading, unsustainable release of antigens, and weak lysosome escape ability when silica nanomaterials are applied to drug carriers and immune adjuvants in the prior art, making it difficult to realize the cross-presentation of antigens To solve the technical problems, a polyelectrolyte hybrid hollow silica nanoparticle and its preparation method and application are provided

Method used

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  • Polyelectrolyte hybrid hollow silica nanoparticles and its preparation method and application
  • Polyelectrolyte hybrid hollow silica nanoparticles and its preparation method and application
  • Polyelectrolyte hybrid hollow silica nanoparticles and its preparation method and application

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Embodiment 1

[0034] Preparation method of polyethyleneimine hybrid hollow mesoporous silica nanoparticles THMSNs, Figure 4 is a schematic diagram of the synthesis process, which specifically includes the following steps:

[0035] (1) Solid silica (sSiO 2 ) Preparation of nanoparticles: Synthesis of sSiO by sol-gel method 2 Mix nanoparticles, 50mL ethanol, 4mL deionized water and 2mL concentrated ammonia water, add 0.3mL TEOS, and stir for 6h in a water bath at 30°C to obtain sSiO 2 nanoparticles.

[0036] (2) Silica with a core-shell structure (sSiO 2 @mSiO 2 -micelle) nanoparticles: mix 110mL deionized water, 5mL ethanol and 600mg CTAB, then put the sSiO obtained in the previous step 2 Add the nanoparticles into the mixture, stir for 30 minutes, add 0.1mL TEOS, and react overnight in a water bath at 30°C to obtain sSiO 2 @mSiO 2 -micelle nanoparticles.

[0037] (3) Mesoporous silica (mSiO) with core-shell structure 2 @mSiO 2 ) Preparation of nanoparticles: the above solution is c...

Embodiment 2

[0040] The preparation method of polyethyleneimine hybrid hollow mesoporous silica nanoparticles THMSNs comprises the following steps:

[0041] (1) Solid silica (sSiO 2 ) Preparation of nanoparticles: Synthesis of sSiO by sol-gel method 2 Mix nanoparticles, 100mL ethanol, 8mL deionized water and 4mL concentrated ammonia water, add 3mL TEOS, and stir for 6h in a water bath at 30°C to obtain sSiO 2 nanoparticles.

[0042] (2) Silica with a core-shell structure (sSiO 2 @mSiO 2 -micelle) nanoparticles: mix 220mL deionized water, 10mL ethanol and 1200mg CTAB, then put the sSiO obtained in the previous step 2 Add the nanoparticles into the mixture, stir for 30 minutes, add 1.0mL TEOS, and react overnight in a water bath at 30°C to obtain sSiO 2 @mSiO 2 -micelle nanoparticles.

[0043] (3) Mesoporous silica (mSiO) with core-shell structure 2 @mSiO 2 ) Preparation of nanoparticles: centrifuge the above solution and disperse to 150mL concentration of 0.4M Na 2 CO 3 solution,...

Embodiment 3

[0046] The preparation method of polyethyleneimine hybrid hollow mesoporous silica nanoparticles THMSNs comprises the following steps:

[0047] (1) Solid silica (sSiO 2 ) Preparation of nanoparticles: Synthesis of sSiO by sol-gel method 2 Mix nanoparticles, 100mL ethanol, 8mL deionized water and 4mL concentrated ammonia water, add 5mL TEOS, and stir for 24h in a water bath at 30°C to obtain sSiO 2 nanoparticles.

[0048] (2) Silica with a core-shell structure (sSiO 2 @mSiO 2 -micelle) nanoparticles: mix 500mL deionized water, 20mL ethanol and 2500mg CTAB, then put the sSiO obtained in the previous step 2 Add the nanoparticles into the mixture, stir for 30 minutes, add 3.0mL TEOS, and react overnight in a water bath at 30°C to obtain sSiO 2 @mSiO 2 -micelle nanoparticles.

[0049] (3) Mesoporous silica (mSiO) with core-shell structure 2 @mSiO 2 ) Preparation of nanoparticles: centrifuge the above solution and disperse to 550mL concentration of 0.8M Na 2 CO 3 solution...

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Abstract

The invention provides polyelectrolyte hybrid hollow silica nano particles and a preparation method and application thereof. The insides of polyelectrolyte hybrid hollow silica nanoparticles are of hollow structures, and outer shell layers are hybrid structures of polyelectrolyte and silica; mesopores are formed in the shell layers. The preparation method comprises the steps of firstly synthesizing the silica nano particles with core-shell structures; then removing surfactant in the core-shell structures of the silica nano particles; finally etching the obtained mesoporous silica nano particles with the polyelectrolyte, thereby obtaining the polyelectrolyte hybrid hollow mesoporous silica nano particles. The preparation method is simple, and the obtained polyelectrolyte hybrid hollow mesoporous silica nano particles have good monodispersity, good biocompatibility and large specific surface area and pore volume, and can be applied to drug carriers and immunity adjuvant.

Description

technical field [0001] The invention belongs to the field of nano-biomedicine, and specifically relates to a polyelectrolyte hybrid hollow mesoporous silicon dioxide nanoparticle and a preparation method and application thereof. Background technique [0002] Vaccines have achieved great success in the prevention of infectious diseases and have shown great potential in the treatment of tumors. However, antigenic peptide vaccines generally have shortcomings such as small molecules, weak immunogenicity, and difficulty in inducing the body to produce an effective immune response, so certain substances are needed to enhance their immune effect. Immune adjuvants generally refer to a class of substances that can non-specifically enhance specific immunity by up-regulating immune cell surface molecules and promoting innate immune responses. Therefore, immune adjuvants have become an integral part of vaccines and play an important role in generating long-lasting protective immunity. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/34A61K47/04A61K39/39A61P35/00A61P31/12
CPCA61K9/5115A61K9/5146A61K39/39A61K2039/55555A61P31/12A61P35/00
Inventor 朱锦涛刘倩倩周雅捷陶娟张连斌李钰策殷小燕
Owner HUAZHONG UNIV OF SCI & TECH
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