Method for preparing environment-responsive mesoporous silicon nanoparticles

An environmentally responsive, nanoparticle technology, which is applied in non-active ingredient medical preparations, active ingredients-containing medical preparations, pharmaceutical formulas, etc., can solve problems such as strong cytotoxicity, and achieve good biocompatibility and good biocompatibility. Prospects of application, mild effect of reaction conditions

Inactive Publication Date: 2012-03-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, doxorubicin is a broad-spectrum anti-tumor drug, which can produce a wide range of biochemical effects on the body, but has strong cytotoxicity.

Method used

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  • Method for preparing environment-responsive mesoporous silicon nanoparticles
  • Method for preparing environment-responsive mesoporous silicon nanoparticles
  • Method for preparing environment-responsive mesoporous silicon nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] pH-responsive nanoparticle drug delivery system with regulated release of sphingosine phosphate

[0032] (1) Template extraction method to prepare mesoporous silicon nanoparticles, the template agent hexadecyltrimethylammonium bromide was dissolved in sodium hydroxide solution at 50 °C, and ethyl orthosilicate was added dropwise, and vigorously stirred to produce The white precipitate was dried by suction filtration, refluxed with ethanol for 8 hours to remove the template, dried in vacuum, and stored for later use;

[0033] (2) Disperse the mesoporous silicon prepared in step 1 in toluene, add 3-aminopropyltriethoxysilane, the molar ratio of silicon dioxide, 3-aminopropyltriethoxysilane and toluene is 5: 1:500, reacted under nitrogen protection for 20 h, dried to obtain amino-modified mesoporous silicon nanoparticles, and preserved for future use;

[0034] (3) Make the amino-modified mesoporous silicon prepared in step 2 into a suspension (1.0-2.0wt.%), add sodium alg...

Embodiment 2

[0037] Preparation of pH-responsive nanoparticles loaded with doxorubicin

[0038] (1) Template extraction method to prepare mesoporous silicon nanoparticles, the template agent hexadecyltrimethylammonium bromide was dissolved in sodium hydroxide solution at 95 ° C, and tetraethyl orthosilicate was added dropwise, and vigorously stirred to produce The white precipitate was dried by suction filtration, refluxed with ethanol for 24 hours to remove the template, dried in vacuum, and stored for future use;

[0039](2) Make a suspension (1.0-2.0wt.%) of the mesoporous silicon nanoparticles prepared in step 1, add polyallylamine hydrochloride (1-2mg / mL), stir at room temperature for 0.2-2h, 4000r / min Centrifuge for 3min, wash, disperse to make a suspension, add sodium polystyrene sulfonate solution (1-2mg / mL), stir at room temperature for 0.2-2h, centrifuge at 4000r / min for 3min, wash, disperse to make a suspension, repeat the whole process 4 times, carry out self-installation laye...

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Abstract

The invention relates to a method for preparing environment-responsive mesoporous silicon nanoparticles. The mesoporous silicon nanoparticles with the particle size of 20 to 1,000nm, the pore size of 2 to 50nm, the specific surface area of 500 to 1,500m<2> / g and a controlled structure are prepared by a template extraction method and subjected to surface functionalization by a self-assembly technology. A medicine sustained-release system prepared by the method is high in biocompatibility and medicine loading rate, can be released intelligently and controllably and has good application prospects in fields of growth factor and gene release, enzyme immobilization, cancer treatment and the like in tissue engineering, the preparation method is simple, reaction conditions are mild, and experiment raw materials are low in cost.

Description

technical field [0001] The invention belongs to the field of preparation of mesoporous silicon nanoparticles, in particular to a preparation method of environment-responsive mesoporous silicon nanoparticles. Background technique [0002] During tissue repair, the controlled release of growth factors plays an important role in seeding cell recruitment, modulating cell function, and promoting tissue healing. Most growth factors have short half-lives and variability in the body, and excessive release may cause toxicity and carcinogenicity. Therefore, the establishment of an effective controlled-release carrier is a necessary condition for realizing its biological function. In tissue engineering, two different growth factor loading methods are usually used: (1) chemically immobilize the growth factor on the carrier, and the growth factor and the carrier are combined by covalent bonds, hydrogen bonds or electrostatic interactions; (2) ) The physical embedding method coats growt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/04A61K9/14A61K31/704A61K31/661A61P35/00
Inventor 何创龙冯炜仇可新曹利军
Owner DONGHUA UNIV
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