The invention provides a prostate-specific membrane antigen (PSMA) targeting compound with a long circulation half-life period. The structure of the prostate-specific membrane antigen (PSMA) targeting compound is represented by a formula (I), wherein R1 is a prostate specific membrane antigen targeting compound; -(X)n-(CH2)m-(Y)q-, wherein X and Y are independently selected from lysine, glutamic acid or a derivative structure containing lysine and glutamic acid, n is an integer from 0 to 12, m is an integer from 0 to 60, q is an integer from 0 to 12, and each CH2 can be replaced by -O-, -NH(CO)- or -(CO)-NH- alone; L2 is -(CH2)p-, wherein p is an integer from 0 to 30, each CH2 can be substituted individually with -O-, -NH(CO)- or -(CO)-NH-; and R2 is a nuclide chelating group. The invention further provides a radioactive marker based on the structure of the compound; the compound and the radioactive marker have remarkably prolonged blood circulation half-life period and enhanced tumor uptake enrichment and retention time, and are suitable for nuclide treatment and imaging of PSMA high-expression tumors.