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Fusion protein antibody, enhanced vaccine and preparation method and application thereof

A fusion protein and antibody technology, applied in the field of immunity, to achieve high expression levels, promote IFN-γ secretion, and reduce costs

Inactive Publication Date: 2019-05-03
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the delivery of vaccines targeting CD317 for the treatment of chronic viral infections or tumors.

Method used

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  • Fusion protein antibody, enhanced vaccine and preparation method and application thereof
  • Fusion protein antibody, enhanced vaccine and preparation method and application thereof
  • Fusion protein antibody, enhanced vaccine and preparation method and application thereof

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Experimental program
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Effect test

Embodiment

[0041] 1. Construction of eukaryotic expression vector for HBs-α317scFv recombinant protein, the plasmid structure is as attached figure 2 (left) shown.

[0042] (1) The HBs coding gene is derived from the SH1203-C2 strain genome sequence (JX661494), with a total of 678 bases from 1550 to 2227 (the nucleotide sequence is SEQ ID NO: 14, and the corresponding amino acid sequence is SEQ ID NO: 6 shown). Synthesized by Invitrogen, an auxiliary sequence is added to the 5' end (the nucleotide sequence is SEQ ID NO: 9, and the corresponding amino acid sequence is shown in SEQ ID NO: 1) to facilitate vector transformation, protein secretion and expression, and the sequence includes EcoR1 enzyme digestion Site, Kozak sequence, signal peptide (human tissue-type plasminogen activator, tPA, NM_000930, 258-323) and other elements.

[0043] (2) The α317 scFv sequence was derived from a hybridoma cell (clone number 1C12C6) secreting anti-human and mouse CD317 monoclonal antibodies constru...

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Abstract

The invention relates to the technical field of immunization, and especially relates to a fusion protein antibody, an enhanced vaccine and a preparation method and an application thereof. The fusion protein antibody provided by the present invention comprises a secretion peptide, a targeting antigen, a connecting peptide, and a CD317-specific antibody. The CD317 as an antigen to target the delivery targets has more advantages that: 1) the expression level is higher and can be further induced up-regulated by IFN to form a benign feedback loop; and 2) the antigen presentation is more efficient and can activate the stronger specific immune response. Due to the high efficiency of antigen presentation, the amount of vaccine used can be reduced and the cost can be reduced. As the therapeutic vaccine, the ability to activate an immune response is stronger than that of a soluble non-targeting antigen under development. The experiments of the present invention show that the antibody provided bythe invention can significantly promote the division of spleen cells, promote the secretion of IFN-gamma, and improve the killing ability of mouse spleen cells to target cells.

Description

technical field [0001] The invention relates to the technical field of immunization, in particular to a fusion protein antibody, an enhanced vaccine and a preparation method and application thereof. Background technique [0002] The immune systems of patients with chronic viral infection and tumor patients are in a state of immune tolerance, and their ability to respond to conventional vaccines is weak, so they cannot achieve the purpose of curing the disease. Although great progress has been made in the design and application of vaccines with the development of biomedicine, at present, there is no real therapeutic vaccine for both chronic viral infections and tumor patients. [0003] Patients with chronic viral infection or tumor patients are immune insufficiency, and the body immunity is in a state of tolerance. Conventional soluble vaccines cannot induce a sufficiently strong immune response. Targeted vaccines can significantly enhance the body's immune response [see 1....

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62C12N15/85C12N5/10A61K39/385A61K39/29A61P31/20
Inventor 万晓春陈有海章桂忠刘曌刘绿艳
Owner SHENZHEN INST OF ADVANCED TECH
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