Monoclonal antibody TBEF3 of tubercle bacillus-resistant ESAT-6 and application thereof

A monoclonal antibody and Mycobacterium tuberculosis technology, applied in anti-bacterial immunoglobulin, biochemical equipment and methods, instruments, etc., can solve the problems of difficult promotion, cumbersome ELISPOT detection steps, expensive reagents, etc.
CN101985473AInactive Publication Date: 2011-03-16ZHEJIANG UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2011-03-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a monoclonal antibody TBEF3 of tubercle bacillus-resistant secretory antigen target protein in early stage. The monoclonal antibody TBEF3 is prepared by the following steps: selecting a specific antigen peptide mouse; collecting sensitized mouse B lymphocyte and mouse myeloma cell to fuse; judging positive clones by using an enzyme linked immunosorbent assayelisa and an immunoblotting testing method; establishing a hybridoma cell line TBEF3 for secreting monoclonal antibody of tubercle bacillus-resistant secretory ESAT-6 in early stage; carrying out multiplication culturing on the positive cells and injecting to congenic mouse enterocoelia for induction of generation of ascitic fluid containing antibody; and carrying out collection and antibody affinity purification to obtain the monoclonal antibody TBEF3 of tubercle bacillus-resistant secretory antigen target protein in early stage. The invention also provides an application of the monoclonal antibody in testing the secretory antigen target protein of mycobacterium tuberculosis in early stage.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to the preparation and application of a monoclonal antibody against mycobacterium tuberculosis early secretory antigen target protein (ESAT-6). Clone the hybridoma cell line of the antibody, induce ascites from the same strain of mice, prepare the anti-EAST-6 monoclonal antibody TBEF3, identify it as IgM, κ type, and then realize the antibody through affinity purification, electrophoresis, immunization and other technologies Applications. Background technique

[0002] Early diagnosis of tuberculosis is an important problem that has been plaguing the field of medical diagnosis. In the 20th century, due to the development and wide application of antibiotic research, humans successfully controlled tuberculosis. However, due to the continuous emergence and accumulation of drug resistance, Mycobacterium tuberculosis is still a real threat to human beings in the 21st century. More seriously,...

Claims

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