Stem cell microsphere gel compound for subcutaneous injection and application of stem cell microsphere gel compound

A subcutaneous injection and stem cell technology, which is applied in the field of stem cells and biomaterials, can solve problems such as the uncontrollable concentration of secreted cytokines and the instability of gel material degradation, and achieve good biocompatibility and stable degradability. The effect of stem cell activity

Inactive Publication Date: 2019-01-04
广东克瑞斯普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some shortcomings, which are manifested as the uncontrollable concentration of secreted cytokines and the instability of gel material degradation.

Method used

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Embodiment Construction

[0013] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0014] Preparation of stem cell-alginate microspheres:

[0015] Stem cell-alginate microspheres were prepared by electrostatic dripping method, with 2.5% (w / v) CaCl2 gel bath solution as the receiver. The surface of the needle and the gel bath was to inject 1.7% sodium alginate (w / v), 10 5 -10 6 Raw material solution of umbilical cord mesenchymal stem cells / mL (take P2-P5 frozen umbilical cord mesenchymal stem cells for resuscitation). Under the action of an electric field force with a voltage of 10.5kV, the raw material solution and the CaCl2 gel bath solution are cross-linked and solidified to form water-insoluble calcium alginate microspheres, and the particle diameter of the microspheres is 50-200 μm.

[0016] Hydrogel Preparation

[0017] Th...

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PUM

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Abstract

The invention relates to a stem cell microsphere gel compound for subcutaneous injection and a preparation method and application of the stem cell microsphere gel compound. The preparation method comprises the following specific steps: 1, separating umbilical cord mesenchymal stem cells; 2, preparing stem cell-alginate microspheres; 3, preparing hydrogel; 4, mixing the stem cell-alginate microspheres with the hydrogel, wherein the dispersion concentration of the stem cells in the hydrogen is 105-106 per mL; and 5, subcutaneously injecting the stem cell microsphere gel compound to a wound in atissue to repair tissue injury. A high polymer material such as hyaluronic acid and stem cells are embedded and combined through microspheres, the microspheres are dispersed into gel to form a biological scaffold material with a function of fixing cell factors released from stem cells in an oriented manner, targeted repair and therapy effects of stem cells can be improved remarkably, a physical barrier is formed by gel so that stem cell tumor formation risks are avoided, and by the structures of the microspheres, the cell factors released from the stem cells have the characteristic of controllability in slow releasing, and thus, the stem cell microsphere gel compound conforms to development tendency of safe drug administration and accurate drug administration, and has high clinical application value.

Description

technical field [0001] The invention relates to the fields of stem cells and biological materials, more particularly to a stem cell microsphere gel compound for subcutaneous injection and its application. Background technique [0002] Stem cells are cells with self-replication and differentiation potential, mostly round or oval, small in size, less in cytoplasm, and large in nuclear / cytoplasmic ratio. Stem cells proliferate slowly and have good self-stability, which is conducive to self-replication and maintenance. Due to the slow proliferation, stem cells have sufficient time to find and correct replication errors, avoid self-mutation, and steadily enter a specific differentiation program. Once the body needs it, stem cells can enter a differentiated state, at which time the proliferation rate begins to gradually increase to meet the needs of tissue and organ growth, development and repair. [0003] Since stem cells have the potential of self-replication and differentiati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/38A61L27/50A61L27/52A61L27/58
CPCA61L27/20A61L27/3834A61L27/50A61L27/52A61L27/58A61L2400/06C08L5/08C08L5/04
Inventor 谢海涛郭东升易科青薛卫巍都兴洋
Owner 广东克瑞斯普生物科技有限公司
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