Stem cell microsphere gel compound for subcutaneous injection and application of stem cell microsphere gel compound

Abstract

The invention relates to a stem cell microsphere gel compound for subcutaneous injection and a preparation method and application of the stem cell microsphere gel compound. The preparation method comprises the following specific steps: 1, separating umbilical cord mesenchymal stem cells; 2, preparing stem cell-alginate microspheres; 3, preparing hydrogel; 4, mixing the stem cell-alginate microspheres with the hydrogel, wherein the dispersion concentration of the stem cells in the hydrogen is 105-106 per mL; and 5, subcutaneously injecting the stem cell microsphere gel compound to a wound in atissue to repair tissue injury. A high polymer material such as hyaluronic acid and stem cells are embedded and combined through microspheres, the microspheres are dispersed into gel to form a biological scaffold material with a function of fixing cell factors released from stem cells in an oriented manner, targeted repair and therapy effects of stem cells can be improved remarkably, a physical barrier is formed by gel so that stem cell tumor formation risks are avoided, and by the structures of the microspheres, the cell factors released from the stem cells have the characteristic of controllability in slow releasing, and thus, the stem cell microsphere gel compound conforms to development tendency of safe drug administration and accurate drug administration, and has high clinical application value.

Description

technical field [0001] The invention relates to the fields of stem cells and biological materials, more particularly to a stem cell microsphere gel compound for subcutaneous injection and its application. Background technique [0002] Stem cells are cells with self-replication and differentiation potential, mostly round or oval, small in size, less in cytoplasm, and large in nuclear / cytoplasmic ratio. Stem cells proliferate slowly and have good self-stability, which is conducive to self-replication and maintenance. Due to the slow proliferation, stem cells have sufficient time to find and correct replication errors, avoid self-mutation, and steadily enter a specific differentiation program. Once the body needs it, stem cells can enter a differentiated state, at which time the proliferation rate begins to gradually increase to meet the needs of tissue and organ growth, development and repair. [0003] Since stem cells have the potential of self-replication and differentiati...

AI Technical Summary

Problems solved by technology

However, there are still some shortcomings, which are manifested as the uncontrollable concentration of secreted cytokines and the instability of gel material degradation.