Paecilonyces variotii strain and application thereof

A technology of Paecilomyces variotii and strains, applied to formaldehyde degrading bacteria, Paecilomyces variotii H6 and its application field in degrading formaldehyde, can solve problems such as insufficient database of formaldehyde degrading bacteria, and achieve excellent ability of degrading formaldehyde , the effect of wide distribution and rapid reproduction

Active Publication Date: 2011-04-20
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, with the ever-changing demand for formaldehyde-polluted biological treatment engineering technology, the above-mentioned formaldehyde-degrading bacteria database is very

Method used

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  • Paecilonyces variotii strain and application thereof
  • Paecilonyces variotii strain and application thereof
  • Paecilonyces variotii strain and application thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0026] Example 1 Isolation and identification of formaldehyde-degrading bacterium of the present invention, Paecilomyces variotii (Paecilomyces variotii) H6

[0027] 1. Medium formula

[0028] Basic medium: 1.0% (w·v -1 ) Glucose, 0.2% (w·v -1 )NaNO 3 , 0.1% (w·v -1 )K 2 HPO 4 , 0.05% (w·v -1 )MgSO 4 ·7H 2 O, 0.05% (w·v -1 )KCl, 0.001%(w·v -1 )FeSO 4 ·7H 2 O, 0.01% (w·v -1 ) Yeast extract, 0.1% (w·v -1 ) Formaldehyde (commercially available formaldehyde standard solution for formaldehyde), the final pH of the medium is 6.0. (If there is no special instructions, the following basic medium is the formula medium)

[0029] Cha's medium formula: 3.0% (w·v -1 ) Sucrose, 0.3% (w·v -1 )NaNO 3 , 0.1% (w·v -1 )K 2 HPO 4 , 0.05% (w·v -1 )MgSO 4 ·7H 2 O, 0.05% (w·v -1 )KCl, 0.001%(w·v -1 )FeSO 4 ·7H 2 O, 1.5~2.0% (w·v -1 ) Agar.

[0030] Wort medium formula: 2.0% (w·v -1 ) Malt extract, 0.1% (w·v -1 )Peptone, 2.0% (w·v -1 )Glucose, 1.5~2.0%(w·v -1 ) Agar.

[0031] Cha's Yeast Extract Agar Formula:...

Example Embodiment

[0040] Example 2 Degradation experiment of formaldehyde by Paecilomyces variotii H6 of the present invention

[0041] 1. Production of first-class seeds

[0042] Select Paecilomyces variotii H6 with good sporulation and inoculate it in minimal medium, shaker 160r·min -1 ,Incubate at 30℃ for 2~3d, when the mold enters the rapid growth phase, determine the OD regularly 475 Value when OD 475 When the value rises rapidly, this bacterial solution can be used as a first-level seed.

[0043] 2. Experimental program

[0044] Choose a large-mouthed 150mL Erlenmeyer flask to prepare basic medium, C (formaldehyde) Press about 0.1%, 0.15%, 0.2%, 0.25% (w·v -1 ) Join (C formaldehyde Based on the actual measurement data), the bottle mouth is sealed with a sterile sealing culture film, and the first-level seed (OD 475 The value is 0.915), except for the blank, each bottle is connected to 5mL, and placed on a shaker at 30℃160r·min -1 Incubate and sample at 0, 24, 48, 72, 96, 120, and 144 hours of ino...

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Abstract

The invention discloses a paecilonyces variotii strain H6 and application thereof. The paecilonyces variotii strain H6 with a preservation number of CGMCC No. 3722 is preserved at China General Microbiological Culture Collection Center on April 9, 2010. The paecilonyces variotii strain H6 widely spreads in the nature, quickly propagates and has strong growth capability. The invention provides the application of the paecilonyces variotii strain H6 in formaldehyde degradation and provides the technical support for the biological treatment engineering with formaldehyde pollution.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a formaldehyde-degrading bacterium, in particular to Paecilomyces variotii H6 (Paecilomyces variotii) and its application in degrading formaldehyde. Background technique [0002] Due to the wide range and importance of the application field, studies on the formaldehyde degradation ability of formaldehyde-degrading bacteria have been reported for a long time. [0003] In the early days, people began to study the degradation of formaldehyde at low concentrations, and in recent years, there have been reports on the degradation of formaldehyde at high concentrations. Pseudomonas putida A2 isolated by Adroer et al. (1990) can degrade 250mg L of formaldehyde -1 ; Halomonas sp.MAC isolated by Azachi1 et al. (1995) can only tolerate formaldehyde 75-100mg L -1 ; Doronina et al. (1997) isolated Pseudomonas.alcaligenes can degrade formaldehyde 200mg L -1 , strain Pseudomonas putida J3 can degr...

Claims

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Application Information

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IPC IPC(8): C12N1/14A62D3/02C12R1/79A62D101/28
Inventor 陈能场黄赛花卢普相彭珏孙翠香高原雪刘婷琳
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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