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Paecilonyces variotii strain and application thereof

A technology of Paecilomyces variotii and strains, applied in Paecilomyces variotii H6 and its application in degrading formaldehyde, in the field of formaldehyde degrading bacteria, can solve problems such as insufficient database of formaldehyde degrading bacteria, and achieve excellent ability to degrade formaldehyde , strong growth ability, rapid reproduction effect

Active Publication Date: 2012-07-25
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, with the ever-changing demand for formaldehyde-polluted biological treatment engineering technology, the above-mentioned formaldehyde-degrading bacteria database is very insufficient, and more technical research results on formaldehyde-degrading bacteria are urgently needed to provide strong help for formaldehyde-polluted biological treatment engineering

Method used

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  • Paecilonyces variotii strain and application thereof
  • Paecilonyces variotii strain and application thereof
  • Paecilonyces variotii strain and application thereof

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Effect test

Embodiment 1

[0026] Embodiment 1 Isolation and identification of formaldehyde-degrading bacteria of the present invention-Paecilomyces variotii (Paecilomyces variotii) H6

[0027] 1. Culture medium formula

[0028] Basic medium: 1.0% (w·v -1 ) Glucose, 0.2% (w·v -1 )NaNO 3 , 0.1% (w·v -1 )K 2 HPO 4 , 0.05% (w·v -1 )MgSO 4 ·7H 2 O, 0.05% (w·v -1 ) KCl, 0.001% (w·v -1 )FeSO 4 ·7H 2 O, 0.01% (w·v -1 ) yeast extract, 0.1% (w·v -1 ) formaldehyde (commercially available formaldehyde standard solution is used for formaldehyde), and the final pH of the culture medium is 6.0. (Unless otherwise specified, the basic medium below is this formula medium)

[0029] Cha's medium formula: 3.0% (w·v -1 ) sucrose, 0.3% (w·v -1 )NaNO 3 , 0.1% (w·v -1 )K 2 HPO 4 , 0.05% (w·v-1 )MgSO 4 ·7H 2 O, 0.05% (w·v -1 ) KCl, 0.001% (w·v -1 )FeSO 4 ·7H 2 O, 1.5~2.0% (w·v -1 ) agar.

[0030] Wort medium formula: 2.0% (w v -1 ) malt extract, 0.1% (w·v -1 ) peptone, 2.0% (w·v -1 ) Glucose, 1.5...

Embodiment 2

[0040] Embodiment 2 The degradation experiment of Paecilomyces variotii (Paecilomyces variotii) H6 of the present invention to formaldehyde

[0041] 1. Production of primary seeds

[0042] Paecilomyces variotii H6 with good sporulation was selected and inoculated in the basic medium, and the shaking table was 160r min -1 , culture at 30°C for 2-3 days, when the mold enters the rapid growth period, measure the OD regularly 475 value, when OD 475 When the value rises rapidly, the bacterial solution can be used as a first-class seed.

[0043] 2. Experimental plan

[0044] Select a large-mouth 150mL Erlenmeyer flask to prepare basic medium, C (甲醛) According to about 0.1%, 0.15%, 0.2%, 0.25% (w·v -1 ) to join (C 甲醛 The actual measurement data shall prevail), the bottle mouth shall be sealed with aseptic sealing culture film, and the first-grade seeds shall be cultivated (OD 475 Value is 0.915), add 5mL to each bottle except the blank, place on a shaker at 30°C 160r·min -1 C...

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Abstract

The invention discloses a paecilonyces variotii strain H6 and application thereof. The paecilonyces variotii strain H6 with a preservation number of CGMCC No. 3722 is preserved at China General Microbiological Culture Collection Center on April 9, 2010. The paecilonyces variotii strain H6 widely spreads in the nature, quickly propagates and has strong growth capability. The invention provides theapplication of the paecilonyces variotii strain H6 in formaldehyde degradation and provides the technical support for the biological treatment engineering with formaldehyde pollution.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a formaldehyde-degrading bacterium, in particular to Paecilomyces variotii H6 (Paecilomyces variotii) and its application in degrading formaldehyde. Background technique [0002] Due to the wide range and importance of the application field, studies on the formaldehyde degradation ability of formaldehyde-degrading bacteria have been reported for a long time. [0003] In the early days, people began to study the degradation of formaldehyde at low concentrations, and in recent years, there have been reports on the degradation of formaldehyde at high concentrations. Pseudomonas putida A2 isolated by Adroer et al. (1990) can degrade 250mg L of formaldehyde -1 ; Halomonas sp.MAC isolated by Azachi1 et al. (1995) can only tolerate formaldehyde 75-100mg L -1 ; Doronina et al. (1997) isolated Pseudomonas.alcaligenes can degrade formaldehyde 200mg L -1 , strain Pseudomonas putida J3 can degr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/79A62D101/28
Inventor 陈能场黄赛花卢普相彭珏孙翠香高原雪刘婷琳
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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