Halictidae aspergillus nomius strain and application thereof

A technology of Aspergillus melida and strains, applied in Aspergillus melida H5 and its application in degrading formaldehyde, in the field of formaldehyde-degrading bacteria, can solve problems such as insufficient database of formaldehyde-degrading bacteria, and achieve wide distribution, rapid reproduction and strong growth ability. Effect

Active Publication Date: 2012-07-25
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, with the ever-changing demand for formaldehyde-polluted biological treatment engineering technology, the above-mentioned formaldehyde-degrading bacteria database is very insufficient, and more technical research results on formaldehyde-degrading bacteria are urgently needed to provide strong help for formaldehyde-polluted biological treatment engineering

Method used

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  • Halictidae aspergillus nomius strain and application thereof
  • Halictidae aspergillus nomius strain and application thereof
  • Halictidae aspergillus nomius strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Isolation and identification of formaldehyde-degrading bacteria of the present invention-Aspergillus nomius (Aspergillus nomius) H5

[0027] 1. Culture medium formula

[0028] Basic medium: 1.0% (w·v -1 ) Glucose, 0.2% (w·v -1 )NaNO 3 , 0.1% (w·v -1 )K 2 HPO 4 , 0.05% (w·v -1 )MgSO 4 ·7H 2 O, 0.05% (w·v -1 ) KCl, 0.001% (w·v -1 )FeSO 4 ·7H 2 O, 0.01% (w·v -1 ) yeast extract, 0.1% (w·v -1 ) formaldehyde (commercially available formaldehyde standard solution is used for formaldehyde), and the final pH of the culture medium is 6.0. (Unless otherwise specified, the basic medium below is this formula medium)

[0029] Cha's medium formula: 3.0% (w·v -1 ) sucrose, 0.3% (w·v -1 )NaNO 3 , 0.1% (w·v -1 )K 2 HPO 4 , 0.05% (w·v -1 )MgSO 4 ·7H 2 O, 0.05% (w·v -1) KCl, 0.001% (w·v -1 )FeSO 4 ·7H 2 O, 1.5~2.0% (w·v -1 ) agar.

[0030] Wort medium formula: 2.0% (w v -1 ) malt extract, 0.1% (w·v -1 ) peptone, 2.0% (w·v -1 ) Glucose, 1.5~2.0% (w...

Embodiment 2

[0040] Embodiment 2 The present invention collects Aspergillus nomius (Aspergillus nomius) H5 to the degradation experiment of formaldehyde

[0041] 1. Production of primary seeds

[0042] Select Aspergillus nomius H5 with good spore production, inoculate it in the basic medium, and shake it at 160r min -1 , culture at 30°C for 2-3 days, when the mold enters the rapid growth period, measure the OD regularly 475 value, when OD 475 When the value rises rapidly, the bacterial solution can be used as a first-class seed.

[0043] 2. Experimental plan

[0044] Select a large-mouth 150mL Erlenmeyer flask to prepare basic medium, C (甲醛) According to about 0.1%, 0.15%, 0.2%, 0.25% (w·v -1 ) to join (C 甲醛 The actual measurement data shall prevail), the bottle mouth shall be sealed with aseptic sealing culture film, and the first-grade seeds shall be cultivated (OD 475 Value is 0.878), add 5mL to each bottle except the blank, place on a shaker at 30°C 160r·min -1 Cultivate, and t...

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Abstract

The invention discloses a halictidae aspergillus nomius strain H5 and application thereof. The halictidae aspergillus nomius strain H5 is preserved in China general microbiological culture collection in April 9th, 2010 with the preservation number of CGMCCNo.3721, is widely distributed in the natural world, can be quickly bred and has strong growing ability. The application of the halictidae aspergillus nomius strain H5 in degrading formaldehyde provides technical support for biological treatment engineering about formaldehyde pollution.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a formaldehyde-degrading bacterium, in particular to an Aspergillus nomius H5 and its application in degrading formaldehyde. Background technique [0002] Due to the wide range and importance of the application field, studies on the formaldehyde degradation ability of formaldehyde-degrading bacteria have been reported for a long time. [0003] In the early days, people began to study the degradation of formaldehyde at low concentrations, and in recent years, there have been reports on the degradation of formaldehyde at high concentrations. Pseudomonas putida A2 isolated by Adroer et al. (1990) can degrade 250mg L of formaldehyde -1 ; Halomonas sp.MAC isolated by Azachi1 et al. (1995) can only tolerate formaldehyde 75-100mg L -1 ; Doronina et al. (1997) isolated Pseudomonas.alcaligenes can degrade formaldehyde 200mg L -1 , strain Pseudomonas putida J3 can degrade formaldehyde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/66A62D101/28
Inventor 黄赛花陈能场卢普相刘婷琳张朝阳杨孟娟
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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