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Pseudomonas hibiscicola capable of degrading tetracycline pollutants in water and application thereof

A technology of Pseudomonas and tetracycline, applied in the biological field, can solve the problems of incomplete absorption of antibiotics, soil pollution, etc., and achieve the effects of good tetracycline degradation ability, strong growth ability, and strong application value

Active Publication Date: 2015-03-11
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology

Tetracycline antibiotics are currently one of the most widely used antibiotics in the world. In recent years, tetracycline antibiotics have been widely used as veterinary drugs and feed additives in livestock, poultry and aquaculture. However, these antibiotics are not fully absorbed. , most of which are excreted in the form of original drugs and metabolites, 80% of which are applied to farmland without comprehensive treatment, causing soil pollution

Method used

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  • Pseudomonas hibiscicola capable of degrading tetracycline pollutants in water and application thereof
  • Pseudomonas hibiscicola capable of degrading tetracycline pollutants in water and application thereof

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Embodiment 1

[0014] The screening of embodiment 1 Pseudomonas hibiscus DT1:

[0015] (1) Material preparation:

[0016] 1. The experimental soil is simulated tetracycline-contaminated soil, which was obtained from China University of Geosciences.

[0017] 2. Culture medium:

[0018] Screening medium: 10g / L peptone, 5g / L sodium chloride, 0.5g / L dipotassium hydrogen phosphate, 1.5g / L agar, pH 7.

[0019] Liquid enrichment medium: 10g / L peptone, 5g / L sodium chloride, 0.5g / L dipotassium hydrogen phosphate, pH 7.

[0020] LB liquid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, pH7.0.

[0021] LB solid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, agar 15g / L (solid), pH7.0.

[0022] LB slant medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, agar 20g / L (solid), pH7.0.

[0023] 3. Experimental instruments and equipment:

[0024] Guohua SHA-B consta...

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to pseudomonas hibiscicola capable of degrading tetracycline pollutants in water and application thereof. The pseudomonas hibiscicola DT1 is preserved in China center for type culture collection with the preservation number of CCTCCNO.M2014244. The pseudomonas hibiscicola DT1 has stronger growth capability and good tetracycline degradation capability in the environment containing the tetracycline, the strain can effectively remove the tetracycline in wastewater within 72 hours, and the removal efficiency reaches 83.21wt%. The pseudomonas hibiscicola provides a useful source of bacteria for degradation of tetracycline organic pollutants, the function aspect application of pseudomonas hibiscicola is broadened, and the pseudomonas hibiscicola has strong application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pseudomonas hibiscus that degrades tetracycline pollutants in water bodies and an application thereof. Background technique [0002] Tetracycline antibiotics are a class of broad-spectrum antibiotics produced by actinomycetes, and their structures all contain naphthacene basic skeletons. Tetracycline antibiotics are currently one of the most widely used antibiotics in the world. In recent years, tetracycline antibiotics have been widely used as veterinary drugs and feed additives in livestock, poultry and aquaculture. However, these antibiotics are not fully absorbed. , most of which are eliminated in the form of original drugs and metabolites, and 80% of the feces are applied to farmland without comprehensive treatment, causing soil pollution. After tetracycline antibiotics enter the soil environment, they exist in the soil environment for a long time through adsorpti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/38
Inventor 冷一非鲍建国
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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