Stenotrophomonas maltophilia DS4

A technology of Stenotrophomonas and maltophilia, applied in the direction of bacteria, microorganisms, water pollutants, etc., can solve the problem of strains of organic pollutants without microbial degradation methods, and achieve good removal of organic pollutants in saponin wastewater ability, widening application, and the effect of strong growth ability

Inactive Publication Date: 2013-07-31
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, physical or chemical methods are mostly used in the treatment of saponin wastewater. In the prior art, there are no reports of microbial degradation methods and bacterial strains for degrading saponin wastewater organic pollutants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The screening method of embodiment 1 Stenotrophomonas maltophilia DS4

[0012] (1) Material preparation:

[0013] 1. The experimental wastewater and pool bottom sludge were taken from the biochemical treatment system of a saponin production plant in Shiyan City, Hubei Province.

[0014] 2. Culture medium:

[0015] Separation medium: K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, NH 4 C1 1.0g / L, CaCl 2 0.01g / L, KH 2 PO 4 0.5g / L, glucose 0.5g / L, pH 7.0.

[0016] Screening medium: K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, NH 4 C1 1.0g / L, CaCl 2 0.01g / L, KH 2 PO 4 0.5g / L, dissolved in 1L saponin wastewater, pH 7.0.

[0017] LB liquid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, pH7.0.

[0018] LB solid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, agar 15g / L (solid), pH7.0.

[0019] LB slant medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L,...

Embodiment 2

[0033] Example 2 Application of Stenotrophomonas maltophilia DS4

[0034] A single colony of Stenotrophomonas maltophilia DS4 was picked and cultured overnight in LB solid medium. According to the cultured Stenotrophomonas maltophilia DS4 and saponin wastewater (that is, the experimental wastewater, it was taken from a certain site in Shiyan City, Hubei Province). The biochemical treatment system for wastewater discharged from a saponin production plant) is inoculated at a volume ratio of 0.5-2:100, cultivated at a constant temperature of 30-35°C, with a pH value of 6-8, and reacted for 48-72 hours.

[0035] The LB solid medium is: 10.0 g of peptone, 5.0 g of yeast extract, 10.0 g of NaCl, 1000 mL of distilled water, 15 g of agar (solid), pH 7.0.

[0036] The degradation efficiency of the screened microorganisms to organic pollutants in saponin wastewater was measured.

[0037] Pick a single colony and culture overnight in the isolation medium, take 1 mL of the bacterial solu...

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PUM

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Abstract

The invention discloses Stenotrophomonas maltophilia DS4. The Stenotrophomonas maltophilia DS4 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M2010147. The Stenotrophomonas maltophilia DS4 has a strong growing ability and a good ability of removing organic pollutants from saponin wastewater in high-concentration sulfate ion environments; and the strain can remove chemical oxygen demand (COD) at the concentration of 2,930mg / L from the saponin wastewater within 72 hours, and the removal efficiency reaches 62.34 percent. The invention provides auseful bacterial source for degrading high-concentration organic pollutants in the saponin wastewater, broadens the application of functions of Stenotrophomonas maltophilia, and has relatively high application value.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to Stenotrophomonas maltophilia DS4. Background technique [0002] The wastewater produced during the production of saponin (such as diosgenin) has high chroma, high acidity, high concentration of organic pollutants, high chemical oxygen demand (COD), and poor biodegradability. It is a very difficult wastewater to treat. "Saponin Industrial Water Pollutant Discharge Standard" (GB 20425-2006) stipulates that COD emissions should be less than 300mg / L since 2009. However, the current COD concentration of saponin hydrolysis stock solution reaches 20000-40000mg / L The COD concentration of wastewater is 4000-12000mg / L. If it is directly discharged into the water body, the concentration of organic pollutants in the water body will increase significantly, which will cause serious pollution of rivers and lakes, which not only directly affects people's living environment, but also causes economic dam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01C02F101/34
Inventor 郑丹鲍建国
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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