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Silage fermentation accelerator and application thereof

A fermentation accelerator and silage technology, applied in the application, animal feed, animal husbandry and other directions, can solve the problems of different formulations of silage fermentation accelerators, and achieve the effects of simple and easy preparation, improved meat quality and strong practicability

Inactive Publication Date: 2015-11-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at problems such as different formulas of silage fermentation accelerators, the object of the present invention is to provide a kind of silage fermentation accelerator and its application

Method used

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  • Silage fermentation accelerator and application thereof
  • Silage fermentation accelerator and application thereof
  • Silage fermentation accelerator and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: This silage fermentation promotion agent preparation method is:

[0021] ① Save the strain of the Bacterium Bacterium in the sliced bacteria to the MRS liquid medium, and settle for 16h at 37 ° C. 11 CFU / ml, use it as a lactic acid bacteria fermentation liquid;

[0022] ② Inoculate the plant lactobacillus saved by the slope to the MRS liquid medium, settle for 16h at 37 ° C, so that the effective live bacteria in the final fermentation liquid ≥10 ≥10 11 CFU / ml;

[0023] ③ Inoculate the strain of Berbalbalbalbalbalbalbalbuliniberi saved in the slope to the LB liquid medium, it is cultivated for 16H at 37 ° C and 150rpm shake in bed, so that the effective live bacteria in the final fermentation solution ≥10 10 CFU / ml, which generates bacterial liquid as a cellulose enzyme;

[0024] ④ The fermentation liquid of Tomosaccharide, Plant Lactobacillus and Bacteria Bacillus Bacteria is prepared in the following component:

[0025] Bacterium A: Totogliococcus and Bacteria ...

Embodiment 2

[0049] Example 2: This silage fermentation promotional agent consists of effective live bacteria ≥10 11 CFU / ML's Tibetan bacterial bacteria and effective live bacteria ≥10 10 CFU / ML's Berbus Bacillus Bacillus liquid is made by mixed with a volume ratio of 2: 1.5.

[0050] The preparation method of the above silage fermentation promotion agent is:

[0051] ① Save the strain of the Bacterium Bacterium in the sliced bacteria into the MRS liquid medium, and set the cultivation of 18h at 37 ° C to make the effective live bacteria in the final fermentation liquid ≥10 ≥10 11 CFU / ml, use it as a lactic acid bacteria fermentation liquid;

[0052] ② Inoculate the strain of Berbalbalbalbalbalbalbalbuliniberi saved in the slope to the LB liquid medium, it is cultivated for 20 hours of 37 ° C and 150rpm shake in bed, so that the effective live bacteria in the final fermentation solution ≥10 10 CFU / ml, which generates bacterial liquid as a cellulose enzyme;

[0053] ③ The fermentation solution ...

Embodiment 3

[0056] Example 3: This silage fermentation promotional agent consists of effective live bacteria ≥10 11 CFU / ML's Tibetan bacterial bacteria and effective live bacteria ≥10 10 CFU / ML's Berbalobacterium Bacteria Bacillus liquid is made by mixed with a volume ratio of 1.5: 1.2.

[0057] The preparation method of the above silage fermentation promotion agent is:

[0058] ① Save the strain of the Bacterium Bacterium in the sliced bacterium to the MRS liquid medium, and then retract for 20 hours at 37 ° C, so that the effective live bacteria in the final fermentation liquid ≥10 ≥10 11 CFU / ml, use it as a lactic acid bacteria fermentation liquid;

[0059] ② Inoculate the strain of Berbalbalbalbalbalbalbalbuliniberi saved in the slope to the LB liquid medium, 37 ° C and 150rpm shake the bed for 18h, so that the effective live bacteria in the final fermentation solution ≥10 10 CFU / ml, which generates bacterial liquid as a cellulose enzyme;

[0060] ③ The fermentation solution of Tibetan ba...

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Abstract

The invention discloses a silage fermentation accelerator. The accelerator is prepared by mixing pediococcus pentosaceus bacterial liquid having an effective viable count of more than or equal to 10<11>CFU / mL and bacillus subtilis bacterial liquid having an effective viable count of more than or equal to 10<10> CFU / mL in a volume ratio of (1-2):(1-1.5). The silage fermentation accelerator is applied to corn straw coating, bundling and silage fermentation, a method of spraying and adding bacterial liquid is adopted, and the dosage of the bacterial liquid is 4L / ton. According to the method, corn straws are rubbed by utilizing a straw rubbing machine, are spread and aired until corn straws contain proper moisture, are sprayed with the silage bacterial liquid, are uniformly mixed, are coated and bundled by means of a coating machine, are fermented for about 30 days and are unpacked for use. The production method is simple and feasible, and has the advantages of high efficiency, strong practicality and high economic value.

Description

Technical field [0001] The invention involves a silage fermentation promotion agent and its application. Background technique [0002] With the rapid development of my country's animal husbandry industry and the continuous adjustment of planting structure, the gradual implementation of national ecological construction planning, many regions will increase the proportion of herbivorous livestock breeding in the breeding industry as an important measure to adjust the agricultural structure. The year -on -year increase has caused the supply of forage to lose in the spring and winter. It is urgently needed to reserve a certain amount of forage to regulate the balancing of the local grass all year round, in order to help the sustainable development of the local animal husbandry industry.How to effectively make green and green feed a balanced supply throughout the year has become a hot issue in rural large -scale farming, and silage is the best way to solve this contradiction. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K3/02
Inventor 林连兵柳佳志邓先余魏云林张琦季秀玲
Owner KUNMING UNIV OF SCI & TECH
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