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Application of Bacillus cereus DS1 in degradation of organic pollutants in saponin waste water

A technology of Bacillus cereus and organic pollutants, applied in the biological field, can solve the problem of strains without microbial degradation methods of organic pollutants, etc., and achieve the effects of good organic pollutant ability, wide application and strong growth ability.

Inactive Publication Date: 2013-04-03
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] At present, physical or chemical methods are mostly used in the treatment of saponin wastewater. In the prior art, there are no reports of microbial degradation methods and bacterial strains for degrading saponin wastewater organic pollutants.

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  • Application of Bacillus cereus DS1 in degradation of organic pollutants in saponin waste water

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with the accompanying drawings and examples, but of course the following examples should not be construed as limiting the present invention.

[0015] 1. Screening of Bacillus cereus DS1:

[0016] (1) Material preparation:

[0017] 1. The experimental wastewater and pool bottom sludge were taken from the biochemical treatment system of a turmeric saponin production plant in Shiyan City, Hubei Province.

[0018] 2. Culture medium:

[0019] Screening medium: 3g / L beef extract, 10g / L peptone, 5g / L sodium chloride, 20g / L agar, pH6.8.

[0020] Enrichment medium (g / L): 30g / L dipotassium hydrogen phosphate, 100mg / L anhydrous magnesium sulfate, 10g / L potassium dihydrogen phosphate, 10mg / L manganese sulfate tetrahydrate, 5g / L ammonium nitrate, 10mg / L L ferrous sulfate heptahydrate, 1g / L sodium sulfate, 5mg / L calcium chloride, stored in refrigerator. When in use, dilute 10 times, add 10% glucose, and the pH v...

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Abstract

The invention provides application of a Bacillus cereus DS1 in degradation of organic pollutants in saponin waste water. The Bacillus cereus DS1 is collected at China Center for Type Culture Collection, and the collection number is CCTCC NO:M2010145. The Bacillus cereus DS1 has high growing capability and favorable capability of removing organic pollutants in saponin waste water in the presence of high-concentration sulfate ions. The strain can remove COD (chemical oxygen demand) in saponin waste water by 2550 mg / L within 72 hours, and the removal efficiency is up to 60.14%. The invention provides a useful bacterium source for the degradation of high-concentration organic pollutants in saponin waste water, and widens the functional application of Bacillus cereus, thereby having high application values.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a bacillus cereus ( Bacillus Cereus ) Application of DS1 in degrading organic pollutants in saponin wastewater. Background technique [0002] Saponin (such as diosgenin) production wastewater has the characteristics of small wastewater volume, large chroma, high concentration of organic matter, high acidity, high temperature, etc., and poor biodegradability, which is a very difficult wastewater to treat. [0003] According to the "Saponin Industrial Water Pollutant Discharge Standard" (GB20425-2006), the COD emission should be less than 300mg / L since 2009. At present, the COD concentration of saponin hydrolysis stock solution reaches about 20000-40000mg / L, and the COD concentration of comprehensive wastewater is about 4000-12000mg / L. If it is directly discharged into the water body, the concentration of organic pollutants in the water body will increase significantly, which will ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/30
Inventor 郑丹鲍建国
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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