Radiation-resistant type paenibacillus sp. KH9 and application thereof in biological antitranspirant
A bacillus, radiation-resistant technology, applied in the direction of microorganism-based methods, chemicals for biological control, applications, etc., can solve the problems of reducing plant transpiration, achieve enhanced photosynthesis, reduce damage, and produce polysaccharides high effect
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Embodiment 1
[0022] Example 1: Isolation, screening and identification of Paenibacillus sp. KH9CGMCC No.12799
[0023] 1. Isolation and screening of strains
[0024] Paenibacillus sp. KH9 used in the present invention is sampled from a large amount of soil collected from the arid desert area around Lop Nur, Xinjiang by the Institute of Microbiology Application of Xinjiang Academy of Agricultural Sciences, and is separated after irradiation by a 5000KGy cobalt source. The polysaccharide-producing bacteria in the soil layer were isolated by the method, and the bacterial strains were purified by the plate streaking method. After optimization and screening, a batch of bacterial strains with good growth and strong polysaccharide-producing ability were selected, and a strain numbered KH9 was selected from them.
[0025] Separation steps:
[0026] (1) Preparation of soil sample dilution: dissolve the soil sample irradiated by 5000KGy cobalt source in a triangular flask containing sterile water a...
Embodiment 2
[0037] Example 2: Molecular level identification of Paenibacillus sp. KH9CGMCC No.12799
[0038] The total DNA of the strain KH9 was extracted, and the general primers for PCR amplification using bacterial 16S rDNA were used for PCR amplification, and the PCR products were sequenced after being gel-cut and purified. The obtained sequences of the experimental strains were compared with the known sequences in the GenBank database by BLAST to determine the closest species relationship with the experimental strains. Combined with the sequence alignment in EzTaxon (http: / / www.ezbiocloud.net / eztaxon) and retrieve the sequence of related type strains, in order to carry out the phylogenetic tree analysis of the sequence of the standard type strains, the MEGA 5.0 software package was used to adopt the neighbor-joining method (Neighbor- Joining method) for cluster analysis and phylogenetic tree construction. It can be seen from the phylogenetic tree that the maximum homology between th...
Embodiment 3
[0039] Example 3: Preparation of Paenibacillus sp. KH9CGMCC No.12799 cells
[0040] Inoculate the activated bacterial strain of the present invention Paenibacillus sp. (Paenibacillus sp.) KH9CGMCCNo.12799 in the seed test tube that 5ml PDA liquid medium is housed, cultivate at 30 DEG C, after 200rpm vibration culture 36h, press 2% inoculum size Inoculate in PDA liquid medium (filling amount is 100ml PDA liquid medium in a 500ml Erlenmeyer flask), cultivate at 30°C, and ferment in a shaking flask at 200rpm for 3-4 days, and the number of effective viable bacteria is ≥ 200 million / ml.
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