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Radiation-resistant type paenibacillus sp. KH9 and application thereof in biological antitranspirant

A bacillus, radiation-resistant technology, applied in the direction of microorganism-based methods, chemicals for biological control, applications, etc., can solve the problems of reducing plant transpiration, achieve enhanced photosynthesis, reduce damage, and produce polysaccharides high effect

Inactive Publication Date: 2017-11-07
新疆万康诚一肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combining microorganisms and anti-transpiration agents to develop new biological anti-transpiration agents will exert more efficient and reliable effects while retaining their respective advantages. Few, so it is necessary to further explore the drought resistance of microorganisms, and provide resistant strains with reduced plant transpiration and stable performance for microbial drought resistance, which will have potential application development prospects in the field of plant drought resistance

Method used

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  • Radiation-resistant type paenibacillus sp. KH9 and application thereof in biological antitranspirant
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  • Radiation-resistant type paenibacillus sp. KH9 and application thereof in biological antitranspirant

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Experimental program
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Effect test

Embodiment 1

[0022] Example 1: Isolation, screening and identification of Paenibacillus sp. KH9CGMCC No.12799

[0023] 1. Isolation and screening of strains

[0024] Paenibacillus sp. KH9 used in the present invention is sampled from a large amount of soil collected from the arid desert area around Lop Nur, Xinjiang by the Institute of Microbiology Application of Xinjiang Academy of Agricultural Sciences, and is separated after irradiation by a 5000KGy cobalt source. The polysaccharide-producing bacteria in the soil layer were isolated by the method, and the bacterial strains were purified by the plate streaking method. After optimization and screening, a batch of bacterial strains with good growth and strong polysaccharide-producing ability were selected, and a strain numbered KH9 was selected from them.

[0025] Separation steps:

[0026] (1) Preparation of soil sample dilution: dissolve the soil sample irradiated by 5000KGy cobalt source in a triangular flask containing sterile water a...

Embodiment 2

[0037] Example 2: Molecular level identification of Paenibacillus sp. KH9CGMCC No.12799

[0038] The total DNA of the strain KH9 was extracted, and the general primers for PCR amplification using bacterial 16S rDNA were used for PCR amplification, and the PCR products were sequenced after being gel-cut and purified. The obtained sequences of the experimental strains were compared with the known sequences in the GenBank database by BLAST to determine the closest species relationship with the experimental strains. Combined with the sequence alignment in EzTaxon (http: / / www.ezbiocloud.net / eztaxon) and retrieve the sequence of related type strains, in order to carry out the phylogenetic tree analysis of the sequence of the standard type strains, the MEGA 5.0 software package was used to adopt the neighbor-joining method (Neighbor- Joining method) for cluster analysis and phylogenetic tree construction. It can be seen from the phylogenetic tree that the maximum homology between th...

Embodiment 3

[0039] Example 3: Preparation of Paenibacillus sp. KH9CGMCC No.12799 cells

[0040] Inoculate the activated bacterial strain of the present invention Paenibacillus sp. (Paenibacillus sp.) KH9CGMCCNo.12799 in the seed test tube that 5ml PDA liquid medium is housed, cultivate at 30 DEG C, after 200rpm vibration culture 36h, press 2% inoculum size Inoculate in PDA liquid medium (filling amount is 100ml PDA liquid medium in a 500ml Erlenmeyer flask), cultivate at 30°C, and ferment in a shaking flask at 200rpm for 3-4 days, and the number of effective viable bacteria is ≥ 200 million / ml.

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Abstract

The invention discloses radiation-resistant type paenibacillus sp. KH9 and an application thereof in a biological antitranspirant. A soil sample collected in arid desert region around the Lop Nor area in Xinjiang Province is separated, screened and cultured, and the radiation-resistant type paenibacillus sp. KH9 with relatively high polysaccharide production capacity and the function of reducing plant transpiration is obtained and has the CGMCC No.12799. The radiation-resistant type paenibacillus sp. KH9 has remarkable physiological-biochemical characteristic difference and molecular level difference by comparison with common paenibacillus sp.; when the screened-out radiation-resistant type paenibacillus sp. KH9 and the antitranspirant are compounded and used for drought resistance, biological films are formed on leaves of crops, transpiration of the crops can be reduced effectively, the photosynthesis function is improved, the chlorophyll content is increased by 4.50%, and the radiation-resistant type paenibacillus sp. KH9 have higher growth promoting capacity, osmotic adjustment ability and active oxygen defense capacity for crops and can reduce damage to the crops under drought stress.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to the technical field of a new strain of Paenibacillus radioresistant and its application in biological anti-transpiration agents. technical background [0002] With the intensification of the greenhouse effect, the drought trend will continue further. At present, 1 / 3 of the land in the world is in arid or semi-arid state, and 48.9% of the cultivated land in our country is in arid or semi-arid area. Drought stress, as a major abiotic stress, seriously restricts the sustainable development of agriculture, which will lead to slow crop growth, reduced yield, and aggravated pests and diseases. Therefore, it is necessary to develop efficient drought-resistant and water-saving measures. [0003] In recent years, chemical water-saving technology has been more and more applied to agricultural drought resistance, and the use of anti-transpiration agent is one of the more effective mea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 顾美英张志东徐万里唐琦勇刘广成王博唐光木马海刚
Owner 新疆万康诚一肥业有限公司
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