Tissue culture method for increasing survival rate of psidium guajava L.

A technology of pearl guava and tissue culture, applied in the field of plant tissue culture, can solve problems such as easy-to-contaminate bacteria, sensitivity to sterilizers, reduce production costs, etc., and achieve the effects of promoting induction culture, promoting incision healing, and promoting plant growth

Active Publication Date: 2016-09-28
广西陆川县乌坭坡珍珠番石榴专业合作社
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Guava is a plant of the genus Guava in the family Myrtaceae. It is native to tropical America. Now it is widely cultivated in many tropical and subtropical regions. In my country, it is mainly distributed in Hainan, Guangxi, Guangdong, Fujian and Taiwan. The growth of guava fruit trees The requirements on the environment are very strict. The current seedling cultivation methods of guava fruit trees are mostly grafting cultivation. The grafting cultivation period is usually 1-2 years to obtain pearl guava seedlings. The cultivation period of grafting cultivation is long and the cultivation method is complicated. At present, there are also Some scholars have begun to study the tissue culture method of the pearl guava fruit tree, but the explants of the pearl guava fruit tree are easy to be infected with bacteria, cause browning, and are sensitive to sterilizers, so it is not easy to cultivate pearl guava fruit trees according to conventional methods.
[0003] At present, in the process of plant tissue culture, the commonly used sterilizing agent in my country is mainly mercury chloride. A lot of research on the toxic effect of mercury has been carried out at home and abroad, and it has been confirmed that mercury is important for every growth process of cells. Therefore, the explants sterilized by mercury chloride need to completely remove the residual Hg+, so as to eliminate the damage to the explants caused by the trace amount of residual mercury chloride. The explants of pearl guava fruit trees are not easy to carry out tissue culture and reproduction. It is necessary to provide an ideal tissue culture method in the tissue culture process of pearl guava, which can not only be applied to various explants of pearl guava, but also effectively disinfect the surface of the explants without sterilizing the explants. Infringement, but also can effectively reduce production costs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture method for increasing survival rate of psidium guajava L.
  • Tissue culture method for increasing survival rate of psidium guajava L.
  • Tissue culture method for increasing survival rate of psidium guajava L.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Tissue culture steps:

[0026] Spray lime powder on the ground of the culture room with doors and windows closed 12 hours before tissue culture, and spray medical alcohol with a concentration of 75% into the air for sterilization; 1. The terminal buds of fruit trees without disease are explants, cut off the top tip, and cut the terminal buds into 1cm in length and 0.4cm in diameter; the explants are washed with running water for 5min, and after being pulled out, use 75% Soak in alcohol solution for 3 minutes, drain and soak in 8% plant treatment solution for 20 minutes to sterilize; inoculate the sterilized explants in the induction medium and induce culture in the incubator, the culture temperature is At 24°C, use a white LED light to illuminate, the light intensity is 500Lx, and the light time is 12h; the explants that grow new shoots are transferred into the subculture medium and subcultured in the incubator, and the culture temperature is At 24°C, use white LED ...

Embodiment 2

[0042] 1. Tissue culture steps:

[0043] Spray lime powder on the ground of the culture room with doors and windows closed 14 hours before tissue culture, and spray medical alcohol with a concentration of 75% into the air for sterilization; 1. The terminal buds of fruit trees without disease are explants, cut off the top tip, and cut the terminal buds into 2cm in length and 0.8cm in diameter; the explants are washed with running water for 8min, and after being pulled out, use 75% Soak in alcohol solution for 5min, drain and soak in 10% plant treatment solution for sterilization for 25min after draining; inoculate the sterilized explants in induction medium and induce culture in an incubator at a temperature of 26°C, light with white LED lights, the light intensity is 800Lx, and the light time is 16h; the explants that grow new shoots are transferred into the subculture medium and subcultured in the incubator, and the culture temperature is At 26°C, use white LED lights for il...

Embodiment 3

[0052] 1. Tissue culture steps:

[0053] Spray lime powder on the ground of the culture room with doors and windows closed 13 hours before tissue culture, and spray medical alcohol with a concentration of 75% into the air for sterilization; 1. The terminal buds of fruit trees without disease are explants, cut off the top tip, and cut the terminal buds into a length of 1.5cm and a diameter of 0.6cm; the explants are washed with tap water for 6 minutes, and the concentration is 75% after being pulled out. Soak in alcohol solution for 4 minutes, drain and soak in 9% plant treatment liquid for 22 minutes to sterilize; inoculate the sterilized explants in the induction medium and induce culture in the incubator, the culture temperature at 25°C, use white LED lights for illumination, the illumination intensity is 600Lx, and the illumination time is 14h; the explants that grow new shoots are transferred into the subculture medium and subcultured in the incubator. at 25°C, use white ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of plant tissue culture and discloses a tissue culture method for increasing the survival rate of psidium guajava L.. The tissue culture method comprises the following first step of disinfection of a culture room, the second step of selection of explants, the third step of sterilization of the explants, the fourth step of initial induction culture, the fifth step of subculture, and the sixth step of seedling hardening. According to the method, the explants are disinfected with plant treating liquid, and thus the survival rate of the explants in the tissue culture process is increased; meanwhile, a commonly-used MS culture medium is replaced with cassava alcohol waste liquid, and thus the tissue culture cost can be effectively reduced. The tissue culture method can be applied to various explants of psidium guajava L., the surfaces of the explants can be effectively disinfected without disoperation to the explants, and meanwhile the production cost can be effectively reduced.

Description

【Technical field】 [0001] The invention relates to the field of plant tissue culture, in particular to a tissue culture method for improving the survival rate of pearl guava. 【Background technique】 [0002] Guava is a plant of the genus Guava in the family Myrtaceae. It is native to tropical America. Now it is widely cultivated in many tropical and subtropical regions. In my country, it is mainly distributed in Hainan, Guangxi, Guangdong, Fujian and Taiwan. The growth of guava fruit trees The requirements on the environment are very strict. The current seedling cultivation methods of guava fruit trees are mostly grafting cultivation. The grafting cultivation period is usually 1-2 years to obtain pearl guava seedlings. The cultivation period of grafting cultivation is long and the cultivation method is complicated. At present, there are also Some scholars begin to study the tissue culture method of pearl guava fruit tree, but the explant of pearl guava fruit tree is easy to infe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 朱万勇
Owner 广西陆川县乌坭坡珍珠番石榴专业合作社
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap