Lactobacillus plantarum strain and application thereof

A technology of Lactobacillus plantarum and strains, which is applied in the direction of bacteria, microorganisms, and preservation of meat/fish with acid, etc., can solve the problems of complex microbial strains, achieve the effects of inhibiting growth and reproduction, reducing inactivation rate, and simple preparation process

Active Publication Date: 2010-06-09
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 38 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the traditional Chinese sausage is a naturally ferm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus plantarum strain and application thereof
  • Lactobacillus plantarum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] Screening and preservation of strains

[0019] This strain is a strain that does not produce amines isolated from traditional Chinese sausages. The culture medium of the isolated strain is centrifuged, the supernatant is taken, and the inhibitory ability of the supernatant to five amine-producing bacteria is observed. And it was added to the medium containing biogenic amine, and the ability of oxidation to reduce biogenic amine was detected.

[0020] The bacteria are Gram-positive bacteria, white colonies, straight rods, short chains, aflagellate, homozygous fermentation, no gas production from glucose fermentation, acetic acid and lactic acid when fermented ribose, no reduction of nitrite, no indole, Negative for hydrogen sulfide production and negative for coagulase; the strain is rod-shaped under ordinary microscopes (see figure 1 ).

[0021] The strain was named Lactobacillus plantarum xlbf001 according to the comparison results of Gen bank, an...

Example Embodiment

[0022] Example 2

[0023] Inhibitory effect of Lactobacillus plantarum xlbf001 on amine-producing bacteria

[0024] Experimental amine-producing bacteria: Enterococcus faecium

[0025] Medium: MRS agar medium (peptone 10g, beef extract 10g, yeast extract 5g, triammonium hydrogen citrate 2g, glucose 20g, Tween 801mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.25g, agar 18g, distilled water 1000mL, pH 6.2-6.4)

[0026] experimental method

[0027] The dominant amine-producing enteric bacteria Enterococcus faecium in traditional Chinese sausages was coated on MRS agar medium; Lactobacillus plantarum xlbf001 bacterial liquid cultured at 37°C for 24 hours was centrifuged at 8000 rpm for 10 minutes, and a sterile pipette tip was used. Pipette the supernatant into a sterile centrifuge tube. Dip the sterilized filter paper with a diameter of 0.6 cm to take the supernatant, and place it on the above-mentioned MRS agar medium in...

Example Embodiment

[0029] Example 3

[0030] The role of Lactobacillus plantarum xlbf001 in the oxidation of biogenic amines.

[0031] Biogenic amines: tyramine, phenethylamine, tryptamine, and histamine.

[0032] Medium: Modified MRS liquid medium (10g peptone, 10g beef extract, 5g yeast extract, 2g triammonium hydrogen citrate, 20g glucose, 801mL Tween, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, sulfuric acid 0.25g manganese, 0.4g calcium carbonate, cysteine ​​hydrochloride, 7.5g milk powder, 65ml beer, 1000ml distilled water, pH 6.2~6.4)

[0033] experimental method

[0034] The experiment was divided into five groups. In each group, 500ug / ml of tyramine, phenethylamine, tryptamine, histamine and the control group were added to the modified MRS liquid medium, and sterilized at 115 °C for 15 minutes; after cooling, the experimental group was connected to plants Lactobacillus plantarum xlbf001, inoculum size of 10 6 cfu / ml, 37°C, shaker (85 r / min) for 72 h...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a lactobacillus plantarum strain of which the preservation number is CGMCC No.3166, which can be enriched in a modified MRS liquid culture medium. The lactobacillus plantarum strain is a white colony on an MRS solid culture medium, is straight, short-chained, Gram-positive and atrichous, has homofermentation, does not generate gas when fermenting glucose, generates acetic acid and lactic acid when fermenting ribose, and does not reduce nitrite. In application, the strain is activated and propagated and thalli are collected to form a fermenting agent which can inhibit the growth of amine producing bacteria in the fermenting production of meat products and generate biogenic amine oxidase which oxidizes and degrades tyramine, phenylethylamine, tryptamine and histamine. The invention has the advantages that the lactobacillus plantarum has strong growing ability, the fermenting agent has simple preparation technology and can effectively inhibit the growth and propagation of enterobacteria in the fermenting production of meat products and control the generation of biogenic amine, the generated biogenic amine oxidase can oxidize and degrade the contents of tyramine, phenylethylamine, tryptamine and histamine, and the lactobacillus plantarum strain as the fermenting agent for producing the fermented meat products can effectively reduce the accumulation of the biogenic amine.

Description

technical field [0001] The invention discloses an excellent lactic acid bacterium and its application, belonging to the field of biotechnology. Background technique [0002] Biogenic amines are a class of nitrogen-containing aliphatic, aromatic or heterocyclic organic compounds. There are mainly tyramine, histamine, putrescine, cadaverine, phenethylamine, tryptamine, spermine and spermidine. When it accumulates in a relatively high amount in the human body, it will produce toxicity, such as: the expansion of external blood vessels leads to high blood pressure and headache, and the contraction of intestinal smooth muscle causes abdominal cramps, diarrhea and vomiting, etc., and even life-threatening. [0003] Among the 8 common biogenic amines in food, spermine and spermidine are brought from the raw materials. Other biogenic amines are produced by the decarboxylation of amino acids catalyzed by amino acid decarboxylase produced by microorganisms, and mainly exist in ferment...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A23B4/12C12R1/25
Inventor 徐幸莲卢士玲周光宏刘登勇舒蕊华
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap