Biotechnology breeding method of regenerated functional rice

A biotechnology and functional rice technology, which is applied in the field of rice breeding, can solve the problems of increasing rice yield and the difficulty of rice planting area, and achieve the effect of increasing yield, not growing high, and having strong growth ability

Pending Publication Date: 2019-09-24
重庆汇鑫云强实业集团有限公司 +2
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present and for a long period of time in the future, it is impossible to increase rice production by expanding rice planting area, and it is also very difficult to maintain th

Method used

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Examples

Experimental program
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Effect test

Example Embodiment

[0031] Embodiment 1 of the present invention: A biotechnological breeding method for regenerating functional rice, including the following steps: S1: selecting barnyardgrass as the donor; S2: selecting Xiangwanxian No. 5 as the mother; S3: extracting the donor DNA S4: Introduce the donor's DNA and select Xiangwanxian No. 5; S5: Collect the distant hybrid seeds of step S4 Xiangwanxian No. 5; Step S6: Select the distant hybrid seeds collected in step S5 after seven generations Obtain seeds of a new line of stable growth.

[0032] The method of DNA extraction includes the following steps:

[0033] S31: Weigh 0.2g of the first to fourth tender leaves of the donor seedlings, remove the DNA contamination on the surface, and grind them into a fine powder with liquid nitrogen in a mortar;

[0034] S32: Transfer the donor fine powder into a 2ml centrifuge tube, immediately add 1000μl of CTAB extract preheated to 65°C, incubate at 65°C for 30min, and then mix at room temperature;

[0035] S33:...

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PUM

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Abstract

The invention discloses a biotechnology breeding method of regenerated functional rice. The method comprises the following steps of S1, selecting barnyard grass as a donor; S2, selecting Xiangwanxian No.5 as a parent body; S3, extracting DNA of the donor; S4, introducing guiding the DNA of the donor into the Xiangwanxian No.5; S5, collecting distant hybridization seeds of the Xiangwanxian No.5 in S4. By means of the biotechnology breeding method, the yield of the rice can be greatly increased, the DNA of the barnyard grass is introduced into the Xiangwanxian No.5, and the purpose that rice is harvested twice after being planted once can be achieved. The barnyard grass belongs to field weeds, has strong growth capability, and does not grow tall but grows dense. The first yield of the rice seeds cultivated by means of the method reaches 550-600 kilograms, and the yield of the regenerated rice reaches 300-400 kilograms.

Description

technical field [0001] The invention relates to a biotechnology breeding method for regenerative functional rice, belonging to the technical field of rice breeding methods. Background technique [0002] About 60% of my country's population takes rice as the staple food, so increasing rice production is a major issue for my country's sustainable agricultural development and ensuring food security. As the process of industrialization and urbanization is engulfing rice fields, to meet the challenges of population growth and ensure the steady development of the national economy, the fundamental way out is to increase the yield potential per unit area. Grain is a special strategic commodity, which is related to national economy and people's livelihood, political stability and social stability. Once there is a shortage of food and the food problem cannot be solved, economic development and social stability will lose their solid foundation. At present and for a long period of tim...

Claims

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Application Information

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IPC IPC(8): C12N15/87A01H5/10A01H6/46A01H1/02
CPCA01H1/02C12N15/87
Inventor 何登骥其他发明人请求不公开姓名
Owner 重庆汇鑫云强实业集团有限公司
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