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A kind of microbial electrode and preparation method thereof

A technology of microbial electrodes and electroactive microorganisms, applied in battery electrodes, biochemical fuel cells, circuits, etc., can solve the problems of unoptimized electricity generation performance, and achieve excellent electron transfer properties, high electroactivity, and improved electricity generation performance Effect

Active Publication Date: 2022-03-01
DATANG ENVIRONMENT IND GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current bacterial immobilization method only fixes the microorganisms on the electrode surface, and the microorganisms are "passively selected" on the electrode surface. It takes a long time for the competitive growth between microbial populations and the formation of active biofilms to reach equilibrium. Mid-range electricity performance has not been optimized

Method used

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  • A kind of microbial electrode and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Use carbon felt as the electrode base material, cut it into a 3cm diameter disc, soak it in acetone solution overnight, fully wash it with deionized water, and dry it; after drying, soak the carbon felt in a concentrated nitric acid solution with a concentration of 60% by mass In the nitric acid reflux device, boil for 0.5h, wash with deionized water and ethanol alternately; put the acidified carbon felt in 0.005g / mL1-ethyl-3-(3-dimethylaminopropyl) carbon di imine (EDC) and 0.005g / mL N-hydroxysuccinimide (NHS) solution, stirred at room temperature for 0.5h, wherein the soaking ratio is 20-30mL solution per gram of substrate; add a certain amount of 3-aminophenylboronic acid to make The concentration is 0.01g / mL, continue to react for 10h, wash thoroughly with ultrapure water, and then dry in vacuum at 20°C for 10h; soak the carbon felt with phenylboronic acid fixed on the surface for 1h in 2g / L glucose solution, and fully wash with ultrapure water washing. The modifie...

Embodiment 2

[0048] Use carbon brush as the electrode base material, soak in acetone solution overnight, fully wash with deionized water, and dry; after drying, soak the carbon brush in a concentrated nitric acid solution with a concentration of 70% by mass, and boil it in a nitric acid reflux device 6h, wash with deionized water and ethanol alternately; put the acidified carbon brush into 0.04g / mL LEDC and 0.02g / mLNHS solution, stir at room temperature for 2h, add a certain amount of 3-aminophenylboronic acid to make the concentration 0.2g / mL mL, after continuing to react for 14 hours, fully wash with ultrapure water, and then vacuum-dry at 30°C for 14 hours; soak the carbon brush with phenylboronic acid fixed on the surface in 4g / L glucose solution for 1 hour, and fully wash with ultrapure water; the modified carbon brush The brush substrate material was co-cultured with the Shewanellaoneidensis MR-1 suspension, rotated at 1000 rpm, and incubated for 7 days to obtain an electrode material...

Embodiment 3

[0050] The carbon nanotubes were soaked in acetone solution overnight, fully washed with deionized water, collected by centrifugation, and then dried; the dried carbon nanotubes were soaked in a concentrated nitric acid solution with a concentration of 65% by mass, and boiled in a nitric acid reflux device for 30 minutes After being collected by centrifugation, alternately wash with deionized water and ethanol, and centrifuge; put the acidified carbon nanotubes into 0.02g / mL LEDC and 0.01g / mLNHS solution, stir at room temperature for 1h, add a certain amount of 3-aminophenylboronic acid to make it Concentration is 0.1g / mL, continue to react for 12h, then collect by centrifugation, wash thoroughly with ultrapure water, and then dry in vacuum at 25°C for 12h; carbon nanotubes with phenylboronic acid immobilized on the surface are soaked in 8g / L glucose solution for 1h, and ultrapure Fully washed with water; the modified carbon nanotubes were co-cultured with G. sulfurreducens (AT...

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Abstract

The present invention relates to the field of microbial fuel cells, in particular to a microbial electrode and a preparation method thereof, wherein, a preparation method of a microbial electrode comprises the following steps: placing the acidified substrate in an aqueous solution containing a coupling agent, and adding phenylboronic acid substances to obtain an activated substrate; soaking the activated substrate in glucose solution, and then co-cultivating it with electroactive microorganisms to obtain a microbial electrode; the coupling agent is 1 A mixture of ‑ethyl‑3‑(3‑dimethylaminopropyl) carbodiimide and N‑hydroxysuccinimide; the phenylboronic acid is 3‑aminophenylboronic acid or 4‑dimethylaminobenzene Any one of boric acid; the electroactive microorganism is any one or both of Geobacter or Shewanella. Due to the electroactive biofilm covered on the surface of the microbial electrode prepared by the present invention, the surface has excellent electron transfer properties, thereby significantly improving the electricity generation performance of the microbial electrode.

Description

technical field [0001] The invention relates to the field of microbial fuel cells, in particular to a microbial electrode and a preparation method thereof. Background technique [0002] Microbial electrode is a combination of electrochemically active microorganisms or complete cells as catalysts or sensitive elements and electrodes. It decomposes the substrate through the metabolism of microorganisms and converts the chemical energy into electrical energy or electrical signals. s installation. Microbial electrodes can be used as microbial fuel cell anodes and electrochemical microbial sensor electrodes. [0003] In recent years, microbial fuel cells, as the most common microbial electrochemical system, have developed rapidly in the fields of power generation, hydrogen production, chemical synthesis, wastewater treatment, seawater desalination, etc. The key component for energy conversion is the microbial electrode as the anode. [0004] Microbial electrodes are the core co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01M4/88H01M4/90H01M8/16
CPCH01M8/16H01M4/9008H01M4/88Y02E60/50
Inventor 包文运吴晔姚琛周宾
Owner DATANG ENVIRONMENT IND GRP
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