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Microbial electrode and preparation method thereof

A technology of microbial electrodes and electroactive microorganisms, applied in battery electrodes, biochemical fuel cells, circuits, etc., can solve problems such as unoptimized electricity production performance, and achieve excellent electron transfer properties, high electroactivity, and improved electricity production performance Effect

Active Publication Date: 2021-05-25
DATANG ENVIRONMENT IND GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current bacterial immobilization method only fixes the microorganisms on the electrode surface, and the microorganisms are "passively selected" on the electrode surface. It takes a long time for the competitive growth between microbial populations and the formation of active biofilms to reach equilibrium. Mid-range electricity performance has not been optimized

Method used

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  • Microbial electrode and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Using carbon felt as the electrode base material, it was cut into a 3cm diameter disc, soaked in acetone solution overnight, fully washed with deionized water, and dried; after drying, the carbon felt was soaked in a concentrated nitric acid solution with a concentration of 60% by mass , boiled for 0.5h in a nitric acid reflux device, and washed alternately with deionized water and ethanol; put the acidified carbon felt into 0.005g / mL 1-ethyl-3-(3-dimethylaminopropyl)carbon two In imine (EDC) and 0.005g / mL N-hydroxysuccinimide (NHS) solution, stir at room temperature for 0.5h, the soaking ratio is 20-30mL solution per gram of substrate; add a certain amount of 3-aminophenylboronic acid to make The concentration was 0.01g / mL. After continuing the reaction for 10h, fully washed with ultrapure water and then vacuum dried at 20 °C for 10h; the carbon felt with phenylboronic acid immobilized on the surface was soaked in 2g / L glucose solution for 1h, and the ultrapure water wa...

Embodiment 2

[0048] The carbon brush was used as the electrode base material, soaked in acetone solution overnight, fully washed with deionized water, and dried; after drying, the carbon brush was soaked in a concentrated nitric acid solution with a concentration of 70% by mass, and boiled in a nitric acid reflux device 6h, alternately wash with deionized water and ethanol; put the acidified carbon brush into 0.04g / mLEDC and 0.02g / mL NHS solution, stir at room temperature for 2h, add a certain amount of 3-aminophenylboronic acid to make the concentration 0.2g / mL mL, continued the reaction for 14 h, washed thoroughly with ultrapure water, and dried in vacuum at 30 °C for 14 h; the carbon brush with phenylboronic acid immobilized on the surface was soaked in 4 g / L glucose solution for 1 h, and washed with ultrapure water; the modified carbon brush The brush base material was co-cultured with the Shewanella oneidensis MR-1 suspension, rotated at 1000 rpm, and after 7 days of incubation, the el...

Embodiment 3

[0050] Carbon nanotubes were soaked in acetone solution overnight, fully washed with deionized water, collected by centrifugation and dried; the dried carbon nanotubes were soaked in concentrated nitric acid solution with a concentration of 65% by mass, and boiled in a nitric acid reflux device for 30min , after centrifugal collection, alternately wash and centrifuge with deionized water and ethanol; put the acidified carbon nanotubes into 0.02g / mLEDC and 0.01g / mL NHS solutions, stir at room temperature for 1 h, and add a certain amount of 3-aminophenylboronic acid to make them The concentration was 0.1 g / mL, and the reaction was continued for 12 h, then centrifuged for collection, fully washed with ultrapure water, and then vacuum-dried at 25 °C for 12 h; the carbon nanotubes with phenylboronic acid immobilized on the surface were soaked in 8 g / L glucose solution for 1 h to obtain ultrapure Washed thoroughly with water; the modified carbon nanotubes were co-cultured with G.sul...

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Abstract

The invention relates to the field of microbial fuel cells, in particular to a microbial electrode and a preparation method thereof.The preparation method of the microbial electrode comprises the following steps that a base material obtained after acidification treatment is placed in an aqueous solution containing a coupling agent, phenylboronic acid substances are added, and an activated base material is obtained; and the activated base material is soaked in a glucose solution, and co-culturing the activated base material and electroactive microorganisms to obtain the microbial electrode; the coupling agent is a mixture of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, and the solvent is a mixture of N-hydroxysuccinimide and N-ethyl-3-(3-dimethylaminopropyl) carbodiimide; the phenylboronic acid substance is any one of 3-aminophenylboronic acid or 4-dimethylaminophenylboronic acid; the electroactive microorganisms are any one or two of geobacteria or shewanella. The surface of the prepared microbial electrode has excellent electron transfer property due to the electroactive biological membrane covered on the surface of the microbial electrode, so that the electricity generation performance of the microbial electrode is remarkably improved.

Description

technical field [0001] The invention relates to the field of microbial fuel cells, in particular to a microbial electrode and a preparation method thereof. Background technique [0002] A microbial electrode is a combination of electrochemically active microorganisms or intact cells as catalysts or sensitive elements combined with electrodes. It decomposes substrates through the metabolism of microorganisms and converts chemical energy into electrical energy or electrical signals. installation. Microbial electrodes can be used as microbial fuel cell anodes and electrochemical microbial sensor electrodes. [0003] In recent years, as the most common microbial electrochemical system, microbial fuel cells have developed rapidly in the fields of power generation, hydrogen production, chemical synthesis, wastewater treatment, seawater desalination, etc. The key component of energy conversion is the microbial electrode as the anode. [0004] Microbial electrodes are the core com...

Claims

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Application Information

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IPC IPC(8): H01M4/88H01M4/90H01M8/16
CPCH01M8/16H01M4/9008H01M4/88Y02E60/50
Inventor 包文运吴晔姚琛周宾
Owner DATANG ENVIRONMENT IND GRP
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