Two-phase anaerobic treatment device and process for establishing direct interspecies electron transfer based on ethanol-type fermentation

An anaerobic treatment device and electron transfer technology, which is applied in the field of environmental protection industry and can solve problems such as increasing the operating cost of anaerobic process

Inactive Publication Date: 2018-01-09
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, adding exogenous ethanol to enrich Geobacter will inevi

Method used

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  • Two-phase anaerobic treatment device and process for establishing direct interspecies electron transfer based on ethanol-type fermentation

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Effect test

Embodiment 1

[0044] A fully mixed anaerobic digester with an effective volume of 1L was used as the cylinder of the acidification phase, and 400mL of anaerobic sludge was inoculated with a sludge concentration of 8g / L. An upflow anaerobic sludge bed reactor with an effective volume of 2L was used as the cylinder of the methanogenic phase, and 1L of anaerobic sludge was inoculated with a sludge concentration of 20g / L. The organic waste water stored in the water inlet pool is simulated dairy waste water, and the water load is 53KgCOD / m 3 / d, the influent pH is 7.1. Mix concentrated HCI solution (industrial grade, concentration ≥ 95%) with water at a ratio of 1:3 to prepare a 4mol / L dilute HCI solution and store it in an acid adjustment pool. The hydraulic retention time of the acidification phase cylinder is controlled to be 6 hours. The pH in the cylinder is controlled at 4.3, and the temperature is controlled at 27°C. The hydraulic retention time of the methanogenic phase cylinder is co...

Embodiment 2

[0046] A fully mixed anaerobic digester with an effective volume of 1L was used as the cylinder of the acidification phase, and 400mL of anaerobic sludge was inoculated with a sludge concentration of 9g / L. An upflow anaerobic sludge bed reactor with an effective volume of 2L was used as the cylinder of the methanogenic phase, and 0.9L of anaerobic sludge was inoculated with a sludge concentration of 18g / L. The organic waste water stored in the water inlet pool uses actual landfill leachate, and the influent load is 79.2KgCOD / m 3 / d, the influent pH is 8.0. Mix concentrated HCI solution (industrial grade, concentration ≥ 95%) with water at a ratio of 1:3 to prepare a 4mol / L dilute HCI solution and store it in an acid adjustment pool. The hydraulic retention time of the acidification phase cylinder is controlled to be 4 hours. The pH in the cylinder is controlled at 4.5, and the temperature is controlled at 27°C. The hydraulic retention time of the methanogenic phase cylinder...

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Abstract

The invention discloses a two-phase anaerobic treatment device and process for establishing direct interspecies electron transfer based on ethanol-type fermentation. The process comprises the following steps: respectively taking a fully mixed anaerobic digester and an up-flow anaerobic sludge bed reactor as an acidification phase and a methanogenesis phase; respectively controlling the hydraulic retention time of the acidification phase and the methanogenesis phase, and the pH and temperature in the device; regulating the fermentation pH of the acidification phase to be 4.0 to 4.5 by using 2 to 4mol/L HCI solution, and running the process. The two-phase anaerobic treatment device and process can achieve the following effects of improving the content of ethyl alcohol in an acidized product;improving the abundance of geobacter in granule sludge of the methanogenesis phase and promoting the establishment of direct interspecies electron transfer with methanogens; accelerating the decomposition rate of organic acid and alcohol substances in the methanogenesis phase; mitigating the acidity accumulation and syntrophism metabolism stasis of the methanogenesis phase caused by high organicloading; and improving the methanogenesis efficiency. The technology has the advantages of low investment cost, simple operation, remarkable improvement effect, and can be applied to the upgrading andrebuilding of the existing two-phase anaerobic process for improving the resistance to high load wastewater impact.

Description

technical field [0001] The invention relates to the environmental protection industry, in particular to a treatment process for organic waste water. Background technique [0002] Anaerobic methanation technology is currently one of the most realistic and effective methods to realize the resource utilization and energy utilization of pollutants. It is generally believed that an efficient methanotrophic metabolic mechanism is the key to maintaining the stability of anaerobic methanation. In the past more than half a century, the main working mode of methanotrophic metabolism has been considered to be interspecific hydrogen transfer (IHT)—acidogenic bacteria decompose organic acids and alcohols into acetic acid and release hydrogen, which consumes hydrogen to produce methane Bacteria use hydrogen to reduce carbon dioxide to produce methane. However, the interspecies hydrogen chain between acidogenic bacteria and hydrogen-consuming methanogens is affected by hydrogen-producing...

Claims

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Application Information

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IPC IPC(8): C02F3/28
CPCY02E50/30
Inventor 张耀斌赵智强
Owner DALIAN UNIV OF TECH
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