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Heavy metal passivation and soil remediation type microbial agent and preparation method thereof

A microbial inoculum and soil remediation technology, applied in chemical instruments and methods, soil conditioning materials, fertilizer mixtures, etc., can solve the problems of limited heavy metal remediation ability and poor microbial stability, and achieve convenient operation, stable survival and reproduction, and combination. Reasonable effect

Active Publication Date: 2021-12-31
创想未来生物工程(北京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in the existing microbial remediation technology for heavy metals, such as poor stability of microorganisms. After being applied to the field, on the one hand, they have to compete with indigenous strains, and on the other hand, they have limited ability to repair heavy metals. Therefore, it is necessary to select suitable strains Combining to improve the competitiveness of strains in the soil and a good carrier to provide a good living environment for microorganisms is a research focus of the existing technology

Method used

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  • Heavy metal passivation and soil remediation type microbial agent and preparation method thereof
  • Heavy metal passivation and soil remediation type microbial agent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The heavy metal passivation and soil remediation type microbial bacterial agent of the present embodiment are prepared by the following methods:

[0025] (1) Preparation of carrier: mix diatomite, sepiolite, and dolomite in a mass ratio of 5:2:3 and then calcinate at 1200°C for 1 hour to obtain an inorganic carrier. Separately pulverize the inorganic carrier and the organic carrier. The organic carrier and the inorganic carrier passed through a 200-mesh sieve are fully mixed with a mass ratio of 2:3, sent to a granulator for granulation, and the particle size of the granulated product is controlled at 1 mm by conventional methods to obtain carrier particles;

[0026] (2) Microbial bacteria preparation: Bacillus subtilis, Bacillus megaterium, Bacillus peptone-like, Geobacter metalreducens, Saccharomyces, and Achromobacter were separately cultivated by conventional methods so that the number of effective viable bacteria was 1×10 9 The bacterial liquid of CFU / ml, obtains t...

Embodiment 2

[0031] The heavy metal passivation and soil remediation type microbial bacterial agent of the present embodiment are prepared by the following methods:

[0032] (1) Preparation of carrier: mix diatomaceous earth, sepiolite, and dolomite in a mass ratio of 5:2:3 and calcinate at 1000°C for 1.5 hours to obtain an inorganic carrier. Separately pulverize the inorganic carrier and the organic carrier, Fully mix the organic carrier and the inorganic carrier passed through a 200-mesh sieve with a mass ratio of 3:2, send them into a granulator for granulation, and use conventional methods to control the particle size of the granulated product at 2 mm to obtain carrier particles;

[0033] (2) Microbial bacteria preparation: Bacillus subtilis, Bacillus megaterium, Bacillus peptone-like, Geobacter metalreducens, Saccharomyces, and Achromobacter were separately cultivated by conventional methods so that the number of effective viable bacteria was 1×10 9 The bacterial liquid of CFU / ml, obt...

Embodiment 3

[0038] The heavy metal passivation and soil remediation type microbial bacterial agent of the present embodiment are prepared by the following methods:

[0039] (1) Preparation of carrier: mix diatomite, sepiolite, and dolomite in a mass ratio of 5:2:3 and then calcinate at 1200°C for 1 hour to obtain an inorganic carrier. Separately pulverize the inorganic carrier and the organic carrier. The organic carrier and the inorganic carrier passed through a 200-mesh sieve are fully mixed with a mass ratio of 1:1, sent to a granulator for granulation, and the particle size of the granulated product is controlled at 0.5 mm by conventional methods to obtain carrier particles;

[0040] (2) Microbial bacteria preparation: Bacillus subtilis, Bacillus megaterium, Bacillus peptone-like, Geobacter metalreducens, Saccharomyces, and Achromobacter were separately cultivated by conventional methods so that the number of effective viable bacteria was 1×10 9 The bacterial liquid of CFU / ml, obtains...

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Abstract

The invention relates to a heavy metal passivation and soil remediation type microbial agent and a preparation method thereof. The heavy metal passivation and soil remediation type microbial agent contains various microorganisms such as bacillus subtilis, bacillus megatherium, bacillus mucilaginosus, metal reduction geobacter and the like, and is loaded by a composite carrier prepared from an inorganic carrier and an organic carrier. After the heavy metal passivation and soil remediation type microbial agent is applied to soil, the microbial agent can stably survive and reproduce in the carrier, so that heavy metals in the soil can be effectively remedied, and the carrier contains silicon, calcium and magnesium elements, so that the pH value of the soil can be adjusted, and the heavy metals in the soil can be passivated.

Description

technical field [0001] The invention relates to a heavy metal passivation and soil restoration type microbial bacterial agent and a preparation method thereof. Background technique [0002] The treatment of heavy metal pollution in soil is a major problem in today's world, because heavy metal pollution in soil is an irreversible process, and heavy metals in soil are non-degradable and difficult to remove by physical and chemical methods. It is very difficult and expensive to remediate soil heavy metal pollution by traditional methods. In recent years, with the rapid development of mining, chemical industry, printing, dyeing and textile industry and the excessive application of chemicals in agricultural production, soil heavy metal pollution has become increasingly serious, posing safety hazards to human health. [0003] Due to the advantages of ecology, low cost, simplicity and efficiency, and wide application, microbial technology has gradually attracted people's attention...

Claims

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Application Information

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IPC IPC(8): C09K17/40C09K101/00C09K109/00
CPCC09K17/40C09K2109/00C09K2101/00
Inventor 陈敏余
Owner 创想未来生物工程(北京)有限公司
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