Method for extracting geobacter extracellular polymeric substances

A technology of extracellular polymers and geobacteria, applied in the field of microbiology, can solve the problems of changes in the characteristics of extracellular polymers and the lack of uniform standards for extraction methods

Inactive Publication Date: 2019-01-01
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many extraction methods for extracellular polymers, but there is no uniform standard for the extraction methods, and the characteristics of extracellular polymers will also change with the change of extraction methods

Method used

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  • Method for extracting geobacter extracellular polymeric substances
  • Method for extracting geobacter extracellular polymeric substances
  • Method for extracting geobacter extracellular polymeric substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Use the CHI1010C electrochemical workstation to cultivate the anode biofilm. The microbial fuel cell adopts a three-electrode system, in which the saturated calomel electrode is the reference electrode, and the 15mm*10mm*5mm cuboid graphite plate is the working electrode and the counter electrode. The electrochemical workstation runs Amperometric In i-t Curve mode, when the current runs to the highest point in the third cycle, the electrochemical workstation stops running. Disassemble the battery, remove the working electrode graphite plate, take the electrolyte to obtain soluble EPS (S-EPS), and rinse the graphite plate electrode with 0.9% NaCl solution to remove the soluble EPS (S-EPS) on the graphite plate. Scrape off the biofilm on the graphite plate, suspend it in 0.9% NaCl solution to get the biological suspension, take a certain volume of the suspension to measure the OD600 value, take a certain volume of the suspension and dry it, calculate its density, and ca...

Embodiment 2

[0035] 1) Use the CHI1010C electrochemical workstation to cultivate the anode biofilm. The microbial fuel cell adopts a three-electrode system, in which the saturated calomel electrode is the reference electrode, and the 15mm*10mm*5mm cuboid graphite plate is the working electrode and the counter electrode. The electrochemical workstation runs Amperometric In i-t Curve mode, when the current runs to the highest point in the third cycle, the electrochemical workstation stops running. Disassemble the battery, remove the working electrode graphite plate, take the electrolyte to obtain soluble EPS (S-EPS) S-EPS, and rinse the graphite plate electrode with 0.9% NaCl solution to remove the soluble EPS (S-EPS) on the graphite plate. ). Scrape off the biofilm on the graphite plate, suspend it in 0.9% NaCl solution to get the biological suspension, take a certain volume of the suspension to measure the OD600 value, take a certain volume of the suspension and dry it, calculate its densi...

Embodiment 3

[0039]1) Use the CHI1010C electrochemical workstation to cultivate the anode biofilm. The microbial fuel cell adopts a three-electrode system, in which the saturated calomel electrode is the reference electrode, and the 15mm*10mm*5mm cuboid graphite plate is the working electrode and the counter electrode. The electrochemical workstation runs Amperometric In i-t Curve mode, when the current runs to the highest point in the third cycle, the electrochemical workstation stops running. Disassemble the battery, remove the working electrode graphite plate, take the electrolyte to obtain soluble EPS (S-EPS) S-EPS, and rinse the graphite plate electrode with 0.9% NaCl solution to remove the soluble EPS (S-EPS) on the graphite plate. ). Scrape off the biofilm on the graphite plate, suspend it in 0.9% NaCl solution to get the biological suspension, take a certain volume of the suspension to measure the OD600 value, take a certain volume of the suspension and dry it, calculate its densit...

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Abstract

The invention discloses a method for extracting geobacter extracellular polymeric substances. A microbial fuel cell is utilized to culture a biological film, ultrasound and centrifugation are combinedto extract the geobacter extracellular polymeric substances in a layered mode, and soluble S-EPS, loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) can be extracted out. Bacteria damage in anextracting process is effectively reduced, and the extracting amount of the extracellular polymeric substances is improved. According to the method, the characteristics of the geobacter extracellularpolymeric substances are represented for the first time, and the extracted geobacter extracellular polymeric substances is favorable for environmental pollution rehabilitation; furthermore, the method has a certain application prospect in extracellular electron transfer pathways and environment rehabilitation.

Description

technical field [0001] The invention relates to a method for extracting extracellular polymers of Geobacter, belonging to the field of microorganisms. Background technique [0002] Microbial extracellular polymer EPS is mainly composed of polysaccharides, proteins and humic acids. It is a type of viscous organic polymer polymer secreted by microorganisms. It attaches to the surface of bacteria or surrounds them to form a protective layer, thus It enables microorganisms to survive under unfavorable conditions such as dryness, temperature and pH. Currently, extracellular polymers are divided into soluble extracellular polymers (S-EPS), loosely bound extracellular polymers (LB-EPS), and tightly bound extracellular polymers (TB-EPS). Distributed on the surface and surroundings of bacteria from the outside to the inside. There are many extraction methods for extracellular polymers, but there is no uniform standard for the extraction methods, and the characteristics of extracell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C08B37/00C08H7/00
CPCC07K1/30C07K1/34C07K1/36C08B37/0003C08H6/00
Inventor 林君庄莉杨贵芹麦绮君
Owner JINAN UNIVERSITY
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