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Microorganism continuously-fermenting corn stalk hydrolysate power generation method and battery

A technology for fermenting corn stalks and microbial fermentation, applied in biochemical fuel cells, fuel cells, circuits, etc., to achieve efficient resource utilization

Active Publication Date: 2018-01-23
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of agricultural waste, such as corn stalks, has not yet been published in relevant literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Hydrolysis of corn stalks

[0029] Utilize 1% H 2 SO 4 Mix with corn stalks at a mass ratio of 10% (w / w), and react at 121 °C for 30 minutes to obtain corn stalk hydrolyzate.

[0030] (2) Cultivation of strains

[0031] M. thermoacetica and Geobacter sulfurreducens were mixed with 10 g / L xylose, 1.0 g (NH 4 ) 2 SO 4 , 0.25 gMgSO 4 ·7H 2 O, 0.04 g Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O, 0.24 mg NiCl 2 ·6H 2 O, 0.29 mg ZnSO 4 ·7H 2 O, 0.017 mg Na 2 SeO 3 , 0.25 g cysteine·HCl·H 2 O, 5 g yeast extract, 7.5 gKH 2 PO 4 , 4.4 g K 2 HPO 4 , 0.415 g NaOH, 5 g NaHCO 3 culture medium at 100 rpm on a shaker at 60°C for 24 hours to prepare the bacteria liquid of Moorella thermoacetica and Geobacter sulfurreducens.

[0032] (3) Hydrolyzate of corn stalk fermented by Moorella thermoacetica

[0033] Adjust the pH value of the corn stalk hydrolyzate obtained in (1) to 6.8. Take 20 mL of the M. thermoacetica strain prepared in (2), and inoculate it into 200 mL of ...

Embodiment 2

[0038] A microbial fermentation corn stalk hydrolyzate biofuel cell, comprising a double-chamber container separated by a proton exchange membrane, an anode and a cathode arranged in two chambers of the double-chamber container, and the N in the double-chamber container 2 -CO 2The mixed gas, the bacteria solution of Geobacter sulfur reducing and the culture solution obtained by fermenting the hydrolyzate of corn stalks with Moorella thermoacetica. Wherein, the volume ratio of the culture solution and the bacteria solution of Geobacter sulfur reducing is 27:2. The anode is made of 40% waterproof carbon cloth impregnated with 1 M HCl, and the cathode is made of 0.5 mg / cm 2 Made of platinum 40% waterproof carbon cloth. The pH value of the corn stalk hydrolyzate is 6.8.

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Abstract

The invention relates to a microorganism continuously-fermenting corn stalk hydrolysate power generation method and a battery. The method comprises the following steps: hydrolyzing corn stalks to obtain corn stalk hydrolysate; fermenting the corn stalk hydrolysate in an anaerobic environment by utilizing a Moorella thermoacetica bacterial solution to obtain a culture solution; and injecting the culture solution and sulfur reduced geobacter bacterial solution into a double-chamber container isolated by a proton exchange membrane, reacting in an anaerobic environment, and outputting electricpower from an electrode of the double-chamber container. The battery comprises a double-chamber container isolated by a proton exchange membrane, an anode and a cathode which are arranged in two chambersof the double-chamber container, as well as N2-CO2 mixed gas, sulfur reduced geobacter bacterial solution and the culture solution obtained by fermenting the corn stalk hydrolysate with the Moorella thermoacetica in the double-chamber container. The agricultural waste corn stalks are converted into electric power by virtue of a bioengineering way, so that the high-efficiency recycling of the agricultural wastes is realized while the environment-friendly power generation is realized.

Description

technical field [0001] The invention relates to the technical field of new biological energy, in particular to a method for generating electricity by continuously fermenting corn stalk hydrolyzate by microorganisms, and a biofuel cell by fermenting corn stalk hydrolyzate by microorganisms. Background technique [0002] Microbial Fuel Cell (MFC) is a device that uses microorganisms to directly convert chemical energy in organic matter into electrical energy. Its basic working principle is: in the anaerobic environment of the anode chamber, the organic matter decomposes under the action of microorganisms and releases electrons and protons. The circuit is transmitted to the cathode to form a current, and the proton is transmitted to the cathode through the proton exchange membrane, and the oxidant (usually oxygen) obtains electrons at the cathode and is reduced and combined with the proton to form water. [0003] However, the microorganisms in the current microbial fuel cell t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M8/16H01M8/04186
CPCY02E60/50
Inventor 杨雪薇李思婷
Owner SHENZHEN UNIV
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