Microorganism continuously-fermenting corn stalk hydrolysate power generation method and battery
A technology for fermenting corn stalks and microbial fermentation, applied in biochemical fuel cells, fuel cells, circuits, etc., to achieve efficient resource utilization
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Embodiment 1
[0028] (1) Hydrolysis of corn stalks
[0029] Utilize 1% H 2 SO 4 Mix with corn stalks at a mass ratio of 10% (w / w), and react at 121 °C for 30 minutes to obtain corn stalk hydrolyzate.
[0030] (2) Cultivation of strains
[0031] M. thermoacetica and Geobacter sulfurreducens were mixed with 10 g / L xylose, 1.0 g (NH 4 ) 2 SO 4 , 0.25 gMgSO 4 ·7H 2 O, 0.04 g Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O, 0.24 mg NiCl 2 ·6H 2 O, 0.29 mg ZnSO 4 ·7H 2 O, 0.017 mg Na 2 SeO 3 , 0.25 g cysteine·HCl·H 2 O, 5 g yeast extract, 7.5 gKH 2 PO 4 , 4.4 g K 2 HPO 4 , 0.415 g NaOH, 5 g NaHCO 3 culture medium at 100 rpm on a shaker at 60°C for 24 hours to prepare the bacteria liquid of Moorella thermoacetica and Geobacter sulfurreducens.
[0032] (3) Hydrolyzate of corn stalk fermented by Moorella thermoacetica
[0033] Adjust the pH value of the corn stalk hydrolyzate obtained in (1) to 6.8. Take 20 mL of the M. thermoacetica strain prepared in (2), and inoculate it into 200 mL of ...
Embodiment 2
[0038] A microbial fermentation corn stalk hydrolyzate biofuel cell, comprising a double-chamber container separated by a proton exchange membrane, an anode and a cathode arranged in two chambers of the double-chamber container, and the N in the double-chamber container 2 -CO 2The mixed gas, the bacteria solution of Geobacter sulfur reducing and the culture solution obtained by fermenting the hydrolyzate of corn stalks with Moorella thermoacetica. Wherein, the volume ratio of the culture solution and the bacteria solution of Geobacter sulfur reducing is 27:2. The anode is made of 40% waterproof carbon cloth impregnated with 1 M HCl, and the cathode is made of 0.5 mg / cm 2 Made of platinum 40% waterproof carbon cloth. The pH value of the corn stalk hydrolyzate is 6.8.
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