Method for degrading protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens

Abstract

The invention discloses a method for degrading a protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens. The method comprises the following specific steps: S1, firstly inoculating the proteiniphilum acetatigenes in a culture solution, and then inoculating the geobacter sulfurreducens; S2, protein degradation effect: observing the degradation effect of protein inoculated with mixed bacteria for different days and different in inoculation proportions, and the result shows that the co-culture of the two bacteria is far better than pure culture of the proteiniphilum acetatigenes in terms of protein degradation effect; wherein the proteiniphilum acetatigenes and the geobacter sulfurreducens are inoculated in a proportion of 1: 4, the protein degradation rate of a co-culture system where the proteiniphilum acetatigenes grows for 2 days firstly and then the geobacter sulfurreducens is inoculated is the highest, the degraded proteiniphilum acetatigenes of the invention are protein degradation bacteria and can generate acetic acid, and the acetic acid can be used as a substrate of the sulfur-reducing geobacter and can be used as a substrate of the geobacter sulfurreducens. Compared with other protein degrading bacteria, the mixed system can degrade more proteins, and is simple and convenient to operate.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for co-culturing and degrading proteins by acetogenic proteophilic bacteria and sulfur-reducing Geobacter. Background technique [0002] Proteiniphilum acetatigenes TB107T is a Gram-negative, anaerobic protein-degrading bacterium, which was first isolated from the granular sludge of brewery wastewater, and can degrade yeast extract, peptone, pyruvic acid and other complex The substrate produces volatile fatty acids such as acetic acid, etc., and Geobactersulfurreducens PCA is also an anaerobic bacterium, which can use acetic acid, propionic acid, etc. as substrates. [0003] With the large-scale increase in the number of sewage treatment plants, a large amount of domestic sewage and a large amount of sludge are produced. In addition to a large amount of pollutants, the sewage and sludge also contain a large amount of protein. It is rich in a large amount of prote...

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Problems solved by technology

[0003] With the large-scale increase in the number of sewage treatment plants, a large amount of domestic sewage and a large amount of sludge are produced. In addition to a large amount of pollutants, the sewage and sludge also contain a large amount of protein. It is rich in a large amount of protein resources, amino acids, and rich in growth factors. Due to the difficulty of resource recovery, the random discharge of high-protein wastewater not only wastes resources but also causes certain damage to the ecological environment. Therefore, it is imminent to fully recycle and degrade such resources.

[0004] At present, the recovery and degradation methods of protein in sewage and sludge include protein extraction, degradation and differentiation treatment, and direct degradation of protein in sewage sludge, such as hot acid hydrolysis, hot alkali hydrolysis, and wet oxygen hydrolysis in chemical hydrolysis; physical methods Ultrasonic method; biological method such as enzymatic hydrolysis; combined methods such as ultrasonic combined with enzyme method, ultrasonic combined with acid method, etc., such as Chinese patent document CN109400671A adopts alkaline low-temperature hydrolysis method to treat sludge, breaks biological cell wall, releases protein, adopts large Pore ​​exchange resin refines the filtrate to obtain a higher degradable protein solution; however, these methods are complex, costly, unstable, and run for a long time. At the same time, just removing them will waste a lot of valuable nutrients

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