Method for degrading protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens

A technology of acetic acidophilic protein and sulfur reduction site, applied in the field of microorganisms, can solve the problems of complicated treatment, high cost, waste of nutrients, etc., and achieve the effect of simple and convenient operation, short treatment time, and promotion of degradation

Pending Publication Date: 2022-01-07
DONGGUAN UNIV OF TECH
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the large-scale increase in the number of sewage treatment plants, a large amount of domestic sewage and a large amount of sludge are produced. In addition to a large amount of pollutants, the sewage and sludge also contain a large amount of protein. It is rich in a large amount of protein resources, amino acids, and rich in growth factors. Due to the difficulty of resource recovery, the random discharge of high-protein wastewater not only wastes resources but also causes certain damage to the ecological environment. Therefore, it is imminent to fully recycle and degrade such resources.
[0004] At present, the recovery and degradation methods of protein in sewage and sludge include protein extraction, degradation and differentiation treatment,

Method used

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  • Method for degrading protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens
  • Method for degrading protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens
  • Method for degrading protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens

Examples

Experimental program
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Effect test

Embodiment A1

[0032] Take embodiment A1 as an example:

[0033] S1: Co-cultivation of two bacteria:

[0034] A: Preparation of culture medium: first add 500mL distilled water to a 1L Erlenmeyer flask, then add poly-peptone 2.5g / L, yeast extract 5.0g / L, tryptone 2.5g / L, glucose 10.0g / L, chloride Ammonium 1.5g / L, calcium chloride 0.024g / L, potassium chloride 0.1g / L, sodium dihydrogen phosphate 0.64g / L, dipotassium hydrogen phosphate 0.04g / L, vitamin solution 5mL, trace element solution 5mL, and then Add pure water to make the volume up to 1L, heat it in a water bath until it dissolves, add NaOH dropwise to adjust the pH to 6.9, then repack it into narrow-mouth bottles, insert the aeration needle into the narrow-mouth bottle, and inject 80% N 2 and 20%H 2 Mixed gas, aerated for 20 minutes, quickly capped the rubber stopper, placed the narrow-mouth bottle in an autoclave, sterilized at 121°C for 20 minutes, and then transferred to the ultra-clean bench for ultraviolet irradiation;

[0035] B...

Embodiment A1-A4

[0040] Embodiments A1-A4: According to the replacement of the inoculation time in the embodiment A1, the specific data are shown in Table 1, and the protein degradation rate was detected on the mixed bacterial solution, and the specific data are shown in Table 2.

[0041] Table 1 embodiment A1-A4 mixed bacterial liquid inoculation time

[0042] A1 A2 A3 A4 Inoculation time 0 1 2 3

Embodiment B1

[0047] Take embodiment B1 as an example:

[0048] S1: Co-cultivation of two bacteria:

[0049] A: Preparation of culture medium: first add 500mL distilled water to a 1L Erlenmeyer flask, then add poly-peptone 2.5g / L, yeast extract 5.0g / L, tryptone 2.5g / L, glucose 10.0g / L, chloride Ammonium 1.5g / L, calcium chloride 0.024g / L, potassium chloride 0.1g / L, sodium dihydrogen phosphate 0.64g / L, dipotassium hydrogen phosphate 0.04g / L, vitamin solution 5mL, trace element solution 5mL, and then Add pure water to make the volume up to 1L, heat it in a water bath until it dissolves, add NaOH dropwise to adjust the pH to 7.0, then repack it into a narrow-mouthed bottle, insert the aeration needle into the narrow-mouthed bottle, and inject 80% N 2 and 20%H 2 Mixed gas, aerated for 20 minutes, quickly capped the rubber stopper, placed the narrow-mouth bottle in an autoclave, sterilized at 121°C for 20 minutes, and then transferred to the ultra-clean bench for ultraviolet irradiation;

[00...

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Abstract

The invention discloses a method for degrading a protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens. The method comprises the following specific steps: S1, firstly inoculating the proteiniphilum acetatigenes in a culture solution, and then inoculating the geobacter sulfurreducens; S2, protein degradation effect: observing the degradation effect of protein inoculated with mixed bacteria for different days and different in inoculation proportions, and the result shows that the co-culture of the two bacteria is far better than pure culture of the proteiniphilum acetatigenes in terms of protein degradation effect; wherein the proteiniphilum acetatigenes and the geobacter sulfurreducens are inoculated in a proportion of 1: 4, the protein degradation rate of a co-culture system where the proteiniphilum acetatigenes grows for 2 days firstly and then the geobacter sulfurreducens is inoculated is the highest, the degraded proteiniphilum acetatigenes of the invention are protein degradation bacteria and can generate acetic acid, and the acetic acid can be used as a substrate of the sulfur-reducing geobacter and can be used as a substrate of the geobacter sulfurreducens. Compared with other protein degrading bacteria, the mixed system can degrade more proteins, and is simple and convenient to operate.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for co-culturing and degrading proteins by acetogenic proteophilic bacteria and sulfur-reducing Geobacter. Background technique [0002] Proteiniphilum acetatigenes TB107T is a Gram-negative, anaerobic protein-degrading bacterium, which was first isolated from the granular sludge of brewery wastewater, and can degrade yeast extract, peptone, pyruvic acid and other complex The substrate produces volatile fatty acids such as acetic acid, etc., and Geobactersulfurreducens PCA is also an anaerobic bacterium, which can use acetic acid, propionic acid, etc. as substrates. [0003] With the large-scale increase in the number of sewage treatment plants, a large amount of domestic sewage and a large amount of sludge are produced. In addition to a large amount of pollutants, the sewage and sludge also contain a large amount of protein. It is rich in a large amount of prote...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC12N1/20C02F3/34C02F2101/38
Inventor 刘倩陈加娇林辉吕斯濠杨立辉郭膘虎
Owner DONGGUAN UNIV OF TECH
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