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Sulfadimidine degrading bacterium S-07 and screening method thereof

A technology of sulfamethazine and S-07, applied in the field of sulfamethazine-degrading bacteria S-07 and its screening, can solve the problems of low antibiotic degradation efficiency and inability to apply high-temperature composting

Inactive Publication Date: 2016-08-17
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

This bacterium is a normal / low temperature bacterium, which cannot be used in high temperature composting, and the degradation efficiency of this bacterium to antibiotics is low

Method used

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  • Sulfadimidine degrading bacterium S-07 and screening method thereof
  • Sulfadimidine degrading bacterium S-07 and screening method thereof
  • Sulfadimidine degrading bacterium S-07 and screening method thereof

Examples

Experimental program
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Embodiment 1

[0023] Weigh 5.0 g of antibiotic pharmaceutical factory workshop sludge and place it in a 250 ml Erlenmeyer flask, add 100 ml of MMSM medium, in which the concentration of sulfamethazine is 5.0 mg / L, place it at 70°C, and the shaker speed is 100rpm Cultivate in the dark. Every 10 days of acclimatization, 5.0 ml of culture medium was taken to transfer once, and the concentration of antibiotics in the medium was increased by 5 mg / L each time until the concentration of sulfamethazine reached 50 mg / L. Take 0.1 ml of the culture solution and spread it on a selective plate with a sulfamethazine concentration of 50 mg / L, and culture it in a 70°C incubator. The colony growing on the plate was picked out, separated and purified to obtain degrading bacteria S-07.

[0024] Wherein, the preparation method of MMSM medium is: Na 2 EDTA·2H 2 O (0.018 g / L), FeSO 4 ·7H 2 O(0.013 g / L), CaCl 2 2H 2 O (0.013 g / L), MgSO 4 ·7H 2 O (0.25 g / L), Na 2 HPO 4 (7.5 g / L), KH 2 PO 4 (5 g / L), N...

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Abstract

The invention relates to a sulfadimidine degrading bacterium S-07 degraded by microorganisms and a screening method thereof. The sulfadimidine degrading bacterium S-07 is verified as Geobacillus, and the Genbank registration number of the bacterium 16S rDNA is KU588289. The sulfadimidine degrading bacterium is preserved in the China General Microbiological Culture Collection Center on April 21st, 2016, and the preservation number of the sulfadimidine degrading bacterium is CGMCC No. 12382. The sulfadimidine degrading bacterium S-07 well grows within the temperature range of 50-75 DEG C, has quite strong tolerance to sulfadimidine, and can efficiently degrade sulfadimidine in a liquid phase and a solid phase. The bacterium can be used for degrading and removing sulfadimidine residues in livestock excrement or sludge high-temperature compost, the removal rate is larger than 95%, and the sulfadimidine degrading bacterium S-07 has good industrial application prospects and environment benefits.

Description

technical field [0001] The invention relates to the technical field of environmental protection and microbial degradation of organic pollutants, in particular to a sulfamethazine-degrading bacterium S-07 and a screening method thereof. Background technique [0002] Sulfonamide antibiotics are one of the six types of antibiotics commonly used in livestock and poultry farming. They are widely used in the treatment of livestock and poultry animal diseases and added to feed to promote animal growth. The antibacterial mechanism of sulfonamide antibiotics is that they are structurally similar to p-aminobenzoic acid (PABA), which can compete with PABA to act on the dihydrofolate synthase in bacteria, thereby preventing PABA from being used as a raw material to synthesize the folic acid required by bacteria. Reduces the amount of metabolically active tetrahydrofolate, which is essential for bacteria to synthesize DNA, thus inhibiting bacterial growth and reproduction. Sulfamethazin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C12N1/02C05F3/00C05F7/00C05F17/00C12R1/01
CPCC05F3/00C05F7/00C05F17/00C12N1/02C12N1/36C12N1/205C12R2001/01C05F11/08Y02W30/40Y02A40/20
Inventor 汪印潘兰佳余广炜唐晓达李杰
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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