Immobilized bactericide capable of realizing in-situ restoration of polluted sediment and preparation method and application thereof

A technology for in-situ restoration and sedimentation, applied to the preparation of the above materials, immobilized bacterial agents for in-situ restoration of contaminated sediments, and the application of the above-mentioned materials in the field of in-situ restoration of contaminated sediments to speed up the reaction rate , Improve the removal effect

Active Publication Date: 2018-10-09
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to apply this mechanism in the actual water environment to promote the degradation of various pollutants is an important challenge for researchers in environmental microbiology and environmental engineering.

Method used

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  • Immobilized bactericide capable of realizing in-situ restoration of polluted sediment and preparation method and application thereof
  • Immobilized bactericide capable of realizing in-situ restoration of polluted sediment and preparation method and application thereof
  • Immobilized bactericide capable of realizing in-situ restoration of polluted sediment and preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0035] The preparation method of immobilized bacterial agent of the present invention, comprises the steps:

[0036] 1) Carry out pretreatment of Hangjin soil 2#: coarsely crush Hangjin soil 2# with a mortar, grind and directly pass through a 100-mesh sieve, and use 2.5mol / L H at a ratio of 1:10 (mass: volume) 2 SO 4 Acidify at 90°C for 2 hours, then add 5mol / L NaOH solution to adjust the pH value to 7.5, centrifuge the mud-water mixture, discard the supernatant, wash with pure water for 3-4 times, dry at 90°C, and put it in a horse After being fired at a high temperature of 750°C for 3 hours in a Furnace, it is ready for use, and finally a particle filler with a particle size of less than 0.15mm is obtained.

[0037] 2) Screening of bacterial strains: the microorganisms screened include Geobacter sulfurreducens strain DL1, ATCC52573), Geobacter metallireducens (ATCC53774), Shewanella oneidensis strain MR-1, ATCC700500.

[0038] 3) Purification and cultivation of strains: in...

Embodiment 1

[0046] 1) Preparation of Hangjin soil 2# loading matrix

[0047] The purchased Hangjin soil 2# is coarsely crushed with a mortar, ground and passed through a 100-mesh sieve, and 200g of the ground Hangjin soil 2# is weighed, and 2000ml of 2.5mol / L H 2 SO 4 , acidified in a water bath at 90°C for 2h, and then added 5mol / L NaOH solution to adjust the pH value to 7.5. Pour the mixed solution into a centrifuge tube, centrifuge at 8000rpm for 10min, pour off the supernatant, wash with pure water and centrifuge again, repeat 3-4 times. The precipitate was dried in an oven at 90°C for 2 hours, and then placed in a muffle furnace for 3 hours at a high temperature of 750°C to obtain a particle filler with a particle size of less than 0.15mm in mass of about 190g.

[0048] 2) Screening of strains

[0049] In this example, three kinds of bacteria capable of using iron oxides and humic substances as electron acceptors were screened out from more than 30 extracellular electricity-produc...

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Abstract

Immobilized bactericide capable of realizing in-situ restoration of polluted sediment is composed of Hangjin clay 2# loaded conductive microorganisms. A preparation method of the immobilized bactericide includes: 1), pretreating Hangjin clay 2# to obtain granular filler; 2), subjecting conductive microorganism strains to enlarged culture to obtain a to-be-inoculated bacteria solution, proportionally adding Hangjin clay 2# pretreated in the step 1, mixing in an anaerobic condition, standing, and removing supernatant to obtain the immobilized bactericide, wherein the conductive microorganism strains include geobacter sulfurreducens, geobacter metalreducens and shewanella spp.. The invention further discloses the preparation method of the immobilized bactericide and application of the immobilized bactericide in in-situ restoration of the polluted sediment.

Description

technical field [0001] The invention belongs to the field of water ecological restoration, and in particular relates to an immobilized bacterial agent for in-situ restoration of polluted sediments. [0002] The invention also relates to a method for the preparation of the aforementioned materials. [0003] The invention also relates to the use of the aforementioned materials for in situ remediation of contaminated deposits. Background technique [0004] In recent years, with the country's emphasis on ecological and environmental protection, especially the implementation of the lake chief system and river chief system, it has effectively promoted the development of water shoreline management, water pollution prevention and control, and water environment governance. For the lake ecosystem, after entering the water body, the nutrients input from the external source are partially degraded and absorbed, and most of them are enriched in the sediment, and migrate and transform in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C02F3/34C02F11/02C02F11/00C12R1/01
CPCC02F3/34C02F11/008C02F11/02C02F2103/007C12N1/20C12N11/14C02F3/341
Inventor 霍守亮张靖天席北斗马春子李小闯
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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