Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for in situ fluorescence labeling of Geobacter

A technology of fluorescent labeling and geobacteria, which is applied in the field of microorganisms, can solve the problems that bacillus cannot be in situ fluorescent labeling, cannot be applied, and has a short half-life.

Active Publication Date: 2021-06-04
FUJIAN AGRI & FORESTRY UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the synthesis of its chromophore requires oxygen molecules as an essential cofactor, so it cannot be applied in the anaerobic bacteria Geobacter
In addition, the half-life of GFP is short, which also limits its long-term in situ observation of biofilm formation.
Therefore, finding a fluorescent probe that can emit light under anaerobic conditions and has a long half-life and its labeling method is an effective strategy to solve the current in situ fluorescent labeling of Geobacter.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for in situ fluorescence labeling of Geobacter
  • A method for in situ fluorescence labeling of Geobacter
  • A method for in situ fluorescence labeling of Geobacter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Strains involved in this example: (1) Escherichia coli strain DH 5α chemically competent cells (purchased from Beijing Quanshijin Biotechnology Co., Ltd.) were used for gene cloning and plasmid amplification. (2) Geobactersulfurreducens PCA (purchased from the American Type Culture Collection, strain number ATCC-51573) was used to construct fluorescent protein-labeled strains.

[0038] The NBAF substratum formulation involved in cultivating bacterial strains in the present embodiment is as follows:

[0039]

[0040] *42g KH per liter of 100X NB salt solution 2 PO 4 , 22g K 2 HPO 4 , 20g NH 4 Cl, 38g KCl, 36g NaCl.

[0041] **Contains 2.14g NTA per liter of NB mineral solution; 0.1g MnCl 2 *4H 2 O; 0.3g FeSO 4 *7H 2 O; 0.17g CoCl 2 *6H 2 O; 0.2g ZnSO 4 *7H 2 O; 0.03g CuCl 2 *2H 2 O; 0.005g AlK(SO 4 ) 2 *12H 2 O; 0.005g H 3 BO 3 ;0.09g Na 2MnO 4 *2H 2 O; 0.11 g NiSO 4 *6H 2 O; 0.02g Na 2 WO 4 *2H 2 O.

[0042] ***Per liter of DL vitamin sol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for in situ fluorescence labeling of Geobacter. The PpFbFP fluorescent protein gene is transferred into Geobacter. The PpFbFP fluorescent protein marker of the present invention is not affected by oxygen, and can make the labeled Geobacter under anaerobic conditions. The strain fluoresces and has a long half-life, which enables long-term real-time observation of the movement trajectory, film formation process and group behavior of Geobacter.

Description

technical field [0001] The invention relates to a method for in-situ fluorescent labeling of Geobacter, belonging to the technical field of microorganisms. Background technique [0002] Geobacter is the electroactive bacteria with the largest number and widest distribution in the natural environment, and also the electroactive model strain with the most extensive research and the strongest electron transfer activity. It is widely distributed in freshwater sediments, groundwater sediments polluted by organic matter and heavy metals It has three functions of iron reduction, humus reduction and electricity generation. The electroactive biofilm formed by Geobacter has important application value in environmental remediation and bioengineering. It can produce electricity or other chemicals while treating pollutants, which helps pollution remediation technology to low input, low energy consumption, Transformation in the direction of low pollution. Using in situ fluorescent prote...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12Q1/04C12N15/74C12N15/66C12R1/01
CPCC07K14/43595C12N15/74C12Q1/04
Inventor 刘星靖宪月高鹏宇刘璐周顺桂
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com