Geobacterium engineering strain for passivating heavy metals and construction method of geobacterium engineering strain

A technology of engineering strains and construction methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of unstable effects of heavy metals and limited conditions in the repair process, and achieve the purpose of overcoming unstable effects and overcoming The effect of many restricted conditions and strong heavy metal tolerance in the repair process

Active Publication Date: 2022-04-29
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

For this reason, the present invention proposes a Geobacter engineering bacterial strain that inactivates heavy metals and its construction method. The Geobacter sulfurreducens HMP-1 constructed in the present invention can overcome the unstable and repairing effect of existing m...

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  • Geobacterium engineering strain for passivating heavy metals and construction method of geobacterium engineering strain
  • Geobacterium engineering strain for passivating heavy metals and construction method of geobacterium engineering strain
  • Geobacterium engineering strain for passivating heavy metals and construction method of geobacterium engineering strain

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Embodiment Construction

[0040] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0041] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.

[0042] A construction method of Geobacter engineering strains that passivate heavy metals

[0043] The specific construction steps are as follows:

[0044] (1) Construction of pUC-HMP:

[0045] Use the empty vector pUC19 (TaKaRa Company, Japan) as the carrier plasmid, and 5×In-Fusion HD EnzymePremix (TaKaRa Company, Japan), cysteine ​​gene, gentamicin resistance gene, PompJ strong promoter gene and target insertion sit...

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Abstract

The invention discloses a geobacter engineering strain for passivating heavy metals and a construction method of the geobacter engineering strain, the geobacter engineering strain for passivating the heavy metals is Geobacter sulfurreducens HMP-1, and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO.62189. The invention further discloses a construction method of the geobacter engineering strain for passivating the heavy metals. According to the heavy metal passivated geobacterium engineering strain, cysteine is marked on the pilus surface, and the cysteine in the pilus is combined with heavy metal ions to quickly form metal sulfide precipitates, so that the heavy metal removal rate reaches 70% or above under the condition of not depending on any substrate or highly enriched heavy metal plants, and the heavy metal removal rate can reach 70% or above under the condition of not depending on any substrate or highly enriched heavy metal plants. Therefore, the problem of Cd < 2 + > residue in the environment can be effectively improved, and the defects that the existing microbial remediation effect on heavy metals is unstable and the remediation process is limited by many conditions are overcome.

Description

technical field [0001] The invention belongs to the field of bioremediation of heavy metals, and in particular relates to a Geobacter engineering strain for inactivating heavy metals and a construction method thereof. Background technique [0002] According to the survey, heavy metal pollutants are currently the main pollutants in the global soil. The soil environment directly determines the yield and quality of agricultural products. Some heavy metal pollutants can even directly endanger human health through bioaccumulation. Therefore, soil metal pollution cannot be carried out. The management of things is taken lightly. In the context of today's increasing sustainable agricultural development, seeking efficient, stable and environmentally friendly heavy metal pollution remediation technology is also the focus and difficulty of ensuring the safety of agricultural products. [0003] At present, microbial passivation technology has become a priority for heavy metal pollution...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/74C12N15/65C12N15/11B09C1/10C12R1/01
CPCC12N15/74C12N15/65B09C1/105
Inventor 刘星靖宪月周顺桂
Owner FUJIAN AGRI & FORESTRY UNIV
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