Novel organic halide respiration geobacter and application thereof

An organic halogen and geological technology, applied in the field of microorganisms, can solve the problems of unreported and non-degradable organic chlorine of chlorinated alkanes

Active Publication Date: 2020-12-22
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only Desulfuromonas chloroethenica strain TT4b and Geobacter lovleyi strain SZ have been reported to use acetate as a carbon source and electron donor, but neither of them ca

Method used

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  • Novel organic halide respiration geobacter and application thereof
  • Novel organic halide respiration geobacter and application thereof
  • Novel organic halide respiration geobacter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Isolation, purification and identification of bacterial strains

[0028] (1) Configure the basic medium:

[0029] The composition of the anaerobic inorganic salt medium is: NaCl 1.0g / L, MgCl 2 ·6H 2 O 0.5g / L, KH 2 PO 4 0.2g / L, NH 4 Cl 0.3g / L, KCl 0.3g / L, CaCl 2 2H 2 O 0.015g / L, FeCl 2 4H 2 O 1.5mg / L, CoCl 2 ·6H 2 O190μg / L, MnCl 2 4H 2 O 100μg / L, ZnCl 2 70μg / L, H 3 BO 3 6μg / L, Na 2 MoO 4 2H 2 O 36μg / L, NiCl 2 ·6H 2 O 24μg / L, CuCl 2 2H 2 O 2μg / L, Na 2 SeO 3 ·5H 2 O 6 μg / L, Na 2 WO 4 2H 2 O 8μg / L, resazurin indicator 0.025% (w / v), L-cysteine ​​24mg / L, Na 2 S·9H 2 O48mg / L, dithiothreitol 77mg / L, NaHCO 32.52g / L (30mM), sodium acetate 0.41g / L (5mM), adjust the pH to 7.2-7.3. After autoclaving at 121°C, add multivitamins 15 minutes later, the final concentration of various vitamins in the medium is as follows: biotin 20μg / L, folic acid 20μg / L, pyridoxine hydrochloride 100μg / L, riboflavin 50μg / L, thiamine 50μg / L, pantothenic acid...

Embodiment 2

[0037] Example 2: Degradation performance identification of strain IAE to 1,2-DCA

[0038] Add 5mM sodium acetate to the anaerobic inorganic salt basal medium described in the above 100ml step (1). Sodium acetate is used as a carbon source and electron donor, and 10 μL (liquid phase concentration 1.23mM) 1,2-DCA is added through a micro-injector As an electron acceptor, 1% (v / v) was inoculated with pure IAE bacteria, and cultured statically at pH 7.2 and 30°C in the dark. 1,2-DCA and its degradation products were determined by gas chromatography (Agilent 7890B) in series with flame ionization detector (FID), loaded with Agilent DB-624 capillary column (60m×0.32mm×1.8μm). The gas chromatographic parameters are as follows: inlet temperature 200°C; heating program: 60°C, hold for 2min; then raise the temperature to 200°C at a rate of 25°C / min, hold for 1min; carrier gas is helium, column flow rate 3mL / min; The temperature of the FID detector is 300°C; the fuel gas is hydrogen, t...

Embodiment 3

[0040] Example 3: Degradation performance identification of strain IAE to 1,2-DCP

[0041] Add 5mM sodium acetate to the anaerobic inorganic salt basal medium described in the above 100ml step (1). Sodium acetate is used as a carbon source and electron donor, and 6 μL (liquid phase concentration: 0.57mM) 1,2-DCP is added through a micro-sampler As an electron acceptor, 1% (v / v) was inoculated with pure IAE bacteria, and cultured statically at pH 7.2 and 30°C in the dark. Quantitative monitoring of 1,2-DCP and its degradation product propylene by the above gas chromatographic conditions (see image 3 ).

[0042] The analysis results showed that the IAE strain could completely degrade 1,2-DCP (liquid phase concentration: 0.57mM) to propylene, and the period was about 144h.

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Abstract

The invention relates to the microbiological field, and discloses novel organic halide respiration geobacter and application of the novel organic halide respiration geobacter in bioremediation of chloralkane pollutants. A geobacter IAE bacterial strain is collected in the China General Microbiological Culture Collection Center (culture collection number CGMCC) on July 7, 2020, wherein the addressis No. 3, 1 Courtyard No., Beichenxi Road, Chaoyang District, Beijing City, Institute of Microbiology, Chinese Academy of Sciences, the zip code is 100101, and the collection number is 1.5298.

Description

technical field [0001] The invention relates to the field of microbes, and relates to a novel organohalogen breathing Geobacter and its application in bioremediation of chlorinated alkanes pollutants. Background technique [0002] Chlorinated alkanes are an important class of organic solvents and intermediates in the production of industrial chemicals, and are widely used in machinery manufacturing, electronic component cleaning, chemical engineering and other processes. Due to large-scale production and use, chlorinated alkanes have become common pollutants in industrial sites worldwide. For example, 1,2-dichloroethane (1,2-DCA) is a colorless and transparent oily liquid with high fat solubility, low flammability, high vapor pressure and low boiling point. In industry, it is often used as industrial raw materials, organic solvents and petroleum additives for the chemical synthesis of polyvinyl chloride and other chemicals; 1,2-dichloropropane (1,2-dichloropropane, referred...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00C02F3/34B09C1/10C12R1/01C02F101/36
CPCC12P3/00C02F3/34B09C1/10C02F2101/36C12R2001/01C12N1/205Y02A50/20
Inventor 严俊姜丽思杨毅李秀颖
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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