Media supplements and methods to culture human gastrointestinal anaerobic microorganisms

a technology of anaerobic microorganisms and media supplements, which is applied in the field of media supplements and methods to culture human gastrointestinal anaerobic microorganisms, can solve the problems of difficult culture of gut microorganisms, hampered studies of gut microorganisms, and novel culture techniques to grow these microorganisms. to achieve the effect of enhancing the growth of axenic bacteria in vitro
US20140342438A1Inactive Publication Date: 2014-11-20UNIVERSITY OF GUELPH
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
UNIVERSITY OF GUELPH
Publication Date
2014-11-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of effluent from a chemostat vessel in which a target bacterial ecosystem has been cultured. Methods of using the supplement for culturing or isolating anaerobic microbial strains or communities, particularly anaerobic bacteria from the human gut, are also provided.
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Description

RELATED APPLICATIONS

[0001] This application claims priority to U.S. provisional application No. 61 / 534,456, filed on Sep. 14, 2011, the entire contents of which are hereby incorporated by reference. This application is related to copending international PCT application titled “Method for Treatment of Disorders of the Gastrointestinal System,” filed Sep. 14, 2012, the entire contents of which are hereby incorporated by reference.FIELD OF THE INVENTION

[0002] This invention relates to methods for culturing microbial communities from the human distal colon and methods for isolating anaerobic microbes from such communities, as well as media supplements for use in such methods.BACKGROUND OF THE INVENTION

[0003] The human gut is the most densely inhabited ecosystem on Earth (Marchesi and Shanahan, 2007). Like other complex microbial ecosystems, the human microbiota has not been sampled to completion (Eckburg et al., 2005). This is because the individual species of the gut microbiota are diffic...

Claims

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