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Method for strengthening bagasse fermentation to produce hydrogen by using calcium carbonate and application

A technology of calcium carbonate and bagasse is applied in the field of microbial fermentation, which can solve the problems of low cost of raw materials, high hydrogen production, environmental pollution, etc., and achieves the effects of simple operation, increased hydrogen production, and increased fermentation hydrogen production.

Inactive Publication Date: 2015-06-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation process has low raw material cost, high hydrogen output, and combines hydrogen energy production with industrial waste utilization, which solves the environmental pollution problem of sugarcane sugar industry to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Utilize calcium carbonate to strengthen the method for hydrogen production of bagasse fermentation

[0035] (1) Preparation of Clostridium thermocellum (ATCC 27405) seed solution: Activate Clostridium thermocellum stored at -80°C in a 10mL vial at 55°C shaker (150rpm) for 72h, then 100mL The serum bottle was amplified and cultured for 72 hours under the condition of 55°C shaker (150rpm) to obtain OD 600 The seed liquid is about 1.3. Among them, MTC medium is used as the medium for activation and amplification. The main components of the medium are A', B, C, D, and E liquids. Syringe mixing, the solvent is deionized water. A' solution is 3g / L cellulose; B solution: potassium citrate 50g / L, citric acid monohydrate 31.25g / L, sodium sulfate 25g / L, potassium dihydrogen phosphate 25g / L, sodium bicarbonate 62.5g / L L; C solution: ammonium chloride 75g / L, urea 250g / L, yeast extract 50g / L; D solution: magnesium chloride hexahydrate 50g / L, ferrous chloride tetrahy...

Embodiment 2

[0038] Embodiment 2: Utilize calcium carbonate to strengthen the method for hydrogen production of bagasse fermentation

[0039] (1) The preparation of the thermophilic anaerobic Clostridium (ATCC 27405) seed solution is the same as in Example 1.

[0040] (2) Preparation of fermentation medium: the difference between fermentation medium and MTC medium is: the bagasse of 20g / L is used as the carbon source to replace the 3g / L cellulose in the MTC medium A' solution for the A liquid of the fermentation medium At the same time, 20mM calcium carbonate was added to liquid A, and the other ingredients and dosage ratios were the same as those of the MTC medium; the serum bottle containing the fermentation medium was sealed with a butyl rubber stopper and an aluminum cover, and repeated vacuuming and filling of 0.04MPa Nitrogen three times, and finally sterilized at 115°C for 20min.

[0041] (3) Hydrogen production by bagasse fermentation: inject the prepared seed solution into the st...

Embodiment 3

[0042] Embodiment 3: Utilize calcium carbonate to strengthen the method for hydrogen production of bagasse fermentation

[0043] (1) The preparation of the thermophilic anaerobic Clostridium (ATCC 27405) seed solution is the same as in Example 1.

[0044] (2) Preparation of fermentation medium: the difference between fermentation medium and MTC medium is: the bagasse of 20g / L is used as the carbon source to replace the 3g / L cellulose in the MTC medium A' solution for the A liquid of the fermentation medium At the same time, add 40mM calcium carbonate to liquid A, and other ingredients and dosage ratios are the same as MTC medium; seal the serum bottle containing the fermentation medium with a butyl rubber stopper and an aluminum cap, and repeat vacuuming and filling with a 0.04MPa bottle Nitrogen three times, and finally sterilized at 115°C for 20min.

[0045] (3) Hydrogen production by bagasse fermentation: inject the prepared seed solution into the sterilized fermentation m...

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PUM

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Abstract

The invention discloses a method for strengthening bagasse fermentation to produce hydrogen by using calcium carbonate and application. The method comprises the following steps: adding calcium carbonate in a basal fermentation culture medium to obtain a final fermentation culture medium; inoculating thermophilic anaerobic clostridium to perform anaerobic fermentation to obtain hydrogen. The bagasse in the method is common waste, and is in favor of directly degrading and utilizing the thermophilic anaerobic clostridium due to large specific surface area; the operation of adding a few of calcium carbonate on the basal fermentation culture medium is in favor of the utilization of the thermophilic anaerobic microorganism to the bagasse, and the hydrogen production capacity is greatly improved. Therefore, the method is especially suitable for preparing hydrogen.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method and application of using calcium carbonate to strengthen bagasse fermentation to produce hydrogen. Background technique [0002] With the shortage of fossil fuels and the environmental pollution problems caused by it becoming more and more serious, it is imminent to seek renewable and clean energy. At present, the research on biomass energy has been widely concerned by countries all over the world. As a representative of biomass energy, hydrogen is clean, efficient, and renewable, and is one of the ideal "green energy" in the future. At present, the raw materials for the production of biomass energy are still mainly food crops, so they are subject to great controversy. The use of lignocellulosic raw materials to produce biomass can conform to the principle of "not competing with people for food and not competing with food for land". my count...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/145
Inventor 朱明军田青青
Owner SOUTH CHINA UNIV OF TECH
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