A kind of genetically engineered bacteria, its construction method and its application in producing xylitol
A genetically engineered bacteria and genetic technology, applied in the field of genetic engineering and biology, can solve the problems of xylose reductase catalytic efficiency, cell growth rate reduction, etc., to achieve the effects of increased production rate, recovery of growth defects, and reduced utilization rate
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Embodiment 2
[0185] Embodiment 2 utilizes the example of engineering bacterium HK402 to produce xylitol
[0186] 1. Engineering bacteria use corncob hemicellulose hydrolyzate to produce xylitol
[0187] In order to verify whether the constructed engineering bacteria can utilize hemicellulose hydrolysis to produce xylitol, the engineering bacteria HK402 was used to carry out shake flask fermentation (250ml, liquid volume is 50ml).
[0188] (1) Inoculate the overnight seed medium with engineering bacteria HK402 at 2%, and cultivate it at 30°C for 8 hours to obtain the seed liquid;
[0189] The formula of seed medium and fermentation medium is: 1L of medium contains 4-6g Na 2 HPO 4 ,2~5gKH 2 PO 4 ,1~2g NH 4 Cl, 1~5g NaCl, 1~5mM MgSO 4 ,1~5mM CaCl 2 , 2-10g / L yeast extract.
[0190] (2) Concentrate the hemicellulose hydrolyzate with a rotary evaporator until the xylose content is 20%-50%, sterilize the concentrated hemicellulose hydrolyzate and add it to the sterilized fermentation med...
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