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Method for strengthening caproic acid fermentation by exogenously adding inorganic carbon source

An inorganic carbon source, calcium carbonate technology, applied in the field of waste recycling, can solve the problems of high salt, high cost, accumulation and so on

Active Publication Date: 2020-06-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The form and concentration of inorganic carbon source maintenance / supplementation affect the fermentation characteristics of mixed flora to a certain extent: It has been reported that Na 2 CO 3 、K 2 CO 3 Such as fully soluble inorganic carbon sources can quickly increase the concentration of inorganic carbon and bring pH buffering capacity, but in the long-term fermentation process, it may cause monovalent cations (such as Na + , K + ) accumulation, so that the fermentation process faces the problem of high salt, and the cost is relatively high

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  • Method for strengthening caproic acid fermentation by exogenously adding inorganic carbon source
  • Method for strengthening caproic acid fermentation by exogenously adding inorganic carbon source
  • Method for strengthening caproic acid fermentation by exogenously adding inorganic carbon source

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Effect test

Embodiment 1

[0048] Embodiment 1: the production of caproic acid fermented liquid

[0049] The raw materials used in the experiment are the naturally acidified fermentation broth and the inoculated sludge fermentation mixture obtained in the patent application number 201911354619.1, wherein:

[0050] Natural acidification group: first add water to the fruit and vegetable waste to obtain a fermentation system with a total solid concentration (TS) of 8% and a volatile solid (VS) content of 78g-VS / L, and then place the fermentation system at a temperature of 34 to Ferment for 17 days under the conditions of 36° C., pH 4.0-4.5, and rotation speed 60 rpm to obtain a fermentation liquid (this fermentation liquid is ethanol-type acidified fermentation liquid).

[0051] Inoculation sludge group: first add water to the fruit and vegetable waste to obtain a fermentation system with a total solid concentration (TS) of 8% and a volatile solid (VS) content of 78g-VS / L, and then use 70g-VS / L Inoculum a...

Embodiment 2

[0053] Embodiment 2: the selection of inorganic carbon source

[0054] Add inorganic carbon source in the fermented mixed solution that finally obtains that embodiment 1 obtains, can be divided into CO 2 Absorption group, blank control group, Na 2 CO 3 (200mg / L) dosing group and CaCO 3 (10g / L) group (the carbon concentration of the two groups is basically the same). The CO in which the experiment was set up 2 The realization method of the adsorption group is: the top of the anaerobic caproic acid fermentation device is connected with the device for carrying saturated sodium hydroxide solution, while the reaction devices of the remaining experimental groups are connected with 3mol / L phosphoric acid solution (pH is about 3, which is lower than that of saturated carbonic acid solution). pKa, ensure no CO 2 Dissolved in water), regularly sample and detect the liquid phase products, aiming at the number of homoacetogenic bacteria and hydrogen-eating methanogenic bacteria in di...

Embodiment 3

[0063] Embodiment 3: the selection of calcium carbonate dosage

[0064] Adopt the method of embodiment 2, set different calcium carbonate dosage gradients as experimental variable, set and add 5,15,20,25g / L CaCO respectively 3 Dosing amount of calcium carbonate as different concentrations. Samples were taken regularly for detection, and biophase samples were taken for the number of homoacetogenic bacteria and hydrogen-eating methanogens in different periods of time by fluorescence quantitative PCR and high-throughput sequencing.

[0065] The initial pH was adjusted by adding 3mol / L NaOH from an external source, and it was controlled at 7.5±0.1, and the temperature was controlled at 30°C. The headspace was evacuated with nitrogen for 5 minutes before the reaction was started to ensure an anaerobic environment in the reaction apparatus. Liquid sampling is performed by anaerobic extraction with a needle syringe, and the obtained liquid is sealed and stored at 4°C for a short pe...

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Abstract

The invention discloses a method for strengthening caproic acid fermentation by exogenously adding an inorganic carbon source, and belongs to the field of waste recycling. The method comprises the following steps: adding calcium carbonate into a fermentation mixed solution as an inorganic carbon source; wherein the adding amount of the calcium carbonate is 5-25 g / L relative to the mass volume concentration of the fermentation mixed solution. According to the method, calcium carbonate powder is externally added into a fermentation system at the initial stage of fermentation, so that an inorganic carbon source in the fermentation system can be continuously maintained, the relative abundance of a main microorganism clostridium kluyveri for producing caproic acid bacteria is improved, and theproduction of caproic acid is enhanced.

Description

technical field [0001] The invention relates to a method for strengthening hexanoic acid fermentation by adding inorganic carbon sources from an external source, and belongs to the field of waste recycling. Background technique [0002] The caproic acid conversion of waste is a biotechnology with great application potential, in which the proliferation of Clostridium kluyveri (caproic acid bacteria) increases its dominance in the mixed flora, which can provide a basis for obtaining higher-intensity caproic acid production and strengthen caproic acid production. The number of acid bacteria can guarantee the production of caproic acid. Caproic acid bacteria through acetyl-CoA and CO 2 combined to generate pyruvate to form caproic acid bacteria cells, which means that CO 2 Therefore, it becomes an essential carbon source for caproic acid bacteria Clostridium kluyveri. [0003] However, since the anaerobic caproic acid phase fermentation process cannot provide a continuous sup...

Claims

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Application Information

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IPC IPC(8): C12P7/40C12N1/20C12R1/145
CPCC12N1/20C12P7/40
Inventor 黄振兴余江南廖家林阮文权
Owner JIANGNAN UNIV
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