Method for directionally producing succinic acid by CO2 biotransformation

A technology for biotransformation and production of succinic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as difficulty in succinic acid, low solubility, and poor directional transformation effect

Active Publication Date: 2021-04-09
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But CO 2 The solubility in water is not high (1.13g / L, 37°C), the gas-liquid mass transfer rate is slow, and a large amount of by-products (lactic acid, formic acid and acetic acid) are easily accumulated during the biological fermentation process, resulting in low yield of succinic acid. CO 2 The directional conversion effect is poor, which makes it difficult to realize the production of succinic acid by biological fermentation to meet the requirements of industrial production

Method used

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  • Method for directionally producing succinic acid by CO2 biotransformation
  • Method for directionally producing succinic acid by CO2 biotransformation
  • Method for directionally producing succinic acid by CO2 biotransformation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] a CO 2 The method for the directional production of succinic acid through biotransformation, the concrete steps are as follows:

[0113] 1) Pipette 5 mL of the pure strain A. succinogenes 130Z solution and insert it into 100 mL of growth medium sterilized by high pressure steam at 121°C for 20 minutes for cultivation. The formula of the growth medium is: 0.75g potassium dihydrogen phosphate, 0.75g dipotassium hydrogen phosphate, 0.45g 2-ethanesulfonic acid, 5g yeast extract, 2.5g corn steep liquor, 0.006g sodium selenite, 1.008 g sodium chloride, 0.008g sodium tungstate, 0.5g sodium hydroxide, 1.5g ferrous chloride tetrahydrate, 0.19g cobalt chloride hexahydrate, 0.1g manganese chloride tetrahydrate, 0.07g zinc chloride, 0.006g Boric acid, 0.036g sodium molybdate, 0.024g nickel chloride hexahydrate, 0.002g copper chloride dihydrate, 0.5g magnesium chloride hexahydrate, 0.3g ammonium chloride, 0.3g potassium chloride, 0.015g calcium chloride dihydrate, 10mg Resazurin (...

Embodiment 2

[0133] a CO 2 The method for the directional production of succinic acid through biotransformation, the concrete steps are as follows:

[0134] 1) Pipette 5 mL of the pure strain A. succinogenes 130Z solution and insert it into 100 mL of growth medium sterilized by high pressure steam at 121°C for 20 minutes for cultivation. The formula of the growth medium is: 0.75g potassium dihydrogen phosphate, 0.75g dipotassium hydrogen phosphate, 0.45g 2-ethanesulfonic acid, 5g yeast extract, 2.5g corn steep liquor, 0.006g sodium selenite, 1.008 g sodium chloride, 0.008g sodium tungstate, 0.5g sodium hydroxide, 1.5g ferrous chloride tetrahydrate, 0.19g cobalt chloride hexahydrate, 0.1g manganese chloride tetrahydrate, 0.07g zinc chloride, 0.006g Boric acid, 0.036g sodium molybdate, 0.024g nickel chloride hexahydrate, 0.002g copper chloride dihydrate, 0.5g magnesium chloride hexahydrate, 0.3g ammonium chloride, 0.3g potassium chloride, 0.015g calcium chloride dihydrate, 10mg Resazurin (...

Embodiment 3

[0155] Repeat Example 2, the difference is that in step 6), the acid neutralizer in the mixed biological fermentation system is calcium carbonate.

[0156] The fermentation system of this embodiment will CO 2 The analysis results of liquid substances biotransformed into succinic acid are shown in Table 3 below:

[0157] Table 3: Analysis results of liquid substances in the reactor

[0158] time h Glucosemg / L Succinic acidmg / L Lactic acidmg / L Formic acidmg / L Acetic acidmg / L 0 59982.09 0.00 0.00 0.00 0.00 4 52855.52 1521.68 234.65 358.15 481.81 6 482541.34 13478.27 271.73 1216.12 1487.28 12 343885.07 22902.49 865.19 2651.67 5290.05 18 264550.41 23453.86 4598.52 3705.1 6040.21 24 188753.7 35024.81 4821.61 4236.74 6028.78 36 9983.71 38323.20 5692.77 5200.2 6720.54 48 10547.9 39776.54 7254.56 6313.41 7631.26 60 6547.8 40578.43 8902.56 7954.8 8457.9 72 0.00 42861.49 9595.93 8...

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Abstract

The invention discloses a method for directionally producing succinic acid by CO2 biotransformation. The method comprises the following steps of: preparing a culture medium suitable for the growth of pure strains for producing succinic acid, inoculating the pure strains for producing succinic acid for culturing, and obtaining pure bacteria for producing succinic acid in an exponential growth period; treating a precursor of an active metal catalyst, and grinding to obtain solid catalyst particles; adding a substrate, an acid neutralizer and the solid catalyst particles into a biological fermentation culture medium to obtain a mixed biological fermentation culture medium; inoculating the pure bacteria for producing succinic acid into the mixed biological fermentation culture medium, and uniformly mixing under an anaerobic condition to obtain a biological fermentation system for producing succinic acid by CO2 transformation; and introducing sufficient CO2 into a reactor, and producing succinic acid under an anaerobic condition. According to the method disclosed by the invention, the substrate, the acid neutralizer and the catalyst are coupled with pure bacteria fermentation to form an optimized biological fermentation system, and the optimized system can accelerate the fixation and utilization of CO2 and can stably and directionally produce high-concentration succinic acid.

Description

technical field [0001] The present invention relates to a kind of method of succinic acid production. More specifically, a CO 2 A method for the directed production of succinic acid through biotransformation. Background technique [0002] At present, the growing population and expanding industrialization have led to a sharp increase in global energy consumption, and at the same time lead to a large amount of CO 2 emissions, causing serious problems such as global temperature rise and glacier melting. Therefore, the search for renewable energy sources and reduction of CO 2 Issues such as emissions need to be resolved urgently. Compared with conventional physical and chemical technologies, carbon capture and storage through anaerobic microbial fermentation technology is an eco-friendly means, which can effectively reduce CO in the atmosphere. 2 while being able to CO 2 Utilize resources and convert them into high value-added chemicals or fuels. [0003] Succinic acid (a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12N1/38C12N1/20B01J23/89B01J27/22B01J27/25B01J21/04C12R1/01
CPCC12P7/46C12N1/38C12N1/20B01J23/8906B01J27/22B01J27/25B01J21/04
Inventor 王雯赵晴张燚杨紫怡
Owner BEIJING UNIV OF CHEM TECH
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