A detoxification method for lignocellulosic raw material prehydrolyzed sugar solution

A lignocellulosic raw material, pre-hydrolysis technology, applied in the direction of biofuel, fermentation, etc., can solve the problems of fermentation sugar loss, secondary pollution, poor effect, etc., to achieve the effect of improving sugar utilization rate, ethanol production and fermentation performance

Active Publication Date: 2018-10-19
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter what kind of pretreatment method is used, inhibitors will inevitably be produced, and the inhibitors are mainly weak acids (formic acid, acetic acid and levulinic acid), furan substances (furfural, 5-hydroxymethylfurfural) and phenolic substances, etc. , these inhibitors have seriously affected the subsequent yeast ethanol fermentation. In order to improve the efficiency of subsequent fermentation, effective removal of fermentation inhibitors, that is, "detoxification" is a necessary step to improve fermentation performance
[0003] There are many detoxification methods for lignocellulosic raw materials, mainly including steam stripping, activated carbon adsorption, calcium hydroxide overneutralization, electrodialysis, ion exchange, laccase treatment, etc. However, these detoxification methods generally have the effect of a single detoxification method Poor, secondary pollution during the detoxification process, and serious loss of fermentable sugar, etc.

Method used

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  • A detoxification method for lignocellulosic raw material prehydrolyzed sugar solution
  • A detoxification method for lignocellulosic raw material prehydrolyzed sugar solution
  • A detoxification method for lignocellulosic raw material prehydrolyzed sugar solution

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Experimental program
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Effect test

Embodiment 1

[0027] The steps for obtaining the concentrated liquid of lignocellulosic raw material prehydrolyzed sugar solution are as follows:

[0028] (1) Dilute acid steam explosion pretreatment: corn stalks were collected in Hohhot, Inner Mongolia. The mass fractions of cellulose and hemicellulose in corn stover were 37.17% and 22.84%, respectively. Corn stalks are soaked in sulfuric acid with a mass fraction of 0.50% to 1.00% for 1 to 3 hours, dehydrated to a solid content of 30% to 40%, treated at 1.6 to 2.0MPa for 5 to 10 minutes, and instantly open the discharge valve of the blasting device. Spray the material into the trough.

[0029] (2) Water washing pretreatment of corn stalks: wash the material three times with warm water with a solid-to-liquid ratio of 1:15, collect the filtrate (that is, the prehydrolyzed sugar solution of acidic blasted corn stalks) and filter residues for later use.

[0030] (3) Concentration of pre-hydrolyzed sugar solution: the corn stalk pre-hydrolyz...

Embodiment 2

[0037] The detoxification treatment of the lignocellulosic raw material prehydrolyzed sugar solution concentrate prepared in Example 1, the process is as follows:

[0038] 1) Washing of ionic liquid: Pipette 5 mL of 1-hexyl-3-methylimidazolium tetrafluoroborate [C 6 mim][BF 4 ], 1-octyl-3-methylimidazolium tetrafluoroborate [C 8 mim][BF 4 ] The ionic liquid and distilled water were mixed at a ratio of 1:1 and placed in a shaker, and reacted at 30°C and 150r / min for 20min. After washing with water, the mixed phase of water and ionic liquid was centrifuged at 4000r / min for 10min, and after the centrifugation, it was allowed to stand for 20min to separate the layers, and the ionic liquid was collected, and the water-saturated ionic liquid was used as the extraction agent.

[0039] 2) Detoxification method: pipette 5mL of 1-butyl-3-methylimidazolium hexafluorophosphate C 4 mim[PF 6 ], 1-hexyl-3-methylimidazolium hexafluorophosphate C 6 mim[PF 6 ], 1-octyl-3-methylimidazoliu...

Embodiment 3

[0046] [C 8 mim][BF 4 ] Determination of extraction equilibrium time

[0047] 1) [C of ionic liquid 8 mim][BF 4 ]Washing: pipette 5mL of 1-octyl-3-methylimidazolium tetrafluoroborate [C 8 mim][BF 4 ] The ionic liquid and distilled water were mixed at a ratio of 1:1 and placed in a shaker, and reacted at 30°C and 150r / min for 20min. After washing with water, the mixed phase of water and ionic liquid was centrifuged at 4000r / min for 10min, and after the centrifugation, it was allowed to stand for 20min to separate the layers, and the ionic liquid was collected, and the water-saturated ionic liquid was used as the extraction agent.

[0048] 2) Detoxification method: pipette 5mL of 1-octyl-3-methylimidazolium tetrafluoroborate [C 8 mim][BF 4 ] In five 50mL triangles, add 5mL pre-hydrolyzed sugar liquid concentrate (pH adjusted to 2) according to the ratio of 1:1, mix, place in a shaker, and react under the conditions of 30°C and 150r / min for 10, 20, 40, 60, 80 minutes. Af...

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Abstract

The invention discloses a detoxification method for a wood fiber raw material pre-hydrolysis sugar liquid. According to the detoxification method, a pre-hydrolysis concentrated sugar liquid obtained after dilute sulphuric acid steam explosion pretreatment is taken as a raw material and an ionic liquid is taken as an extraction agent for removing of fermentation inhibitors in the wood fiber raw material pre-hydrolysis concentrated sugar liquid, wherein the ionic liquid is a disubstituted imidazole cationic liquid. The detoxification method has the advantages that the organic ionic liquid is adopted for extraction treatment on a wood fiber raw material pre-hydrolysis concentrated sugar liquid, and the treatment result shows that on the premise that a little fermented sugar is lost, the removing rates of formic acid, acetic acid, levulinic acid, furfuraldehyde, hydroxymethyl furfural and total phenolics in the prehydrolysis concentrated sugar liquid reach 64.54%, 54.88%, 100%, 100%, 87.08% and 70.44% respectively; in the same period, the sugar utilization rate is improved by 74.34%, the ethanol yield is improved by 82.91%, and the fermentation circle is shortened.

Description

technical field [0001] The invention belongs to the technical field of biodegradation or biotransformation of lignocellulosic raw materials to produce fuel ethanol, chemical platform compounds and other bio-based products, and specifically relates to a novel green detoxification method for prehydrolyzing sugar liquid of lignocellulosic raw materials. Background technique [0002] As a large agricultural country, China has abundant renewable biological resources, and the development of lignocellulosic raw materials to produce fuel ethanol is considered to be one of the most promising biomass energy sources. Cellulosic biorefinery of fuel ethanol must go through three key technical links: raw material pretreatment, cellulase hydrolysis and monosaccharide fermentation. Lignocellulosic raw materials are mainly composed of cellulose, hemicellulose and lignin, a dense supramolecular complex, which has low accessibility to cellulase, and lignocellulosic raw materials must be pretre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 朱均均杨金龙荣亚运高露陈欣张宇康勇强徐勇欧阳嘉赖晨欢李鑫余世袁
Owner NANJING FORESTRY UNIV
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