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Method for producing medium-chain alcohol through fermentation of exogenous medium enhanced anaerobic microorganisms

An anaerobic microorganism and medium strengthening technology is applied in the field of exogenous medium strengthening anaerobic microorganism fermentation production of medium-chain alcohol, which can solve the problems of restricting industrialization development, low gas-liquid mass transfer efficiency, and low yield.

Active Publication Date: 2020-11-10
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Saccharomyces cerevisiae can synthesize a variety of medium-chain alcohols by itself; Kennes and others have successfully used a variety of pure bacteria to produce medium-chain alcohols such as butanol and hexanol, but the yields are relatively low, and it is far from reaching industrial mass production so as to replace gasoline as fuel. Purpose
In addition, compared with chemical processes, the problems of slow reaction rate and low gas-liquid mass transfer efficiency in syngas fermentation also limit its industrial development.

Method used

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  • Method for producing medium-chain alcohol through fermentation of exogenous medium enhanced anaerobic microorganisms
  • Method for producing medium-chain alcohol through fermentation of exogenous medium enhanced anaerobic microorganisms
  • Method for producing medium-chain alcohol through fermentation of exogenous medium enhanced anaerobic microorganisms

Examples

Experimental program
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Effect test

Embodiment 1

[0137] A kind of domestication method of anaerobic medium chain alcohol microorganism, concrete steps are as follows:

[0138] 1) Configure the medium, add 100mM acetic acid in the medium;

[0139] 2) Add culture medium and inoculate anaerobic microorganisms;

[0140] 3) Regulating temperature and pH, feeding synthesis gas, and reacting in a shaker reactor;

[0141] 4) The headspace CO pressure is adjusted to 0.3atm and maintained for 15-60 days;

[0142] 5) When the 24-hour headspace CO consumption in the reactor is >50%, adjust the headspace CO pressure to 0.7atm for 10-30 days;

[0143] 6) When the 24-hour headspace CO consumption in the reactor is >50%, adjust the headspace CO pressure to 1 atmCO for 10-30 days;

[0144] 7) When the 24-hour headspace CO consumption in the reactor is >50%, the acclimatization is completed, and anaerobic medium-chain alcohol-producing microorganisms are obtained.

[0145] In this embodiment, the temperature is controlled at 35° C., the i...

Embodiment 2

[0147] A method for producing medium-chain alcohols in a single-phase reactor by mixing an exogenous medium with anaerobic alcohol-producing microorganisms, the specific steps are as follows:

[0148] 1) Weigh chloroplatinic acid, dissolve it in ethylene glycol, and stir to make it dissolve evenly;

[0149] 2) Weigh Fe 2 o 3 powder, drop the solution obtained in step 1) into Fe 2 o 3 in, stir to mix thoroughly;

[0150] 3) Transfer the material prepared in step 2) to a drying oven, and dry it in an air atmosphere at 150°C for 12 hours;

[0151] 4) After drying the catalyst in step 3) at 400°C, use H 2 The / CO volume ratio is 1:1 and the synthesis gas is reduced for 12 hours to obtain an inorganic solid catalyst (external medium iron-supported platinum catalyst);

[0152] 5) Put the activated sludge sterilized by high-pressure steam at 120°C for 60 minutes into the anaerobic fermentation reactor, and add the necessary medium; keep the temperature at 35°C, and cultivate an...

Embodiment 3

[0167] A method for producing medium-chain alcohols in a two-phase reactor by mixing an exogenous medium with anaerobic alcohol-producing microorganisms, the specific steps are as follows:

[0168] 1) Weigh chloroplatinic acid, dissolve it in ethylene glycol, and stir to make it dissolve evenly;

[0169] 2) Weigh Fe 2 o 3 powder, drop the solution obtained in step 1) into Fe 2 o 3 in, stir to mix thoroughly;

[0170] 3) Transfer the material prepared in step 2) to a drying oven, and dry it in an air atmosphere at 150°C for 12 hours;

[0171] 4) After drying the catalyst in step 3) at 400°C, use H 2 The / CO volume ratio is 1:1 and the synthesis gas is reduced for 12 hours to obtain an inorganic solid catalyst (external medium iron-supported platinum catalyst);

[0172] 5) Put the activated sludge sterilized by high-pressure steam at 120°C for 60 minutes into the anaerobic fermentation reactor, and add the necessary medium; keep the temperature at 35°C, and cultivate and a...

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Abstract

The invention discloses a method for producing medium-chain alcohol by exogenous medium enhanced microorganisms. The method comprises the following steps of pretreating an exogenous medium, and performing grinding to obtain exogenous medium particles; pretreating anaerobic microorganisms and performing inoculating into a culture medium; inflating synthesis gas into the culture medium, and culturing and domesticating the microorganisms; inoculating anaerobic medium-chain alcohol production microorganisms, and performing mixing with the exogenous medium particles to obtain a fermentation systemfor producing medium-chain alcohol; and inflating synthesis gas into the culture medium containing a fermentation system, and performing reacting under anaerobic conditions to produce the medium-chainalcohol. The method also comprises two schemes of producing the medium-chain alcohol in a single-phase reactor and a two-phase reactor; According to the method, the fermentation system formed by anaerobic medium-chain alcohol production microorganisms and an exogenous medium can accelerate the absorption and conversion process of the microorganisms on the synthesis gas, and the medium-chain alcohol is produced. The exogenous medium in the fermentation system is not liable to inactivate in the reaction system, anaerobic microorganism death is not liable to cause, and the system can stably operate for 2-36 months.

Description

technical field [0001] The invention relates to a production method of medium-chain alcohols. More specifically, it relates to a method for the production of medium-chain alcohols by strengthening anaerobic microbial fermentation with an exogenous medium. [0002] technical background [0003] With the rapid development of modern industry, people's demand for fuel is constantly increasing. However, the reserves of fossil fuels are constantly decreasing, which leads to an urgent need for new and more sustainable energy sources. Large-scale development and utilization of renewable resources as clean energy is becoming increasingly important. Biomass is a renewable and clean resource. Through biotechnology, biomass can be converted into a renewable alternative energy source. [0004] Syngas is CO 2 , CO and H 2 As the main component, it is used as a raw material gas for chemical raw materials. Syngas can be produced by gasification of solid fuels such as coal or coke, it c...

Claims

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Application Information

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IPC IPC(8): C12P7/04C12P7/16C12N1/36
CPCC12P7/04C12P7/16C12N1/36Y02E50/10
Inventor 王雯马铭锐张燚刘昊鹏刘广青杨紫怡苏本生
Owner BEIJING UNIV OF CHEM TECH
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