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Chinese medicinal herba epimedii and propolis flavone immunopotentiator

The technology of an immune enhancer and ketone is applied in the field of immune adjuvant of livestock and poultry to achieve the effects of improved efficacy, controllable quality and advanced dosage form.

Active Publication Date: 2010-10-27
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] Technical Problems The present invention aims at the urgent need for efficient and safe immune enhancers or vaccine adjuvants in the current animal disease prevention and control technology, and provides a new type of traditional Chinese medicine immune enhancer made from Epimedium extract and propolis flavonoids

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Embodiment Construction

[0015] 1. Preparation process

[0016] (1) Preparation of Epimedium extract Taking the yield of Epimedium extract and Epimedium polysaccharide as an index, the amount of water added, boiling time and extraction times were carried out in an orthogonal test to determine the optimum yield of Epimedium extract. The extraction process is to add 20 times of water and decoct twice, the first time is 1.5h, and the second time is 1h. Weigh 15g of Epimedium decoction pieces, decoct in water according to the above process, combine the filtrates, concentrate under reduced pressure at 85°C to 835ml, and obtain the Epimedium extract solution.

[0017] (2) Preparation of propolis flavonoids On the basis of repeated comparisons, the extraction process of propolis flavonoids was determined. Weigh 5 g of wine-made propolis, add 75 ml of 95% ethanol to dissolve it, let stand, filter, recover ethanol from the filtrate to 65 ml, and obtain a propolis flavonoid solution.

[0018] (3) Preparation ...

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Abstract

The invention relates to a Chinese medicinal herb epimedii and propolis flavone immunopotentiator, which is called the herb epimedii and propolis flavone immunopotentiator for short and belongs to the field of immunologic adjuvant for livestock and poultry. Each 1,000 ml of liquid medicine is prepared from 15 grams of herb epimedii and 5 grams of propolis flavone by the following steps of: adding water, decocting the herb epimedii twice, merging percolate and concentrating the percolate to prepare 870 ml of herb epimedii extract; dissolving propolis, which is processed with rice wine, with ethanol, filtering and concentrating the dissolved propolis to prepare 130 ml of propolis; and mixing the 130 ml of propolis and the herb epimedii extract to prepare the product. Immunity tests prove that the herb epimedii and propolis flavone immunopotentiator can remarkably stimulate the multiplication of lymphocytes of chicken in vitro, remarkably improve the antibody tilter of serum when used together with Newcastle vaccine for chicken, promote the multiplication of the lymphocytes, enhance the cellular immunity and humoral immunity of the chicken and improve the immune response of vaccines.

Description

1. Technical field [0001] The invention relates to a traditional Chinese medicine Huofengone immune enhancer, which is made of Epimedium extract and propolis flavonoids, referred to as Huofengone immune enhancer for short, and belongs to the field of livestock and poultry immune adjuvants. 2. Background technology [0002] Livestock and poultry infectious diseases have always been the primary problem that endangers the development of animal husbandry, especially the major viral infectious diseases that are currently prevalent. So far, there is no effective treatment method, and vaccine prevention is still the most important measure. Due to various reasons, most vaccines can only exert a good anti-epidemic effect if they are used in conjunction with immune enhancers or immune adjuvants. However, chemical immune adjuvants such as oil emulsions and aluminum gels widely used at home and abroad often have disadvantages such as large side effects, strong local irritation, carcinog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/296A61K39/39A61P37/04A61K35/64A61K35/644
Inventor 胡元亮刘家国范云鹏王德云张宝康
Owner NANJING AGRICULTURAL UNIVERSITY
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