Live vaccine (CC gene stock) of cytokine adjuvant in animal immunopotentiator for treating chicken new castle disease

A chicken Newcastle disease and cytokine technology, applied in the direction of antiviral agents, virus antigen components, antibody medical components, etc., can solve the problems of low immune function of animal populations, reduced animal production performance, immune system disorders, etc., and achieve good clinical application effect, Guaranteeing human health and enhancing humoral immunity

Inactive Publication Date: 2006-04-19
金扩世 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to feed quality, improper use of antibiotics and chemical drugs, environmental factors, and invasion of pathogenic microorganisms, the aquaculture industry in ...

Method used

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Examples

Experimental program
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Embodiment Construction

[0011] The specific plan is as follows:

[0012] 1. Calf thymus - bleaching - extraction of connective tissue and fat - mincing - homogenization - repeated freezing and thawing - drying - extraction - centrifugation - ultrafiltration - thymosin ultrafiltrate - intermediate product inspection.

[0013] 2. Pig spleen—rinsing—removal of connective tissue and fat—mashing—homogenization—repeated freezing and thawing—centrifugation—ultrafiltration—transfer factor ultrafiltrate—intermediate product inspection.

[0014] 3. Chicken Newcastle disease attenuated strain (CC strain) - chicken embryo fibroblasts - nutrient solution for maintenance solution - adjust PH value - observe cell lesions - freeze-thaw - attenuated cell culture - intermediate product inspection.

[0015] 4. Prepare 10mg / ml thymosin ultrafiltrate—prepare 5mg / ml transfer factor ultrafiltrate—prepare every 0.1ml containing 10 8.5 TCID 50 Attenuated cell cultures - mixed in a ratio of 0.5:1:0.1.

[0016] 5. Freeze-dr...

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Abstract

An animal immunopotentiator, cytokine adjuvant-chicken Newcastle disease vaccine (cc strain) is prepared by coordination of the cytokine (thymopeptide or transfer factor) with low-virulent immunoactivator of Newcastle disease.

Description

technical field [0001] The invention belongs to the field of biological products, in particular to an animal immune enhancer cytokine adjuvant chicken Newcastle disease live vaccine (CC strain). Background technique [0002] 1. In August 1996, a virus strain was isolated from a chicken farm in Changchun. After chicken embryo and cell culture, electron microscope observation, gene cloning and sequence analysis, hemagglutination spectrum determination, red blood cell agglutination value determination, virus content determination, specificity Experiments, determination of the minimum immune dose, determination of the average death time of chicken embryos with the minimum lethal dose, determination of the pathogenicity index in the brain, and toxicity tests on one-day-old chickens have proved that it is a naturally attenuated strain of Newcastle disease. The vaccine preparation process research, vaccine safety experiment, immunization dose and immunization route screening test, ...

Claims

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Application Information

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IPC IPC(8): A61K39/17A61K39/39A61P31/14
Inventor 金扩世金宁一
Owner 金扩世
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