Preparation method of chitosan newcastle disease vaccine nanoparticles

A technology of Newcastle disease and chitosan, which is applied in the field of preparation of chitosan Newcastle disease vaccine nanoparticles, achieves the effects of high safety performance, reduced dosage, and prolonged action time

Inactive Publication Date: 2012-01-25
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method of chitosan newcastle disease vaccine nanoparticles
  • Preparation method of chitosan newcastle disease vaccine nanoparticles
  • Preparation method of chitosan newcastle disease vaccine nanoparticles

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specific Embodiment approach 1

[0013] Specific embodiment one: the preparation method of chitosan Newcastle disease vaccine nanoparticle of present embodiment, carry out according to the following steps: one, chitosan is dispersed in sterilized distilled water, under magnetic stirring, adding volume concentration is 36% Acetic acid, continue to stir 5~15min, filter with 0.22 μm filter, leave standstill until air bubble disappears, promptly obtain chitosan solution; 10 8.5 EID 50 Newcastle disease L-series virus liquid, after stirring and mixing evenly, then dropwise add 2-3mL of cross-linking agent sodium tripolyphosphate (TPP) with a concentration of 1.0mg / mL, and then at room temperature and under sterile conditions at 1000-1400r / min Magnetic stirring for 10-15min to obtain solution A; 3. Centrifuge solution A at 4°C, 10000-15000r / min for 30-40min, take the precipitate, wash the precipitate with sterile water 3-5 times, and then vacuum freeze-dry for 22 ~25h, promptly obtain chitosan Newcastle disease ...

specific Embodiment approach 2

[0045] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the volume ratio of the quality of chitosan and distilled water in step one is 1g: 917mL. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0046] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the mass of chitosan in step one and the volume ratio of acetic acid are 1g: 83mL. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a preparation method of chitosan newcastle disease vaccine nanoparticles, and relates to the preparation method of the chitosan newcastle disease vaccine nanoparticles. The method is that: 1, the chitosan is dispersed in the sterilized distilled water, under the magnetic stirring, the acetic acid is added to be stirred, filtered and carried out standing until the bubbles are eliminated, i.e. the chitosan solution is obtained; 2, the chitosan solution is taken and is dripped with the newcastle disease L-series virus liquid to be stirred and mixed uniformly, then the cross-linking agent is dripped, then the solution A is obtained after being stirred under normal temperature and aseptic conditions; and 3, the solution A undergoes centrifugal treatment, the sediment is taken out, is washed by sterile water, and then is vacuum freezed and dried, i.e. the chitosan newcastle disease vaccine nanoparticles are obtained. The vaccine nanoparticles has strong stability and long medicine release time, under the premise of maintaining the drug effect, the dosage is reduced, the toxic reaction side effect is reduced or avoided, and the immune protection period can be prolonged.

Description

technical field [0001] The invention relates to a preparation method of chitosan Newcastle disease vaccine nanoparticles. Background technique [0002] Newcastle disease (ND) is an acute, highly contagious, and fatal infectious disease of chickens and turkeys caused by Newcastle disease virus (NDV). It often presents with sepsis and is harmful to the poultry industry. One of the most important infectious diseases, it is classified as a Class I infectious disease by the International Veterinary Bureau. The most fundamental measure to control ND is effective vaccination. At present, the commonly used vaccines are attenuated live vaccines and inactivated vaccines, but both of them have certain limitations in practical application. ND oil emulsion inactivated vaccine can produce a higher level of blood circulating antibodies after injection of immunized chickens, but it has limitations in the mucosal system against respiratory and digestive tract pathogenic infections, and it i...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K39/17A61K47/36A61P31/14
Inventor 赵凯
Owner HEILONGJIANG UNIV
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