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Virus strain capable of being used for preparing VII type newcastle disease vaccine and encoding gene thereof

A technology of Newcastle disease virus and coding gene, which is applied in the field of preparation of virus strain and its coding gene of type VII Newcastle disease vaccine, which can solve the problems of weak persistence of epidemic prevention effect, weak infectivity and low immune response

Active Publication Date: 2016-08-24
GUONIAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Therefore, all walks of life are looking forward to and earnestly looking forward to developing a virus strain of type VII Newcastle disease vaccine that can solve the defects of traditional technologies such as low immune response, weak infectivity, and weak persistence of epidemic prevention effects.

Method used

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  • Virus strain capable of being used for preparing VII type newcastle disease vaccine and encoding gene thereof
  • Virus strain capable of being used for preparing VII type newcastle disease vaccine and encoding gene thereof
  • Virus strain capable of being used for preparing VII type newcastle disease vaccine and encoding gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5

[0062] "Virus Strains Collected from Field Cases"

[0063] First, according to figure 1 The collection steps of the virus strain of the Newcastle disease vaccine of type VII were collected from the poultry farms once infected with Newcastle disease (NVD), and histopathological samples were collected from the lungs, trachea, intestines, and brains of infected chickens. Collect 5 samples, each weighing 10 grams. At room temperature, the five histopathological samples were respectively placed in MEM medium (modified minimal essential medium) containing 2% fetal bovine serum, and fully ground with a tissue grinder (homogenizer); the suspensions were taken respectively, Then centrifuge at 1,500 rpm for 15 minutes, and then filter with a 0.2 μm filter membrane; take the supernatant liquid as samples V1-V5 to be tested. "Chicken Embryo Allantoic Cultivation Virus Strain"

[0064] Next, take 50ml of the samples V1-V5 to be tested and inoculate them into the allantoic cavity of 9-da...

Embodiment 6～10

[0074] Identification of Newcastle Disease Virus

[0075] 1. Mean death time (MDT)

[0076] At room temperature, from 10 dilutions in PBS 6 times, 10 7 times, 10 8 times, 10 9 In the diluted solution containing the virus strain samples CB-ND01~CB-ND06 of embodiment 1~embodiment 5 times, each get 0.1ml, inoculate in five 9~10 day old SPF chicken embryos respectively, then place in 37 ℃ Cultivate in a special incubator, perform egg inspection at the frequency of 2 times a day, record the death time of chicken embryos, and carry out a total of 7 consecutive days, and calculate the minimum lethal dose (the minimum lethal dose, MDT).

[0077] Table 3 shows the MDT identification results of the NDVs of Examples 6 to 10.

[0078] table 3

[0079]

[0080]

[0081] According to the MDT test result of the CB-ND02, CB-ND04, CB-ND06 Newcastle disease virus strain of embodiment 6 to embodiment 10 of table 3, the MDT that can confirm is all between 44 hours to 48 hours, thus CB...

Embodiment 11～15

[0089] The ICPI identification results of the NDVs of Examples 6 to 10 are listed in Table 4.

[0090] Table 4

[0091]

Example 11

Example 12

Example 13

Example 14

Example 15

virus strain sample

CB-ND01

CB-ND02

CB-ND03

CB-ND04

CB-ND06

Infect the host

SPF chicken

SPF chicken

SPF chicken

SPF chicken

SPF chicken

ICPI

1.79

0.1

1.75

0.3

1.74

virus identification

Vw

vs.

Vw

vs.

vs.

[0092] According to the ICPI test results of the CB-ND02, CB-ND04, and CB-ND06 Newcastle disease virus strains of Examples 11 to 15 in Table 4, it can be confirmed that the average values ​​of ICPI are all between 1.7 and 1.8, so CB-ND02 , CB-ND04, and CB-ND06 are strong virus strains, and it can be confirmed that the virus strain of the present invention is significantly better than the virus strains of current commercially available vaccines, for example, BC virus str...

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Abstract

The invention provides a virus strain capable of being used for preparing a VII type newcastle disease vaccine and an encoding gene thereof. The virus strain is Genotype VII Newcastle disease virus (VII NDV) / CB-ND02 with the preservation number of CCTCC NO:V201548, VII NDV / CB-ND04 with the preservation number of CCTCC NO:V201555, or VII NDV / CB-ND06 with the preservation number of CCTCC NO:V201556. The virus strain can effectively improve immunity of a host to newcastle disease viruses and produce antibody immune sustaining force in a poultry body, is applicable to research, development and preparation of a newcastle disease vaccine and also can be used for manufacturing an inactivated or attenuated type vaccine.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to the field of immunology, in particular to a virus strain and its encoding gene which can be used to prepare type VII Newcastle disease vaccine. Background technique [0002] The research report pointed out: Newcastle disease (ND) is the most common malignant infectious disease among various types of poultry diseases. Since Newcastle disease was discovered in 1926, it has spread to most countries in the world. Regional prevalence varies. [0003] Chicken Newcastle disease was first discovered in Indonesia in 1926, and soon it was discovered in Newtown, England. After that, it was recorded in all countries in the world. Newcastle disease (ND) is an acute infectious disease caused by Newcastle disease virus (NDV). It first broke out in Newcastle, Indonesia and the United Kingdom in 1926, hence the name Newcastle disease virus (Alexander DJ, 1982). It belongs to the family Paramyxovir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/17A61P31/14
CPCA61K39/12C12N7/00C12N2760/18121C12N2760/18134
Inventor 林建宏
Owner GUONIAN IND CO LTD
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