Chicken subunit four-combination vaccine and preparation and application thereof

A vaccine and chicken embryo technology, applied in the field of avian vaccine manufacturing, can solve the problems of short immunization period, easy to cause respiratory side effects, and interference with laboratory diagnosis of virus isolation, etc.

Active Publication Date: 2013-04-03
QINGDAO VLAND BIOTECH INC +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although the Newcastle disease live vaccine is effective, cheap and easy to use, it has a short immunization period and is easily interfered by maternal antibodies, and it is easy to cause respiratory side effects and interfere with laboratory diagnosis of virus isolation. Therefore, since 1980, Newcastle disease inactivated vaccines have been used Vaccine has gradually become one of the routine methods for preventing and controlling Newcastle disease in chicken farms in my country

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  • Chicken subunit four-combination vaccine and preparation and application thereof
  • Chicken subunit four-combination vaccine and preparation and application thereof
  • Chicken subunit four-combination vaccine and preparation and application thereof

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Embodiment Construction

[0014] The main structural protein VP2 protein of IBDV is the most important host protective antigen. The present invention amplifies the super-virulent VP2 gene of IBD by PCR method, clones it on the pET-28a vector, transforms BL21(DE3), and induces it with IPTG After the VP2 protein is expressed, the expressing cells are homogenized under high pressure and then centrifuged to obtain the supernatant, which is the VP2 protein solution.

[0015] Newcastle disease virus Clone30 strain was inoculated into 10-day-old SPF embryos, and the chicken embryo liquid cultured for 48-96 hours was collected. The virus liquid was concentrated by ultrafiltration and concentration, and 0.1% formaldehyde was added to the final concentration.

[0016] Chicken infectious bronchitis virus M42 strain was used to inoculate 10-11-day-old SPF embryos, and the chicken embryo fluid cultured for 24-48 hours was collected. The virus was concentrated by ultrafiltration and concentration, and 0.1% formaldehy...

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Abstract

The invention aims to prepare a chicken subunit four-combination vaccine which is capable of simultaneously preventing newcastle disease, infectious bronchitis, avian influenza, infectious bursal disease and particularly super-strong virus infection. The vaccine comprises infectious bursal disease VP2 protein with the sequence of SEQ ID NO:1, newcastle disease virus, infectious bronchitis virus and avian influenza virus. The four-combination vaccine can be applied to vaccine immunity of chickens of 21 days, can be used for simultaneously preventing the newcastle disease, the infectious bronchitis, the avian influenza and the infectious bursal disease, has immune effects similar to those of a single newcastle disease vaccine, the inactivated vaccine of the infectious bronchitis, the inactivated vaccine of the avian influenza and single infectious bursal disease vaccine, and fulfills the aims of reducing immunization frequency and lowering stress.

Description

technical field [0001] The invention belongs to the field of poultry vaccine production, and in particular relates to a chicken subunit quadruple vaccine and its preparation and application, that is, a Newcastle disease-infectious bronchitis-bird flu-infectious bursal disease quadruple vaccine. Background technique [0002] Newcastle disease (ND) is an acute infectious disease of poultry caused by Newcastle disease virus (NDV). Although the Newcastle disease live vaccine is effective, cheap and easy to use, it has a short immunization period and is easily interfered by maternal antibodies, and it is easy to cause respiratory side effects and interfere with laboratory diagnosis of virus isolation. Therefore, since 1980, Newcastle disease inactivated vaccines have been used Vaccines have gradually become one of the conventional methods for preventing and controlling Newcastle disease in chicken farms in my country. [0003] Chicken infectious bursal disease (Infectious Bursal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61K39/17A61K39/145A61K39/12A61P31/14A61P31/16
Inventor 王雷凌红丽于春梅吴晓燕
Owner QINGDAO VLAND BIOTECH INC
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