Application of chitosan in avian vaccine composition preparation

A technology of vaccine composition and chitosan solution, which is applied in the application field of poultry vaccine composition, can solve the problems of increasing vaccine cost, increasing animal stress and drug residue, and achieving improved resistance, less antigenicity, The effect of reducing side effects

Inactive Publication Date: 2013-03-20
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Newcastle disease, avian influenza, infectious bronchitis, infectious bursa and other poultry vaccines use oil adjuvants, which need to add more antigens to achieve the immune effect. This has two defects: one is to increase the cost of the vaccine , Second, more vaccines need to be injected to achieve immune protection, which increases animal stress and drug residues

Method used

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  • Application of chitosan in avian vaccine composition preparation
  • Application of chitosan in avian vaccine composition preparation
  • Application of chitosan in avian vaccine composition preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation that contains the chitosan solution of 2%W / V:

[0021] Dissolve 4.1 g of sodium acetate (purchased from Sigma Chemical Co., St. Louis, MO) in 50 ml of deionized water for 0.5 M sodium acetate solution, adjust the pH to 4.5 with about 7 ml of glacial acetic acid, and then add 1.5 ml of glacial acetic acid to compensate for polymerization. The effect of the addition of glucosamine on the pH of the solution. The total volume of the solution was adjusted to 100ml with deionized water.

[0022] 2g of chitosan (product of Shanghai Qisheng Biological Products Co., Ltd., degree of polymerization 88.5%) was slowly added to the above sodium acetate solution and stirred continuously for 2-3 hours until the chitosan was dissolved. Filter through a 0.22 μm membrane to remove impurities, autoclave at 112°C for 30 minutes, and store at 4°C.

Embodiment 2

[0023] Embodiment 2: the application of chitosan in the adjuvant of preparation Newcastle disease vaccine composition

[0024] 1. Newcastle disease virus (hereinafter referred to as NDV) Lasota strain propagation and identification

[0025] 1.1 Newcastle disease virus Lasota strain of breeding chickens was purchased from China Veterinary Drug Control Institute. Properly dilute the virus species with sterilized physiological saline (10 -3 ~10 -4 ), inoculate 10-day-old SPF embryos at 0.1ml per allantoic cavity, culture them for 120 hours, photograph the embryos once every 4-6 hours, take out the dead embryos in time, and select the ones that died 72-120 hours after inoculation and had obvious lesions. As for the chicken embryo, the chicken embryo liquid is harvested separately and put into a sterilized container. The chick embryo fluid tested for sterility and HA ≥ 1:512 was mixed, quantitatively dispensed, 2ml per bottle, and stored in a freezer.

[0026] 1.2 Determination...

Embodiment 3

[0050] Embodiment 3, the application of chitosan in the adjuvant of preparation bird flu vaccine composition

[0051] 1. Propagation and identification of H9HL strain virus species

[0052] 1.1 Breeding of virus seeds: Avian influenza virus A / Chicken / Henan / Luoyang / HL / 2001 (H9N2) (referred to as HL strain). The HL strain freeze-dried virus was properly diluted with sterilized physiological saline (10 -3 ~10 -4 ), 0.1ml per allantoic cavity was inoculated with 10-day-old SPF embryos, and incubated at 37°C for 96 hours. During this period, the dead embryos before 48 hours were discarded. After 48 hours, the embryos were photographed once every 4 to 6 hours, and the dead embryos were removed in time. After 96 hours, take them all out, put them in the refrigerator at 2-8°C for more than 12 hours, harvest the chicken embryo fluids separately, put them into sterilized containers, mix the chicken embryo fluids that have been tested for sterility and have a HA≥1:512, and pack them qu...

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Abstract

The invention relates to an application of chitosan in avian vaccine composition preparation. An avian vaccine composition comprises newcastle disease inactivation virus or avian influenza inactivation virus and other avian vaccine antigens, and a chitosan solution, wherein the avian vaccine comprises a water phase and an oil phase, the chitosan solution is in the water phase, and a concentration of the chitosan solution is 0.1-0.5% W/V, most preferably 0.2-0.5% W/V. According to the avian vaccine composition, the amount of the antigen is low so as to reduce side reactions of the avian vaccine and reduce avian vaccine cost, such that the avian vaccine composition such as newcastle disease vaccines or avian influenza vaccines and the like are suitable for industrial production.

Description

technical field [0001] The present invention relates to the application of chitosan in the preparation of poultry vaccine composition, more specifically relates to the application of chitosan in the preparation of poultry vaccine composition such as Newcastle disease vaccine or avian influenza vaccine. Background technique [0002] Vaccine adjuvants are used for two purposes: they slow the release of antigens from the injection site, and they stimulate the immune system. Traditional vaccines usually consist of crude preparations of inactivated or killed or modified live pathogenic microorganisms. Impurities associated with cultures of these pathogenic microorganisms can act as adjuvants to enhance the immune response. Adding an adjuvant, however, allows a smaller dose of antigen to be used to stimulate a similar immune response, reducing the cost of vaccine manufacture. Thus, the efficacy of certain injectable agents can be significantly increased when the agent is combine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61K39/145A61K39/17A61K39/265A61P31/14A61P31/16
Inventor 张许科孙进忠
Owner PU LIKE BIO ENG
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