Preparation method of anti-neweastle disease virus specific transfer factor and oral liquid, and use thereof

Abstract

The invention provides a preparation method of an anti-neweastle disease virus specific transfer factor. The method comprises the following steps: selecting a healthy chicken as an experiment chicken to carry out supplementary immunization of a newcastle disease inactivated vaccine; monitoring the level of an immune antibody of the newcastle disease vaccine inside the experiment chicken body; taking splenic organs of the experiment chicken in a sterile condition when the level of the antibody achieves 28-29; and processing the splenic organs of the chicken, so as to obtain the anti-neweastle disease virus specific transfer factor. By adopting the anti-neweastle disease virus specific transfer factor disclosed by the invention, the specific immune effect of the chicken body on a neweastle disease virus antigen can be improved; normal cells of the body can be prevented from being infected by the virus. Thus, the transfer factor disclosed by the invention can play the roles in preventing the neweastle disease virus infection and protecting normal cells of the body from being damaged by the neweastle disease virus, so as to reduce the morbidity; meanwhile, the chicken infected by the neweastle disease virus can be treated by using the transfer factor. Therefore, the clinical symptoms can be effectively improved; the recovery rate is increased; the mortality is reduced.

Description

technical field [0001] The invention relates to the field of immunology, in particular to a preparation method and application of anti-chicken Newcastle disease virus specific transfer factor and oral liquid. Background technique [0002] Chicken Newcastle disease (ND), also known as Asian chicken plague, is a highly contagious and fatal disease caused by avian parainfluenza virus Newcastle disease virus (NDV), which mainly affects chickens, turkeys, wild birds and ornamental birds. After the onset of the disease, the chickens showed dyspnea, coughing and asthma, watery feces, and severe cases died. The biggest feature of Newcastle disease virus is that it is easy to mutate, causing the anti-Newcastle disease virus specific antibody that has appeared in chickens to lose the effect of neutralizing the virus, thus making chickens susceptible to infection and even causing large-scale epidemics. [0003] Although there are some pharmaceutical preparations against Newcastle dise...

AI Technical Summary

Problems solved by technology

The raw materials are made by pretreatment, mincing, homogenization, repeated freezing and thawing, centrifugation, dialysis, centrifugation, filtration and other processes. The traditional production process will destroy the bioactive components of the raw materials, the utilization rate of active components is low, and the production cycle is long , it takes at least 7 days

In addition, the mincing process in the traditional process mostly uses a vertical colloid mill, the inactivation is mostly at 37°C for 24 hours, the freeze-thaw process is mostly at -20°C for more than 8 times, and the centrifugal separation is mostly at 4°C, / min centrifugation for 15min, low efficiency, product variability

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